Gastroprotective effect of ghrelin against indomethacin-induced gastric injury in rats: possible role of heme oxygenase-1 pathway

Allam, Mona M.; El-Gohary, Ola A

doi:10.4149/gpb_2016056

Cited by 15 publications

(21 citation statements)

References 45 publications

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“…caused significant ulceration in the glandular area and degenerative changes in gastric tissues of the rat stomach as seen in histopathological examination as it showed epithelial loss, congested blood vessels with inflammatory cell infiltration, associated with considerable elevation in ulcer number and ulcer index, which was in agreement with previous studies that indicated that NSAIDs like indomethacin can generate observable gastric ulcers in experimental animals [1,6,18,39,75,89,90]. Pyloric-ligation/indomethacin-induced mucosal injury is attributed to various processes, including infiltration of leukocytes, inhibition of PG-E 2 , initiation of lipid peroxidation, decreasing levels of anti-oxidants, and induction of apoptosis [91,92].…”

Section: Discussionsupporting

confidence: 90%

Gastro-Protective and Anti-Oxidant Potential of Althaea officinalis and Solanum nigrum on Pyloric Ligation/Indomethacin-Induced Ulceration in Rats

et al. 2019

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Recently, an alternative disease treatment approach is the research of medicaments from traditional medicine. Plants with anti-oxidant capabilities are used as herbal treatments for ulcer diseases. Medicinal/herbal extracts containing phytoconstituents have significant anti-ulcer activities in in vivo experiments on animal models, compared to reference drugs. The current study aims to inspect gastro-protective as well as in vitro and in vivo anti-oxidant potential of Althaea officinalis and Solanum nigrum extracts on pyloric-ligation/indomethacin-induced gastric-ulceration in rats. Rats were divided into six groups: normal control, gastric ulcer control, two standard pretreatment groups receiving omeprazole and misoprostol, and two test pretreatment groups receiving Althaea officinalis and Solanum nigrum. Pretreatments were administrated orally for 14 days. On the 15th day, animals, excluding the normal control group, were exposed to pyloric-ligation followed by indomethacin injection. After four hours, the rat’s stomachs were removed and gastric juice and blood samples were collected. Pyloric-ligation/indomethacin administration caused considerable elevation in ulcer number, ulcer index, acid and pepsin productivity, aggressive factors, and gastric mucosal lipid-peroxide contents. Moreover, reduction in titratable acidity, gastric mucosal nitric-oxide, anti-oxidant contents, and protective factors accompanied gastric-ulceration. Additionally, elevation in pro-inflammatory cytokines content and reduction in cystathionine-β-synthase and heme-oxygenase-1 expression was witnessed. Omeprazole, misoprostol, Althaea officinalis, and Solanum nigrum pretreatments fixed blood and tissue biomarkers, thereby protecting them from pyloric-ligation/indomethacin-induced gastric-ulceration in rats, which is hopeful for clinical examinations.

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Section: Discussionsupporting

confidence: 90%

Gastro-Protective and Anti-Oxidant Potential of Althaea officinalis and Solanum nigrum on Pyloric Ligation/Indomethacin-Induced Ulceration in Rats

et al. 2019

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“…Injection of indomethacin significantly raised the concentration of MDA in the mucosa of the stomach about three times, reaching (16.76 ±2.21 nmol/g tissue) comparing with the control group (5.29±1.44 nmol/g tissue) .These results agree with many authors (Kim et al,2011 ;Allam andEL-Gohary, 2017 andNwaonukuru et al,2018) .BL pretreatment (100 and 200 mg/kg b.w respectively) significantly decreased MDA to 10.36 ± 1.97nmol/g and 9.10±0.81nmol/g when compared to indomethacin group. BF pretreatment (100 and 200 mg/kg b.w respectively) diminished MDA to 7.96±0.77 nmol/g and 6.15±0.55 wet tissue, respectively.While RAN pretreatment diminished MDA to 6.83±0.76 nmol/g tissue, Mixture extracts BL and BF altogether expanded the reduction of MDA to 6.62 ±1.47nmol/g tissue.Ranitidine has often been accounted to possess immunosuppressive actions and antioxidant , which might be in charge of its antiulcerogenic activity (Ardestani et al, 2004) .Borago introduced a noticeable repression of oxidative damage because of its phenomenal radical scavenging capacity, It conveyed MDA level nearer to ordinary levels.…”

Section: Oxidative Stresssupporting

confidence: 91%

Gastroprotective Effect of Borago Extracts against Indomethacin-Induced Gastric Ulcer in Rats

