GC/MS Profiling, Anti-Collagenase, Anti-Elastase, Anti-Tyrosinase and Anti-Hyaluronidase Activities of a Stenocarpus sinuatus Leaves Extract

Abstract

Today, skin care products and cosmetic preparations containing natural ingredients are widely preferred by consumers. Therefore, many cosmetic brands are encouraged to offer more natural products to the market, such as plant extracts that can be used for their antiaging, antiwrinkle, and depigmentation properties and other cosmetic purposes. In the current study, the volatile constituents of the hexane-soluble fraction of a Stenocarpus sinuatus (family Proteaceae) leaf methanol extract (SSHF) were analyzed using GC/MS analysis. Moreover, the antiaging activity of SSHF was evaluated through in vitro studies of anti-collagenase, anti-elastase, anti-tyrosinase, and anti-hyaluronidase activities. In addition, an in silico docking study was carried out to identify the interaction mechanisms of the major compounds in SSHF with the active sites of the target enzymes. Furthermore, an in silico toxicity study of the identified compounds in SSHF was performed. It was revealed that vitamin E (α-tocopherol) was the major constituent of SSHF, representing 52.59% of the extract, followed by γ-sitosterol (8.65%), neophytadiene (8.19%), β-tocopherol (6.07%), and others. The in vitro studies showed a significant inhibition by SSHF of collagenase, elastase, tyrosinase, and hyaluronidase, with IC50 values of 60.03, 177.5, 67.5, and 38.8 µg/mL, respectively, comparable to those of the positive controls epigallocatechin gallate (ECGC, for collagenase, elastase, hyaluronidase) and kojic acid (for tyrosinase). Additionally, the molecular docking study revealed good acceptable binding scores of the four major compounds, comparable to those of ECGC and kojic acid. Besides, the SSHF identified phytoconstituents showed no predicted potential toxicity nor skin toxicity, as determined in silico. In conclusion, the antiaging potential of SSHF may be attributed to its high content of vitamin E in addition to the synergetic effect of other volatile constituents. Thus, SSHF could be incorporated in pharmaceutical skin care products and cosmetics after further studies.

In Silico Docking, Resistance Modulation and Biofilm Gene Expression in Multidrug-Resistant Acinetobacter baumannii via Cinnamic and Gallic Acids

“…The docking study was conducted to demonstrate the binding affinities of the tested compounds to the active sites of the protein [ 80 , 81 ]. The study was performed using Molecular Operating Environment (MOE 2019.02) software [ 82 , 83 ].…”

Cyclodepsipeptides: Isolation from Endophytic Fungi of Sarcophyton ehrenbergi and Verification of Their Larvicidal Activity via In-Vitro and In-Silico Studies

“…This procedure includes the arrangement of the 3D coordinate space on the binding site of the target, calculation of the binding affinity of the formed complex, and elaboration of the subsequent orientation of a molecule on the ligand’s binding site. The largest magnitude negative number signifies the sensitivity of binding affinity, representing the most satisfactory conformation of the formed complex [ 24 , 25 , 26 , 27 ]. Here, we reveal the isolation and larvicidal activity of two cyclodepsipeptides, scopularide A and B, along with the crude extracts, from the culture rice media of both endophytic A. flavus and A. nomius , isolated from Sarcophyton ehrenbergi .…”

Effect of Atmospheric-Pressure Plasma on Functional Compounds and Physiological Activities in Peanut Shells

“…As with the antioxidant activity mentioned above, these skin aging-related enzyme inhibitory effects of AP plasma treatments are probably due to the polyphenols in peanut shell extract. In previous studies, many anti-aging products were reported in green tea, grape seed, coconut, and argan extracts [ 21 , 25 ]; however, the skin aging-related enzyme inhibitory activities of peanut shell have not yet been elucidated. Recently, many cosmetic companies have shown an interest in natural substances that have anti-tyrosinase, anti-elastase, and anti-collagenase activities.…”