Gamma-Globulin Affinity for Normal Human Tissue of the Central Nervous System

Abstract

Immunofluorescent staining of the serum and cerebrospinal fluid of patients with and without neurological disease demonstrates an affinity of the gamma-globulin fraction for glial cells and myelin sheaths in normal human nervous tissue. This affinity appears to be specific for the 7S gamma-globulin fraction and is not seen with the other major protein fractions of serum and cerebrospinal fluid or with the fluorescein conjugated antisera alone.

Multiple sclerosis serum and cerebrospinal fluid immunoglobulin binding to fc receptors of oligodendrocytes

“…These studies have in turn led to two hypotheses. The hypothesis of Allerand and Yahr [6], which is supported by Aarli et a1 [l] and Traugott et a1 [35], is that IgG binding to CNS is immunologically nonspecific and is related to the affinity of nervous tissue, especially the myelin membrane, for gamma globulins. The hypothesis of Edgington and Dalessio [ 141, supported by Abramsky et a1 [3], is that IgG binding to components of CNS tissue is immunologically specific, is indicative of an antigen-antibody reaction, and is specific for MS.…”

“…At least a part of this synthesis has been interpreted as due to antibody synthesis within the CNS against an unknown MS antigen [32]. To test this hypothesis, experiments have been carried out to investigate the antibody nature of MS CSF to glia and myelin [ l , 3,6,14,17,35,381. These studies have in turn led to two hypotheses.…”

Detection of IgG on glioblastoma cell surfacein vivo

“…Secondly, the glioblastoma cells may also have IgG receptor sites on the surface membranes, as has been reported for glial cells and myelin sheaths in normal brain. It should be noted that 7 S gamma globulin has an affinity for the latter cell when examined by the immunofluorescence technique (Allerand et al 1964, Edginton et al 1970, Aarli et al 1975. The important fact brought out by this study is that IgG can cross the vascular capillary barrier in certain human glioblastomas.…”

Relationship of Age and Serum Immunoglobulins to Autoantibodies against Brain Constituents in Primates

“…The complement fixation test was the method applied in such studies by Wilkinson [17], the latex method by Fessel [8], the passive hemagglutination procedure by Tkach and Hokama [14], and the radioimmunoprécipitation test by Edgington and Dalessio [6]. Allerand and Yahr [1] and Edgington and Dalessio [6] emphasized that immunoglobulins (Jg) of the class IgG frequently yield positive tests also in the absence of autoallergic phenomena.…”

“…Among others, Diederichsen and Pyndt [4,5] evaluated neuronal cytoplasmic, Heath and Krupp [9] antinuclear, Allerand and Yahr [1] glia cell, Edgington and Dalessio [6] antimyelin antibodies, using principally the fluorescent antibody method. The complement fixation test was the method applied in such studies by Wilkinson [17], the latex method by Fessel [8], the passive hemagglutination procedure by Tkach and Hokama [14], and the radioimmunoprécipitation test by Edgington and Dalessio [6].…”