Gazwi

Mahmoud

2018

Journal of Food and Dairy Sciences

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Gastric ulcer stays one of the perilous sicknesses in the world. In spite of the fact that there are numerous medications utilized for the treatment of gastric ulcer, the vast majority of these deliver a few adverse impacts. The aim of this investigation was to assess the antiulcer effects of Borago (Borago officinalis L) leaves extract (BL), flowers extract (BF) and the mixture of leaves and flowers extracts (Mix) against gastric ulcer induced by indomethacin in rats and compared with ranitidine as a reference drug. Gastric ulceration was stimulated by indomethacin at one dose (30 mg/kg b.w. Intraperitoneal (i.p)). BL and BF were administered orally at two doses of 100 and 200 mg/kg b.w, Mix was given orally at a dose of 200 mg/kg b.w. and ranitidine at a dose of 20 mg/kg b.w. for three weeks before the injection of indomethacin. The results appeared that indomethacin significantly decreased pH value, mucin content, catalase activity (CAT) and nitric oxide (NO), while, increased ulcer index, total acidity, free acidity and malondialdehyde (MDA) comparing with control group.While, pretreatment with Borago extracts provided a significant decrease in ulcer index, total acidity,free acidity, MDA, and associated with a significant rise in pH value, mucin content, CAT and NO compared to indomethacin group. The protection provided by the extracts of Borago is similar to ranitidine. Histological studies of stomach mucosa affirmed these outcomes. Borago has protective against indomethacin which caused gastric ulcer because of it contains antioxidant properties. The results of the present suggest that Borago can be utilized against gastric ulcer impacts as well as to decrease antagonist drugs adverse effects.

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“…Oxidant and antioxidant parameters are used to evaluate the biochemical effect of Indo on the stomach (Allam and El-Gohary 2017). As can be conducted from present results, oxidant parameter levels such as MDA in the Indo group with larger UI showed a significant increase compared to the healthy, or for groups treated with fruit juices highly significant decrease of NO and GSH in gastric tissues.…”

Section: Discussionsupporting

confidence: 51%

Anti-ulcer Activity of Avocado Juice and Kiwi Juice on Indomethacin Induced Gastric Ulcer in Rats

allah

Amira²

2019

Egyptian Journal of Nutrition and Health

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Over past decades, physicians have recommended dietary adjustments, aimed at preventing or treating symptoms of peptic ulcer, as a diet that moderates the risk of stomach ulceration. This study aims to investigate the effect and mechanism of consumption of avocado (Persea americana Mill) juice and kiwi (Actinidia deliciosa) juice to alleviate peptic ulcer in rats. Thirty-six male albino rats (185±10 g), were used and divided into 6 equal groups, one was kept as a (-ve) control group, while the other groups were received daily oral dose of indomethacin (25mg / kg b.Wt.) once daily for 21 days. One group received Indo only (+ve control group), while the other groups given orally (1.5, 3 ml per day) of each juice for 21 days plus Indo. Peptic ulcer index, preventive index, and acidity of gastric juice were assessed. Nitric oxide (NO), malondialdehyde (MDA) and glutathione (GSH) were measured in gastric tissue, as well as histopathological changes in the gastric mucosa. Treatment with avocado juice and kiwi juice resulted in amelioration of the gastric histological lesions induced by Indo. Moreover, fresh juices of avocado and kiwi significantly modulated biochemical marker of oxidative/nitrosative stress markers; malondialdehyde (MDA), and nitric oxide (NO) and antioxidant enzymes as glutathione (GSH), which were significantly disrupted in animal treated with Indo alone. According to this study, the fresh juices of avocado and kiwi could protect against peptic ulcer and its other side effects. This may be due to the high content of phenolic and flavonoid compounds in these juices.

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Gastroprotective effect of ghrelin against indomethacin-induced gastric injury in rats: possible role of heme oxygenase-1 pathway

Cited by 15 publications

References 45 publications

Gastro-Protective and Anti-Oxidant Potential of Althaea officinalis and Solanum nigrum on Pyloric Ligation/Indomethacin-Induced Ulceration in Rats

Gastro-Protective and Anti-Oxidant Potential of Althaea officinalis and Solanum nigrum on Pyloric Ligation/Indomethacin-Induced Ulceration in Rats

Gastroprotective Effect of Borago Extracts against Indomethacin-Induced Gastric Ulcer in Rats

Anti-ulcer Activity of Avocado Juice and Kiwi Juice on Indomethacin Induced Gastric Ulcer in Rats

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