GC-MS profiling and DPPH radical scavenging activity of the bark of Tampoi (Baccaurea macrocarpa)

Abstract

Background : Tampoi ( Baccaurea macrocarpa) is a tropical rainforest plant that produces edible fruit and is native to Southeast Asia, especially East Kalimantan, Indonesia. Previous research showed that Tampoi potentially can be developed as a drug. It was reported that the extract of Tampoi fruit displayed antioxidant activity, which was correlated with its phenolic and flavonoid substances. There is no information about the antioxidant activity of other parts of this plant, such as the bark, which might also have this kind of activity. Therefore, the aim of this study was to evaluate the phytochemical using GC-MS analysis, toxicity againt Artemia salina, and antioxidant activity with DPPH radical scavenging method of the bark of Tampoi. Methods: The bark of Tampoi was extracted with methanol and concentrated using rotary evaporator to obtain the methanol extract of the bark. Secondary metabolites of this extract was determined using phytochemical analysis. Afterward, the methanol extract was tested for its toxicity using brine shrimp lethality test and antioxidant activity using the 2,2-diphenyl-1-picrylhydrazyl method. Results : Phytochemical evaluation results showed that the methanol extract of bark of this plant contains several secondary metabolites including alkaloids, flavonoids, phenolics, steroids, and triterpenoids. The toxicity test displayed no toxic property due to a LC 50 value above 1000 ppm. For antioxidant activity, the result exhibited that the methanol extract of bark of this plant could be categorized as an active extract with IC 50 value of 11.15 ppm. Moreover, based on gas chromatography-mass spectrometer analysis, there are 37 isolated compounds from the bark, one of which is methylparaben, a phenolic predicted to act as an antioxidant. Conclusion: The results obtained in this research demonstrated that the bark of Tampoi ( B. macrocarpa) has potential as an antioxidant.

Formulation and Physical Properties Test of Nano Cream Preparation Purified Extract of Kenikir Leaf (Etdk) and Tampoi Fruit Peel Extract (Ekbt)

“…The Baccaurea species be inspected contain secondary metabolites, including saponins, flavonoids, alkaloids, phenols, anthocyanins, and carotenoids. 4,5 Research on 6 The Tampoi bark has antioxidant activity, in line with the research 7,8 which states that Tampoi peel extract has antioxidant activity. The methanol extract of the Tampoi fruit peel contains alkaloid compounds, polyphenols, and flavonoids.…”

Baccaurea Lour. (Phyllanthaceae Martinov - Malpighiales), underutilized plant from Riau, Indonesia and its phytochemical study

“…In this study, steroid is absent in all tested specimens. However, the presence of steroid in bark of B. macrocarpa had been reported by Erwin et al (2019) and B. courtallensis (Sivadasan et al 2020).…”