In vitro culture of bovine preantral follicles: a review

Araújo, Valdevane Rocha; Gastal, M.O.; Figueiredo, J.R.

doi:10.1186/1477-7827-12-78

Cited by 95 publications

(64 citation statements)

References 132 publications

(261 reference statements)

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“…The lack of standardized protocols may affect in vitro follicle culture and can also explain the different results from several research groups. Among the commercial culture media, TCM-199 and α-MEM have been the most commonly used to maintain follicular survival and viability and to improve the development of bovine follicles (Araújo et al, 2014). In vitro culture media have nevertheless improved significantly in the last 15 years.…”

Section: Resultsmentioning

confidence: 99%

“…Recent defined IVF media result in embryos that survive slow freezing protocols making it possible to apply direct transfer techniques just like in vivo produced embryos (Blondin, 2015). Multiple variations of media have been tested over time (Araújo et al, 2014) and nowadays the range of products and combinations that provide acceptable results is relatively wide.…”

Section: Resultsmentioning

confidence: 99%

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In Vitro Production of Embryos at Research and Development Station for Cattle Breeding Dancu, Iasi – First Bovine Embryos Produced In Vitro in North-Eastern Romania

Borş¹,

Creangă²,

Dascalu³

et al. 2017

Cercetari Agronomice in Moldova

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ABSTRACT. The in vitro production (IVP) of bovine embryos increases the selection intensity in cattle and reduces the generation interval, which is very important in the genetic gain. In Romania, this reproductive biotechnology has shown a timid evolution in the last years, although the need for genetic improvement in the area is present. The aim of this paper is to describe the work that resulted in first bovine embryos obtained through IVP in North-Eastern Romania. Oocytes were collected by slashing ovaries from slaughtered cows, matured in a TCM199-based medium and fertilized in TL-based medium microdrops with sperm processed by swim-up procedure. The presumptive embryos were cultured one day in TCM199 and 8 days in SOF-based medium and evaluated in days 7, 8 and 9 after fertilization. We retrieved an average number of 83 usable oocytes/IVF session, which represents 73.8% from the total harvested oocytes. The average number of cleaved embryos was 50.8 per IVF, reflecting an average cleavage rate of 61.2%. An average of 8.6 blastocysts/IVF session was obtained, representing 10.4% of the selected oocytes or 16.9% of the number of cleaved embryos. Although suboptimal, the results were comparable with other reports on IVP in cattle. The adapted IVP protocol, based on maturation with TCM199, fertilization in microdrops of TL and culture of presumptive embryos one day in TCM199 and afterwards in SOF seems to offer acceptable results and will be used for further attempts to produce bovine embryos.Keywords: IVP; TCM199 medium; TL medium; SOF medium; swim-up. REZUMAT. Producerea in vitro S.I. BORŞ ET AL.110 creștere a intensității de selecție a bovinelor și o reducere a intervalului între generații, elemente importante în realizarea câștigului genetic. În România, această biotehnică de reproducere asistată la taurine a prezentat o evoluție timidă în ultimii ani, deși există o nevoie crescută în ceea ce privește ameliorarea șeptelului de bovine pentru lapte. Scopul acestui studiu este de a descrie rezultatele obținute ca urmare a cercetărilor efectuate pentru producerea primilor embrioni in vitro la specia Bos taurus în zona de Nord-Est a României. Ovocitele au fost recoltate prin realizarea unor incizii multiple în corticala ovarelor provenite de la vaci sacrificate în abator, apoi maturate în mediul TCM 199 și fertilizate în mediul TL, cu material seminal procesat prin metoda swim-up. Embrionii au fost apoi cultivați o zi în mediul TCM 199 și 8 zile în mediul SOF, evaluarea lor făcându-se pe parcurs în zilele 7, 8 și 9 după fertilizare. S-a obținut o medie de 83 ovocite de bună calitate recoltate/sesiune fertilizare in vitro (FIV), ceea ce reprezintă 73,8% din totalul ovocitelor recoltate. Numărul mediu de embrioni divizați a fost de 50,8/sesiune FIV, ceea ce reflectă o rată de clivaj de aproximativ 61,2%. Din totalul acestora s-a obținut un număr mediu de 8,6 blastociști/sesiune FIV, reprezentând 10,4% din totalul ovocitelor selectate sau 16,9% din numărul de embrioni clivați. Deși rezultatele nu sunt optime, pot f...

