Alkane biosynthesis by Aspergillus carbonarius ITEM 5010 through heterologous expression of Synechococcus elongatus acyl-ACP/CoA reductase and aldehyde deformylating oxygenase genes

Sinha, Malavika; Weyda, Istvan; Sørensen, Annette; Bruno, Kenneth S.; Ahring, Birgitte Kiær

doi:10.1186/s13568-016-0321-x

Cited by 20 publications

(10 citation statements)

References 29 publications

(37 reference statements)

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“…It is known that pomegranate contains sugars such as xylose, arabinose, and glucose [ 62 ]. GC×GC-TOFMS results show that fermentation was conducted successfully due to occurring alcohols (xylitol, sorbitol, and arabinitol) [ 63 ] and fatty acids (linoleic, oleic, and erucic acids) ( Table 3 ) [ 64 ]. Ergosterol, which is a secondary metabolite of Aspergillus spp ., might come from its fungal cell membrane [ 65 , 66 ].…”

Section: Resultsmentioning

confidence: 99%

Production of Bio-Based Pigments from Food Processing Industry By-Products (Apple, Pomegranate, Black Carrot, Red Beet Pulps) Using Aspergillus carbonarius

Arikan

Canlı

Caro³

et al. 2020

JoF

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Food processing industry by-products (apple, pomegranate, black carrot, and red beet pulps) were evaluated as raw materials in pigment production by the filamentous fungi Aspergillus carbonarius. The effect of fermentation conditions (solid and submerged-state), incubation period (3, 6, 9, 12, and 15 d), initial substrate pH (4.5, 5.5, 6.5, 7.5, and 8.5), and pulp particle size (<1.4, 1.4–2.0, 2–4, and >4 mm) on fungal pigment production were tested to optimize the conditions. Pigment extraction analysis carried out under solid-state fermentation conditions showed that the maximum pigment production was determined as 9.21 ± 0.59 absorbance unit at the corresponding wavelength per gram (AU/g) dry fermented mass (dfm) for pomegranate pulp (PP) by A. carbonarius for 5 d. Moreover, the highest pigment production was obtained as 61.84 ± 2.16 AU/g dfm as yellowish brown at initial pH 6.5 with < 1.4 mm of substrate particle size for 15-d incubation period. GC×GC-TOFMS results indicate that melanin could be one of the main products as a pigment. SEM images showed that melanin could localize on the conidia of A. carbonarius.

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Section: Resultsmentioning

confidence: 99%

Production of Bio-Based Pigments from Food Processing Industry By-Products (Apple, Pomegranate, Black Carrot, Red Beet Pulps) Using Aspergillus carbonarius

Arikan

Canlı

Caro³

et al. 2020

JoF

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“…Therefore, AAR and ADO are key enzymes for the biosynthesis of hydrocarbons. Thus, AAR and ADO have been applied for the production of biohydrocarbons in several organisms [10], including cyanobacteria [15–20], E. coli [11, 21–25], yeasts [26–28], chemoautotrophic eubacteria [29], and filamentous fungi [30]. In particular, ADO has been extensively used with other enzymes because ADO can catalyze a difficult and unusual reaction in the conversion of aldehydes to hydrocarbons [10, 23, 27, 31–35].…”

Section: Introductionmentioning

confidence: 99%

Identification of non-conserved residues essential for improving the hydrocarbon-producing activity of cyanobacterial aldehyde-deformylating oxygenase

Kudo

Hayashi

Arai

2019

Biotechnol Biofuels

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Background Cyanobacteria produce hydrocarbons corresponding to diesel fuels by means of aldehyde-deformylating oxygenase (ADO). ADO catalyzes a difficult and unusual reaction in the conversion of aldehydes to hydrocarbons and has been widely used for biofuel production in metabolic engineering; however, its activity is low. A comparison of the amino acid sequences of highly active and less active ADOs will elucidate non-conserved residues that are essential for improving the hydrocarbon-producing activity of ADOs. Results Here, we measured the activities of ADOs from 10 representative cyanobacterial strains by expressing each of them in Escherichia coli and quantifying the hydrocarbon yield and amount of soluble ADO. We demonstrated that the activity was highest for the ADO from Synechococcus elongatus PCC 7942 (7942ADO). In contrast, the ADO from Gloeobacter violaceus PCC 7421 (7421ADO) had low activity but yielded high amounts of soluble protein, resulting in a high production level of hydrocarbons. By introducing 37 single amino acid substitutions at the non-conserved residues of the less active ADO (7421ADO) to make its sequence more similar to that of the highly active ADO (7942ADO), we found 20 mutations that improved the activity of 7421ADO. In addition, 13 other mutations increased the amount of soluble ADO while maintaining more than 80% of wild-type activity. Correlation analysis showed a solubility-activity trade-off in ADO, in which activity was negatively correlated with solubility. Conclusions We succeeded in identifying non-conserved residues that are essential for improving ADO activity. Our results may be useful for generating combinatorial mutants of ADO that have both higher activity and higher amounts of the soluble protein in vivo, thereby producing higher yields of biohydrocarbons. Electronic supplementary material The online version of this article (10.1186/s13068-019-1409-8) contains supplementary material, which is available to authorized users.

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“…Overall propane yields were higher with this orthologue compared to the P. marinus ADO variant A134F [33]. Other studies have also described the production of alkanes from modifications of the FAS pathway, but they were limited to mostly medium and long chain hydrocarbons (min C5) [20,56,57].…”

Section: Heterologous Butyraldehyde Upregulation Via the Fatty Acid Bmentioning

confidence: 89%

Engineering nature for gaseous hydrocarbon production

2020

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The development of sustainable routes to the bio-manufacture of gaseous hydrocarbons will contribute widely to future energy needs. Their realisation would contribute towards minimising over-reliance on fossil fuels, improving air quality, reducing carbon footprints and enhancing overall energy security. Alkane gases (propane, butane and isobutane) are efficient and clean-burning fuels. They are established globally within the transportation industry and are used for domestic heating and cooking, non-greenhouse gas refrigerants and as aerosol propellants. As no natural biosynthetic routes to short chain alkanes have been discovered, de novo pathways have been engineered. These pathways incorporate one of two enzymes, either aldehyde deformylating oxygenase or fatty acid photodecarboxylase, to catalyse the final step that leads to gas formation. These new pathways are derived from established routes of fatty acid biosynthesis, reverse β-oxidation for butanol production, valine biosynthesis and amino acid degradation. Single-step production of alkane gases in vivo is also possible, where one recombinant biocatalyst can catalyse gas formation from exogenously supplied short-chain fatty acid precursors. This review explores current progress in bio-alkane gas production, and highlights the potential for implementation of scalable and sustainable commercial bioproduction hubs.

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Alkane biosynthesis by Aspergillus carbonarius ITEM 5010 through heterologous expression of Synechococcus elongatus acyl-ACP/CoA reductase and aldehyde deformylating oxygenase genes

Cited by 20 publications

References 29 publications

Production of Bio-Based Pigments from Food Processing Industry By-Products (Apple, Pomegranate, Black Carrot, Red Beet Pulps) Using Aspergillus carbonarius

Production of Bio-Based Pigments from Food Processing Industry By-Products (Apple, Pomegranate, Black Carrot, Red Beet Pulps) Using Aspergillus carbonarius

Identification of non-conserved residues essential for improving the hydrocarbon-producing activity of cyanobacterial aldehyde-deformylating oxygenase

Engineering nature for gaseous hydrocarbon production

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