Characterization of Aspergillus species based on fatty acid profiles

Fraga, Marcelo Elias; Santana, Djalva M.N.; Gatti, Mario Jorge; Direito, Glória Maria; Cavaglieri, Lilia Reneé; Rosa, Carlos Alberto R

doi:10.1590/s0074-02762008000600005

Cited by 23 publications

(19 citation statements)

References 11 publications

(23 reference statements)

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“…The proportion of the detected fatty acids is shown by the FAMEs analysis, indicating that oleic and linoleic acid are more abundant in the cells compared with palmitic and stearic acid in both the TDR and the parent strain. This corresponds with the fatty acid composition observed in other species of Aspergillus (Fraga et al 2008). …”

Section: Discussionsupporting

confidence: 88%

Alkane biosynthesis by Aspergillus carbonarius ITEM 5010 through heterologous expression of Synechococcus elongatus acyl-ACP/CoA reductase and aldehyde deformylating oxygenase genes

et al. 2017

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In this study we describe the heterologous expression of the recently identified cyanobacterial pathway for long chain alkane biosynthesis, involving the reduction of fatty acyl-ACP to fatty aldehyde and the subsequent conversion of this into alkanes, in the filamentous fungus Aspergillus carbonarius ITEM 5010. Genes originating from Synechococcus elongatus strain PCC7942, encoding acyl-ACP/CoA reductase and aldehyde deformylating oxygenase enzymes, were successfully expressed in A. carbonarius, which lead to the production of pentadecane and heptadecane, alkanes that have not been previously produced by this fungus. Titers of 0.2, 0.5 and 2.7 mg/l pentadecane and 0.8, 1.6 and 10.2 mg/l heptadecane were achieved using glucose, Yeast malt and oatmeal media, respectively. Besides producing alkanes, we found elevated levels of internal free fatty acids and triglycerides in the alkane producing transformant. These findings can indicate that a yet unidentified, native fatty aldehyde dehydrogenase channels back the fatty aldehydes into the fatty acid metabolism, thus competing for substrate with the heterologously expressed fatty aldehyde deformylating oxygenase. These findings will potentially facilitate the future application of robust, fungal cell factories for the production of advanced biofuels from various substrates.Electronic supplementary materialThe online version of this article (doi:10.1186/s13568-016-0321-x) contains supplementary material, which is available to authorized users.

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Section: Discussionsupporting

confidence: 88%

Alkane biosynthesis by Aspergillus carbonarius ITEM 5010 through heterologous expression of Synechococcus elongatus acyl-ACP/CoA reductase and aldehyde deformylating oxygenase genes

et al. 2017

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“…Macroscopic observation of the colonies was recorded while microscopic characteristics were studied by scanning electron microscopy (SEM) (Stereoscan440, Leica, Cambridge, UK) and images were obtained at surfaces with 200-4000× magnification. Cellular fatty acid profiling as mentioned below was also used for identification [22,23].…”

Section: Microorganism Used Growth Conditions and Culture Charactermentioning

confidence: 99%

“…Therefore, cellular fatty acids as FAMEs have been used as a taxonomic tool to characterize and differentiate filamentous fungi at the genus and/or species level [22,44]. Individual Aspergillus species have been classified on the basis of fatty acid composition which showed significantly similar fatty acid methyl ester profiles, with 95 % of Aspergillus fatty acids being comprised of palmitic (C16:0), stearic (C18:0), oleic (C18:1), and linoleic acids (C18:2) [23,45,46]. The similar fatty acids were observed in the FAMEs of the isolate IBB G4 grown on glucose (Table 1), which correspond to 91 % of the total fatty acid content typical of reference Aspergillus sp.…”

Section: Fungal Culture Identificationmentioning

confidence: 99%

“…The similar fatty acids were observed in the FAMEs of the isolate IBB G4 grown on glucose (Table 1), which correspond to 91 % of the total fatty acid content typical of reference Aspergillus sp. Thus, based on the morphology [20,21] and FAME profiling [23], the characteristic features suggest strain IBB G4 to be Aspergillus candidus.…”

