Gas chromatography-mass spectrometry and high-performance liquid chromatography-tandem mass spectrometry in quantifying fatty acids

Wei, Gaoling; Zeng, Eddy Y.

doi:10.1016/j.trac.2011.05.005

Cited by 31 publications

(31 citation statements)

References 44 publications

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“…Individual 100 µM stock solutions of each internal standard (octanoic-d 15 6 A LTQ Orbitrap Elite mass spectrometer (ThermoFisher Scientific, Bremen, Germany) was operated in negative ionization mode and detection was performed over the mass range m/z 110-380 with a resolving power of 60'000 (at m/z = 400). The mass spectrometer was interfaced to the UPLC system using a HESI probe.…”

Section: Preparation Of Internal and External Standardsmentioning

confidence: 99%

High-Throughput Quantitative Lipidomics Analysis of Nonesterified Fatty Acids in Human Plasma

2016

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We present a high-throughput, nontargeted lipidomics approach using liquid chromatography coupled to high-resolution mass spectrometry for quantitative analysis of nonesterified fatty acids. We applied this method to screen a wide range of fatty acids from medium-chain to very long-chain (8 to 24 carbon atoms) in human plasma samples. The method enables us to chromatographically separate branched-chain species from their straight-chain isomers as well as separate biologically important ω-3 and ω-6 polyunsaturated fatty acids. We used 51 fatty acid species to demonstrate the quantitative capability of this method with quantification limits in the nanomolar range; however, this method is not limited only to these fatty acid species. High-throughput sample preparation was developed and carried out on a robotic platform that allows extraction of 96 samples simultaneously within 3 h. This high-throughput platform was used to assess the influence of different types of human plasma collection and preparation on the nonesterified fatty acid profile of healthy donors. Use of the anticoagulants EDTA and heparin has been compared with simple clotting, and only limited changes have been detected in most nonesterified fatty acid concentrations.

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Section: Preparation Of Internal and External Standardsmentioning

confidence: 99%

High-Throughput Quantitative Lipidomics Analysis of Nonesterified Fatty Acids in Human Plasma

2016

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“…Liquid chromatographic (LC) techniques with 2 ultraviolet detection and MS have also been used to identify and quantify FAMEs (e.g. Wei and Zeng 2011). Similarly, LC coupled with refractive index detection or MS (electrospray ionisation, atmospheric pressure chemical ionisation, atmospheric pressure photoionisation or matrix assisted laser desorption ionisation; ESI, APCI, APPI or MALDI) have been used for intact TAGS, as recently reviewed by Tena et al 2019.…”

Section: Introductionmentioning

confidence: 99%

Use of compound specific δ¹³C stable isotope analysis for intact triacylglycerides using high temperature gas chromatography up to 430°C

Sutton¹,

Tuthorn²,

Newton³

2019

Preprint

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“…Determination of fatty acid levels in food involves solvent extraction of the crude lipids, hydrolysis, purification, and derivatization to more volatile methyl esters for GC analysis (Bielawska et al 2010;RuizRodriguez et al 2010;Wei and Zeng 2011). Base-catalyzed and acid-catalyzed methylations have been utilized in preparing methyl ester derivatives; however, both techniques have drawbacks: (1) incomplete base-catalyzed methylation of Nacyl lipids and free fatty acids and (2) possible isomerization in acid-catalyzed reactions (Czauderna et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Determination of Fatty Acids in Beef by Liquid Chromatography-Electrospray Ionization Tandem Mass Spectrometry

et al. 2015

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A method was developed to separate, identify, and quantify 28 fatty acids of potential health relevance using liquid chromatography/electrospray ionizationmass spectrometry (LC/ESI-MS). Optimization of the experimental factors enabled baseline separation of the fatty acids including three pairs of closely related fatty acid isomers (C18:3n-3 and C18:3n-6; C18:1 and C18:1 t; and C20:3n-3 and C20:3n-6) that are challenging to separate. The limits of detection ranged from 0.01 to 0.26 mg L −1 for the 28 fatty acids, and average recovery (mean, n =4) was found to be 102 ±12 %. In addition, the proposed method was validated using a quality control standard mix of fatty acids which yielded acceptable precision and accuracy. Fatty acid concentrations in conventional grain-fed and organic grass-fed beef were determined, and the results show that grass-fed beef have a lower omega-6 to omega-3 ratio (1.6-2.8) compared to grain-fed beef (9.3-13.5). Principal component analysis (PCA) was applied on the resulting data to find correlations between significant fatty acid composition and the diet of beef samples.

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Gas chromatography-mass spectrometry and high-performance liquid chromatography-tandem mass spectrometry in quantifying fatty acids

Cited by 31 publications

References 44 publications

High-Throughput Quantitative Lipidomics Analysis of Nonesterified Fatty Acids in Human Plasma

High-Throughput Quantitative Lipidomics Analysis of Nonesterified Fatty Acids in Human Plasma

Use of compound specific δ¹³C stable isotope analysis for intact triacylglycerides using high temperature gas chromatography up to 430°C

Determination of Fatty Acids in Beef by Liquid Chromatography-Electrospray Ionization Tandem Mass Spectrometry

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