Gastric blood flow in anaesthetized cats

Abstract

SU-MMARY 1. The gastric mucosal blood flow has been measured by the amidopyrine clearance technique in anaesthetized cats. The total gastric blood flow has been (a) measured directly and (b) calculated by the Fick principle from the amidopyrine concentrations in gastric arterial and venous blood and the gastric output of amidopyrine.2. Observations on the recovery of added amidopyrine from arterial and venous blood and plasma, and on the rate of transfer of amidopyrine from corpuscles to plasma, support the underlying assumptions of the amidopyrine method of measuring mucosal blood flow.3. If acid solutions are instilled into the stomach the mucosal blood flow of the non-secreting stomach may be measured by the amidopyrine technique.4. Total gastric and mucosal blood flow increased linearly with increase in H+ secretion, stimulated by histamine or gastrin. The increase in total flow was entirely due to the increase in mucosal flow.5. The relationship between mucosal blood flow and H+ secretion was the same for histamine and gastrin responses, and was unaffected by maintaining the total flow at a constant level, or by reduction of the circulating blood volume. Increase in blood volume altered the relationship so that there was a greater increment in mucosal blood flow for any increase in H+ secretion.6. It is concluded that valid observations may be made on secretion and blood flow relationships in acute anaesthetized preparations.

The effect of splanchnic nerve stimulation on gastric acid secretion and mucosal blood flow in the anaesthetized cat

“…Swan have shown a marked rise in mucosal blood flow associaated with acid secretion stimulated by insulin and 2-deoxy-D-glucose. A parallel rise in mucosal blood flow and gastric acid secretion has been shown when acid secretion is stimulated with histamine Moody, 1967;Harper et al 1968) or gastrin Harper et al 1968;Cowley et al 1969).…”

“…a direct antisecretory effect, this would reduce mucosal blood flow, assuming mucosal blood flow to be controlled by acid secretion. However, it has been shown (Harper et al 1968) that under certain conditions an increase in mucosal blood flow potentiates acid secretion. Therefore, if a rise in central B.P.…”

“…plasma stored for amidopyrine extraction and the R.B.C.s returned to the animal via the indwelling cannula. Gastric mucosal blood flow was measured by the amidopyrine technique described for use in the anaesthetized cat by Harper et al (1968).…”

The effcts of sodium fluoride on gastric acid and electrolyte output in the anaesthetized cat.

“…In a limited number of experiments, the gastric mucosal blood flow was determined by the amidopyrine technique described by Harper, Reed & Smy (1968).…”

The metabolism of [14C]histamine during pentagastrin‐stimulated acid secretion in the cat.

“…The abdomen was opened by a mid-line incision and the pylorus occluded with a tape ligature. The hepatic artery was cannulated retrogradely on the right side of the stomach as described by Harper, Reed & Smy (1968), and the splenic vessels were tied close to the spleen. By this route [14C]histamine solution in saline was given directly into the arterial supply to the stomach, via the coeliac axis.…”