Gas-liquid chromatography as an analytical tool in microbiology

Moss, C W

doi:10.1016/s0021-9673(00)80305-2

Cited by 164 publications

(105 citation statements)

References 31 publications

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“…A fatty acid profile of bacterium is a very stable phenotypic expression of a bacterial genotype when the analysis conditions, from bacterial growth to the fatty acid extraction procedure, are rigorously controiied (Moss, 1981;Stayer and Davis, 1992;Wauihoz, et ai., 1995). Moreover, fatty acid metabolism is directed by chromosomal DNA and the presence of certain fatty acids in bacteria has been shown to correlate with chemotaxonomic characteristics (O'Domell, 1985;Mukwaga and WeIch, 1989;Moss, 199%).…”

Section: Principle Of Gas Liquid Chromatography (Ge)mentioning

confidence: 99%

Identification of contamination sources of Bacillus cereus in pasteurized milk

Lin

Schraft

Odumeru

et al. 1998

International Journal of Food Microbiology

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Section: Principle Of Gas Liquid Chromatography (Ge)mentioning

confidence: 99%

Identification of contamination sources of Bacillus cereus in pasteurized milk

Lin

Schraft

Odumeru

et al. 1998

International Journal of Food Microbiology

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“…The whole cell fatty acid (FA) profiles, mainly in bacteria, have been used as a species identification tool (Moss 1981, Veys et al 1989. The value of FA analysis in yeast taxonomy is of increasing interest; FA composition has been screened and analyzed under several conditions (Menyawi et al 2000).…”

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confidence: 99%

Characterization of Aspergillus species based on fatty acid profiles

Fraga¹,

Santana

Gatti

et al. 2008

Mem. Inst. Oswaldo Cruz

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“…Using some of the same strains, Stanley et al (21) characterized the DNA nucleotide sequence divergences in and proximal to known symbiotic genes and concluded that strains of B . japonicum comprise two symbiotic genotypes (sTI and sTII) consistent with separate species designations.Whole-cell fatty acid profiles of strains grown under standardized conditions have been used for identification (1, 4,18) and classification of many bacteria (10, 17), but not in the genus Bradyrhizobium. Pankhurst et al (20) correlated rifampin resistance in some slow-growing rhizobial strains with in vitro nitrogenase activity.…”

mentioning

confidence: 99%

“…Whole-cell fatty acid profiles of strains grown under standardized conditions have been used for identification (1, 4,18) and classification of many bacteria (10, 17), but not in the genus Bradyrhizobium. Pankhurst et al (20) correlated rifampin resistance in some slow-growing rhizobial strains with in vitro nitrogenase activity.…”

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confidence: 99%

Fatty Acids, Antibiotic Resistance, and Deoxyribonucleic Acid Homology Groups of Bradyrhizobium japonicum

Kuykendall

Roy

O'Neill

et al. 1988

International Journal of Systematic Bacteriology

914

621

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The fatty acid compositions and multiple antibiotic resistance patterns of 32 strains of Bradyrhizobium japonicum correlated with two major deoxyribonucleic acid homology groups. In group I, the fatty acid composition was 1.3% 16:l cis9 acid, 3.6% 16:lC acid, 8.8% 16:O acid, 1.2% 19:0 cyclopropane acid, and 81.2% 18:l acid. Group I1 contained 0.5% 16:lC acid, 11.1% 16:O acid, 0.8% 17:O cyclopropane acid, 24.7% 19:O cyclopropane acid, and 62.3% 18:l acid. Group I strains were susceptible to rifampin (500 pglml), tetracycline (100 pglml), streptomycin (100 pg/ml), chloramphenicol (500 pg/ml), erythromycin (250 pglml), carbenicillin (500 pg/ml), and nalidixic acid (50 pg/ml), whereas group I1 strains were resistant to these antibiotics. Both groups were resistant to trimethoprim (50 pg/ml) and vancomycin (100 pglml).Bradyrhizobium japonicum (Jordan 1982) (formerly Rhizobium japonicum) is the slow-growing symbiont of soybeans. This species is comprised of two highly divergent taxonomic groups which should be phenotypically distinguishable. The deoxyribonucleic acid (DNA)-DNA hybridization studies of Hollis et al. (8) established DNA homology groups I, Ia, and 11. These groups were characterized with respect to acidic extracellular polysaccharide composition and phenotypic ex planta nitrogenase expression. Groups I and Ia were reported to be quite distinct from DNA homology group I1 according to both of these criteria (9). Using some of the same strains, Stanley et al. (21) characterized the DNA nucleotide sequence divergences in and proximal to known symbiotic genes and concluded that strains of B . japonicum comprise two symbiotic genotypes (sTI and sTII) consistent with separate species designations.Whole-cell fatty acid profiles of strains grown under standardized conditions have been used for identification (1, 4,18) and classification of many bacteria (10, 17), but not in the genus Bradyrhizobium. Pankhurst et al. (20) correlated rifampin resistance in some slow-growing rhizobial strains with in vitro nitrogenase activity. This observation, together with that of Huber et al. (9) that only DNA homology group I1 strains express nitrogenase in vitro, suggested to us that patterns of antibiotic resistance might also distinguish the groups. In this paper we report that whole-cell fatty acid profiles correlate with DNA homology groups I and I1 of Hollis et al. (8) and that patterns of high-level resistance to antibiotics distinguish the two DNA homology groups. MATERIALS AND METHODSBacterial strains. All USDA strains were obtained from H. H. Keyser and D.Weber, U. S. Department of Agriculture (USDA), Beltsville, Md.; strain ATCC 10324 was obtained from the American Type Culture Collection, Rockville, Md.; strains 5631, 5633, D193, D209, THA6, and 8' were obtained from G. H. Elkan, North Carolina State University, Raleigh; and strains 61A.50 and 61A76 were obtained from S. Smith, Nitragin Co., Milwaukee, Wis. Strains that exhibit rifampin resistance and tetracycline resistance were purified by streaking onto a mediu...

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Gas-liquid chromatography as an analytical tool in microbiology

Cited by 164 publications

References 31 publications

Identification of contamination sources of Bacillus cereus in pasteurized milk

Identification of contamination sources of Bacillus cereus in pasteurized milk

Characterization of Aspergillus species based on fatty acid profiles

Fatty Acids, Antibiotic Resistance, and Deoxyribonucleic Acid Homology Groups of Bradyrhizobium japonicum

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