Section: Fungal Culture Identificationmentioning

confidence: 99%

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Biodiesel Production by Direct In Situ Transesterification of an Oleaginous Tropical Mangrove Fungus Grown on Untreated Agro-Residues and Evaluation of Its Fuel Properties

et al. 2015

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Recent research has focused on use of waste agroresidues for growth of oleaginous microbial biomass as renewable feedstock for biodiesel. However, pretreatment of lignocellulosic biomass into fermentable sugar is necessary for microbial growth, increasing production costs. An oleaginous fungal isolate from the tropical mangrove wetlands, IBB G4, identified as Aspergillus candidus was assessed for its growth on waste agroresidues viz., banana peel, copra meal, corn cob, grape stalks and sugarcane bagasse,which had not been given any thermochemical or enzymatic pre-treatment. The resulting fungal biomass was subjected to in situ (direct) acid transesterification for fatty acid methyl esters (FAME) extraction. Maximal FAME production was obtained on raw untreated banana peel (420 mg/L) and sugarcane bagasse (400 mg/L) with the yields significantly higher (52-68 mg/g of fermented biomass) than those for glucose (32 mg/g). The FAMEs showed major presence of monounsaturated methyl esters (41.7 %; C18:1, C15:1, C17:1, C14:1) and methyl linoleate (C18:2, 36.8 %) and 20 % as saturated fraction when sugarcane bagasse was the substrate for fungal strain. For banana peel, the saturated fatty acid methyl esters (48.6 %; C16:0, C18:0) were abundant, methyl oleate (C18:1, 25 %) was the major monounsaturated fatty ester while methyl linoleate (C18:2, 19 %) and methyl arachidonate (C20:4, 3.8 %) were prevalent as polyunsaturated methyl esters. Biodiesel fuel properties (density, kinematic viscosity, iodine value, cetane number, free and total glycerol) were in accordance with international(ASTM D6751, EN 14214) and national (IS 15607) biodiesel standards, suggesting their suitability as biodiesel fuel.The results in our study indicate the potential suitability of A. candidus IBBG4 biomass grown on raw untreated banana peel and sugarcane bagasse waste and its conversion to FAME by direct in situ acid transesterification. This would help improve process economics for a green and sustainable production of biodiesel.

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“…Chemotaxonomic markers used in the field of mycology include numerous compounds such as amino acids, FAs and carbohydrates [28,29]; in particular, FAs are increasingly being used as a chemotaxonomic tool for the identification and classification of fungi [30]. Numerous researchers have used FAs as chemical markers and auxiliary tools for identifying and differentiating between important fungal species such as Fusarium [30], Aspergillus [31], and Alternaria [32].…”

Section: Introductionmentioning

confidence: 99%

Molecular and biochemical taxonomic tools for the identification and classification of plant-pathogenicPenicilliumspecies

Mahmoud

Abdelaziz

Al-Othman

2016

Biotechnology & Biotechnological Equipment

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Five species of Penicillium (Penicillium chrysogenum, P. funiculosum, P. griseofulvum, P. implicatum and P. oxalicum) are implicated in seed-borne diseases. Here, we report the discovery of molecular markers based on the internal transcribed spacer regions of fungal ribosomal DNA (rDNA), which are described as primary DNA barcode markers of fungi, for rapid diagnosis and early detection of Penicillium spp. The present markers are expected to be useful for the prevention of seedling and systemic plant diseases associated with Penicillium spp. Our findings, which provide valuable insights into the taxonomy of Penicillium spp., should contribute to improve safety of agricultural produce, thereby protecting both humans and animals from harmful food contaminants such as mycotoxins. In addition, we examined the cellular fatty acid composition of five species of Penicillium. The species studied were found to possess similar fatty acid composition; however, they differed in terms of relative concentration. The principal fatty acids were oleic acid (C18:1) and linoleic acid (C18:2), comprising 80% or more of the total fatty acid composition of these species. These fatty acid profiles may be useful for characterization and identification of fungi. Data derived from the present study highlight the importance of using polyphasic methods for accurate specieslevel identification of Penicillium.

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Characterization of Aspergillus species based on fatty acid profiles

Cited by 23 publications

References 11 publications

Alkane biosynthesis by Aspergillus carbonarius ITEM 5010 through heterologous expression of Synechococcus elongatus acyl-ACP/CoA reductase and aldehyde deformylating oxygenase genes

Alkane biosynthesis by Aspergillus carbonarius ITEM 5010 through heterologous expression of Synechococcus elongatus acyl-ACP/CoA reductase and aldehyde deformylating oxygenase genes

Biodiesel Production by Direct In Situ Transesterification of an Oleaginous Tropical Mangrove Fungus Grown on Untreated Agro-Residues and Evaluation of Its Fuel Properties

Molecular and biochemical taxonomic tools for the identification and classification of plant-pathogenicPenicilliumspecies

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