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

In Vitro Production of Embryos at Research and Development Station for Cattle Breeding Dancu, Iasi – First Bovine Embryos Produced In Vitro in North-Eastern Romania

Borş¹,

Creangă²,

Dascalu³

et al. 2017

Cercetari Agronomice in Moldova

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“…They produce numerous cytokines and steroid hormones to support oogenesis and self-organization via paracrine and autocrine signaling 72. These factors have not been comprehensively identified; accordingly, the culture of ovaries and/or follicles has been adopted for establishing an in vitro system, rather than the culture of oocytes alone, without their surrounded follicle cells, in livestock, rodents, nonhuman primates and humans 4,24,39,92,96…”

Section: Completion Of Mouse Oogenesis In Vitromentioning

confidence: 99%

“…Compared to the culture of immature oocytes embedded in the primordial or secondary follicles, a greater number of events in oogenesis need to be achieved in PGC culture 4,24,92,96. For example, prior to switching from mitosis to meiosis, female germ cells form cysts via incomplete cytokinesis.…”

Section: Completion Of Mouse Oogenesis In Vitromentioning

confidence: 99%

Differentiation of Mouse Primordial Germ Cells into Functional Oocytes In Vitro

2017

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Various complex molecular events in oogenesis cannot be observed in vivo. As a bioengineering technique for female reproductive tissues, in vitro culture systems for female germ cells have been used to analyze oogenesis and preserve germ cells for over 20 years. Recently, we have established a new methodological approach for the culture of primordial germ cells (PGCs) and successfully obtained offspring. Our PGC culture system will be useful to clarify unresolved mechanisms of fertility and sterility from the beginning of mammalian oogenesis, before meiosis. This review summarizes the history of culture methods for mammalian germ cells, our current in vitro system, and future prospects for the culture of germ cells.

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“…The in vitro follicle culture studies have been performed either using primordial follicles enclosed in ovarian slices (in situ culture) or in the isolated form [1]. Such follicle culture systems have been used respectively to investigate in vitro early and late folliculogenesis at preantral follicle stage [2].…”

mentioning

confidence: 99%

Justicia insularis Improves the in vitro Survival and Development of Ovine Preantral Follicles Enclosed in Ovarian Tissue

Mbemya¹,

Guerreiro²,

Donfack³

et al. 2017

JPP

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Objectives: Evaluating the addition effect of J. insularis extract and FSH on the survival, activation and ROS production after in vitro culture of ovine preantral follicles enclosed in ovarian tissue. Methods: In the first experiment, ovarian fragments were fixed (non-cultured control) or in vitro cultured in α-MEM + (cultured control), α-MEM + supplemented with FSH 50 ng/mL, or in α-MEM + supplemented with J. insularis (JUS0.3; 1.25 or 5 mg/mL) for 1 or 7 days, at 39˚C, 5% CO 2 . In the second experiment, fragments were fixed or cultured in α-MEM + supplemented with anethole 300 µg/mL + FSH 50 ng/mL or in α-MEM + supplemented with anethole 300 µg/mL + 0.3 mg/mL JUS. Key findings: JUS0.3 was the only treatment that maintained the percentage of morphologically normal follicles similar to non-cultured control even after 7 days of culture. After 7 days of culture, a higher (p < 0.05) percentage of developing follicles was observed in JUS5 treatment compared with the other treatments except JUS1.25. In the second experiment, FSH maintained the percentage of normal follicles and promoted activation of primordial follicles. A reduction (p < 0.05) of stromal cell density was observed in MEM + +ANE supplemented with JUS or FSH. Conclusions: J. insularis in a concentration-dependent manner maintained the levels of ROS and improved in vitro follicular survival and activation of ovine primordial follicles.

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In vitro culture of bovine preantral follicles: a review

Cited by 95 publications

References 132 publications

In Vitro Production of Embryos at Research and Development Station for Cattle Breeding Dancu, Iasi – First Bovine Embryos Produced In Vitro in North-Eastern Romania

In Vitro Production of Embryos at Research and Development Station for Cattle Breeding Dancu, Iasi – First Bovine Embryos Produced In Vitro in North-Eastern Romania

Differentiation of Mouse Primordial Germ Cells into Functional Oocytes In Vitro

Justicia insularis Improves the in vitro Survival and Development of Ovine Preantral Follicles Enclosed in Ovarian Tissue

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