Gastric Parietal Cell and Other Autoantibodies in the BB Rat

Elder, Melissa E.; Maclaren, Noel K.; Riley, William M.; McConnell, Thomas J.

doi:10.2337/diab.31.4.313

Cited by 49 publications

(32 citation statements)

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“…In the spontaneously diabetic BB rat, we have also demonstrated similarly that gastric parietal cell autoantibodies were detectable before or near the age of onset of diabetes with little increase in the frequency of gastric parietal cell autoantibodies thereafter [15]. These results raise the possibility that both Type I diabetes and other associated autoimmune diseases result from a common process.…”

Section: Discussionsupporting

confidence: 52%

Coincident presence of thyro-gastric autoimmunity at onset of Type 1 (insulin-dependent) diabetes

1983

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Summary.We have determined thyroid microsomal and gastric parietal cell autoantibodies in 972 patients with onset of Type 1 (insulin-dependent) diabetes before age 20 years. In a cross sectional study, the frequencies of these autoantibodies did not change significantly over the ensuing 20 years following diagnosis of diabetes. In a longitudinal study of 424 patients with Type 1 diabetes followed up to 5.3 years, few new patients with thyro-gastric autoantibodies were identified. Whereas the frequencies of these autoantibodies among the diabetic patients were more than fivefold greater than for age, sex and race matched controls, they resembled those seen in the non-diabetic general population (n=1524) for the over 60 year age group. We conclude that in patients with thyrogastric autoantibodies and Type 1 diabetes, the onset of autoimmune processes affecting the thyroid, gastric mucosa and pancreatic islets tend to be simultaneous, although the resultant diseases have different natural histories.

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Section: Discussionsupporting

confidence: 52%

Coincident presence of thyro-gastric autoimmunity at onset of Type 1 (insulin-dependent) diabetes

1983

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“…Elder et al [15] reported that the BB rat does not have pancreatic cell auto-antibodies. Tingle and Nakhooda (cited by Dyrberg et al) [161 have been unable to demonstrate islet cell cytoplasmic antibodies by immunofluorescence on frozen sections of rat pancreas.…”

Section: Discussionmentioning

confidence: 99%

An immunofluorescence study of anti-pancreatic islet cell antibodies in the spontaneously diabetic BB Wistar rat

Pollard¹,

Gupta²,

Mancino³

et al. 1983

Diabetologia

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Summary. Sera from BB Wistar rats and Wistar control rats were evaluated for the presence of islet cell antibodies in a prospective study using an indirect immunofluorescence assay on pancreatic islet cell suspensions from cultured rat islets. Islet cell surface antibodies were detected in sera from all animals of the spontaneously diabetic BB Wistar rat colony. The antibody could be detected well before the clinical onset of the disease and was present throughout the course of study of all diabetic animals. Sera from control Wistar rats were negative when tested for this antibody.Key words: Islet cell surface antibody, BB rat, immunofluorescence, islet culture, cell suspension, pre-diabetes.A number of investigators have reported antibodies in the sera of patients with insulin-dependent diabetes [1][2][3][4][5]. These earlier studies tested a human serum reaction on frozen or fixed sections from blood group 0 cadaveric donor pancreases. This islet cell antibody is reported to bind cytoplasmic antigens only [6,7], and is found in 60%-85% of newly diagnosed patients with insulin-dependent diabetes. The percentage of patients with detectable levels of circulating islet cell antibodies decreases with time [8].More recently, an islet cell surface antibody has been detected in the sera of diabetic patients [6,9]. Islet cell surface antibody is detected in a majority of insulindependent diabetic patients ( > 60%) and is reported to lyse fl cells specifically from newborn rat islets. However, islet cell surface antibody is not diagnostic of insulin-dependent diabetes as it is found also in a significant proportion of first degree relatives (25 %).The spontaneously diabetic BB Wistar rat presents a syndrome with many analogies to the development and expression of insulin-dependent diabetes in man [10,11]. We have been interested in it as a model for examining the role that antibodies to pancreatic islet cells might play in the aetiology of insulin-dependent diabetes, with particular reference to the questions: (1) does the diabetic rat have a circulating antibody specific for a pancreatic islet cell but not present in the normal rat? and (2) if so, does the presence of this antibody precede the onset of the disease? We report here the results of an immunofluorescence study aimed at answering these questions. Materials and Methods Animals and SeraThe BB rat is maintained in our laboratories as a random-bred, closed colony. Animals are kept in an air-conditioned room (20 ~ 50% relative humidity) with a 12-h light/dark cycle, and fed Purina Lab Chow 5001 and water ad libitum. Urinary glucose levels are determined daily on all BB rats using the glucose enzymatic test strip (Tes-Tape, Eli Lilly, Toronto, Canada). Individual rats are classified as diabetic on the basis of a positive glycosuria and they are subsequently maintained on daily PZ1 insulin. At the time of this study the incidence of diabetes in the colony was 70% and the average age of onset 85 days.Eleven diabetic BB rats from seven litters, all diagnosed as diabe...

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“…Like the increased prevalence of thyroid auto-immunity in patients with IDDM (Gray et al, 1980), in some instances, lymphocytic thyroiditis (LT) was also found in BB/W rat (Sternthal et al, 1981). Moreover many organ specific antibodies, such as islet cell surface antibody (ICSA), gastric parietal cell antibody (PCA), smooth muscle antibody (SMA) and thyroidal colloid antibody (TCA) have been also found in BB/W rat (Dyrberg et al, 1982;Elder et al, 1982;. Therefore it was suggested that BB/W rat represents a new model of multiple autoimmune endocrinopathy (Sternthal et al, 1981).…”

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confidence: 99%