Gas chromatographic determination of fatty acids contained in different lipid classes after their separation by solid-phase extraction

Giacometti, Jasminka; Milošević, Ana; Milin, Čedomila

doi:10.1016/s0021-9673(02)01159-7

Cited by 54 publications

(21 citation statements)

References 19 publications

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“…The approach we report is the application of simple solid phase extraction (SPE) cartridges (rather than TLC or ion exchange) to produce a few fractions from which lipids and classes of lipids can be characterized by rapid and direct MALDI-MS analysis. SPE approaches have been used for separation of different lipid classes in foods ( 21,22 ), oils ( 23 ), and biological tissues (23)(24)(25), most often after fatty acid methyl esterifi cation to facilitate GC separation. In this case, we report MALDI-MS analysis of the intact lipids.…”

Section: Lipid Standardsmentioning

confidence: 99%

A rapid separation technique for overcoming suppression of triacylglycerols by phosphatidylcholine using MALDI-TOF MS

Emerson

Gidden

Lay

et al. 2010

Journal of Lipid Research

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This article is available online at http://www.jlr.org where it is important to detect multiple lipid classes [i.e., phospholipids and triacylglycerols (TAGs)] include food analysis ( 1-3 ), cell biology ( 4 ), health effects ( 5 ), taxonomy ( 6 ), and other fi elds ( 7-11 ). However, because of different chemistries, it is often diffi cult to characterize one class of lipids in the presence of another, especially using rapid methods. Elegant methods have been developed for quantitative analysis in complex mixtures, but they are typically diffi cult to implement and are time consuming ( 8,12 ). There has been much recent interest in rapid MALDI-MS analysis of lipids due to its speed of analysis and high sensitivity ( 5,13,14 ). Sample preparation is quick because derivatization is not required (i.e., no silylation), HPLC separation is not needed, limited buffer or salt contamination is tolerated ( 9, 11, 13 ), and spectra are easy to interpret ( 13 ).MALDI-MS has functioned as both a qualitative and semiquantitative approach to measure and track effected biology-related phenomena. For example, Lay et al. ( 15 ) demonstrated a rapid method for the analysis of edible oils by MALDI-MS that allowed determination of the relative abundances of TAGs with suffi cient accuracy to correctly identify blind-coded samples of various oils. Gidden et al. ( 16 ) have also reported using MALDI-MS to rapidly differentiate Escherichia coli and Bacillus subtilis based on the phospholipid profi le and monitor changes in lipid content during the growth phases of the bacteria. However, this rapid MALDI-MS approach cannot currently be applied to experiments requiring the detection of multiple lipid classes in an unresolved mixture. Attempts to use direct MALDI-MS on such complex mixtures invariably results in entire classes of lipids being missed because of suppression effects.

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Section: Lipid Standardsmentioning

confidence: 99%

A rapid separation technique for overcoming suppression of triacylglycerols by phosphatidylcholine using MALDI-TOF MS

Emerson

Gidden

Lay

et al. 2010

Journal of Lipid Research

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show abstract

“…Solid-phase extraction (SPE) has been developed to isolate squalene from virgin olive oil without any chemical treatment. Rapidity, reliability, use of minimal amounts of solvent and automation are the main advantages of SPE in comparison to TLC and classical column chromatography (Giacometti et al, 2002). All the methods mentioned above are limited by small sample loading and using mixture of organic solvent in order to obtain good separations.…”

Section: Introductionmentioning

confidence: 99%

Design and operation of a modified silica gel column chromatography

Gunawan

Ismadji

2008

Journal of the Chinese Institute of Chemical Engineers

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“…These two techniques are robust and are quite economic in terms of time, labor and solvent consumption, compared to more conventional methods, and they can be automated [31].…”

Section: Extraction Of Fatty Acidsmentioning

confidence: 99%

Gas chromatography-mass spectrometry and high-performance liquid chromatography-tandem mass spectrometry in quantifying fatty acids

Wei

Zeng

2011

TrAC Trends in Analytical Chemistry

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Gas chromatographic determination of fatty acids contained in different lipid classes after their separation by solid-phase extraction

Cited by 54 publications

References 19 publications

A rapid separation technique for overcoming suppression of triacylglycerols by phosphatidylcholine using MALDI-TOF MS

A rapid separation technique for overcoming suppression of triacylglycerols by phosphatidylcholine using MALDI-TOF MS

Design and operation of a modified silica gel column chromatography

Gas chromatography-mass spectrometry and high-performance liquid chromatography-tandem mass spectrometry in quantifying fatty acids

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