Galanthamine production by Leucojum aestivum in vitro systems

Pavlov, Atanas; Berkov, Strahil; Courot, Eric; Gocheva, Tatiana; Tuneva, Dimka; Pandova, B.; Georgiev, Milen I.; Georgiev, Vasil; Yanev, S.; Burrus, Monique; Илиева, М.

doi:10.1016/j.procbio.2006.12.006

Cited by 67 publications

(84 citation statements)

References 24 publications

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“…the galanthamine content in shoot-clumps was found to vary when the cultures were cultivated on nutrient media with different combinations of phytohormones (20). content + + of galanthamine also varied in shoot-clumps grown on a sole nutrient medium but induced from different wild populations (22) or calli (38). It was found that the light increases the galanthamine synthesis in a shoot-clump of L. aestivum cultivated in a liquid medium (38).…”

Section: Lycoris Radiatamentioning

confidence: 98%

“…however, the degree of cell and tissue differentiation, strongly influences the galanthamine content in these two species (38,44), and the lowest levels of galanthamine have been found in calli: 12 ug/g for L aestivum and 0.03 ug/g for N. confusus both referred to dry weight. As shoot-clump cultures have a higher ability to synthesise this compound, they have been used as a model system for studies on in vitro galanthamine production (8,9,38,44,45). the galanthamine content in shoot-clumps was found to vary when the cultures were cultivated on nutrient media with different combinations of phytohormones (20).…”

Section: Lycoris Radiatamentioning

confidence: 99%

“…content + + of galanthamine also varied in shoot-clumps grown on a sole nutrient medium but induced from different wild populations (22) or calli (38). It was found that the light increases the galanthamine synthesis in a shoot-clump of L. aestivum cultivated in a liquid medium (38). Galanthamine content in L. aestivum shoot-clumps ranges from traces to 0.5 mg/g of DW (22,38) and up to 2.5 mg per culture of N. confusus, of which 1.97 mg is released into the medium (8).…”

Section: Lycoris Radiatamentioning

confidence: 99%

“…It was found that the light increases the galanthamine synthesis in a shoot-clump of L. aestivum cultivated in a liquid medium (38). Galanthamine content in L. aestivum shoot-clumps ranges from traces to 0.5 mg/g of DW (22,38) and up to 2.5 mg per culture of N. confusus, of which 1.97 mg is released into the medium (8). The galanthamine proportion in alkaloid mixtures of L. aestivum shoot-clumps varies greatly among different lines, sometimes consisting almost 100 %.…”

Section: Lycoris Radiatamentioning

confidence: 99%

“…Due to the increased demand for galanthamine and the limited availability of plant sources, in vitro culture of galanthamine-producing species has attracted the attention of researchers as an alternative approach for its sustainable production (17,38).…”

Section: Introductionmentioning

confidence: 99%

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Plant Sources of Galanthamine: Phytochemical and Biotechnological Aspects

Berkov

Georgieva

Kondakova

et al. 2009

Biotechnology & Biotechnological Equipment

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Section: Lycoris Radiatamentioning

confidence: 98%

Section: Lycoris Radiatamentioning

confidence: 99%

Section: Lycoris Radiatamentioning

confidence: 99%

Section: Lycoris Radiatamentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Plant Sources of Galanthamine: Phytochemical and Biotechnological Aspects

Berkov

Georgieva

Kondakova

et al. 2009

Biotechnology & Biotechnological Equipment

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Elicitation of galanthamine production by leucojum aestivum shoots grown in temporary immersion system

Schumann¹,

Torras-Claveria

Berkov

et al. 2013

Biotechnology Progress

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The influence of different elicitors (copper sulfate, silver nitrate, salicylic acid and methyl jasmonate), on both the growth and alkaloid production of Leucojum aestivum shoots grown in a temporary immersion system was studied. Seven Amaryllidaceae alkaloids and three protoalkaloids were quantitatively determined by GC-MS analysis in leaves and bulblets, separately. Methyl jasmonate was found to significantly improve the production of galanthamine (GAL) in both leaves and bulblets. The content of GAL released to the liquid nutrient medium was also measured. The release of GAL into the liquid medium took place mainly in the first 2 weeks determined by harvesting the liquid nutrient medium after 2 weeks and measuring the GAL content (1st subculturing step).

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Galanthamine production by Leucojum aestivumL. shoot culture in a modified bubble column bioreactor with internal sections

Georgiev

Ivanov

Berkov

et al. 2012

Engineering in Life Sciences

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Shoot culture of summer snowflake (Leucojum aestivum L.) was successfully cultivated in an advanced modified glass‐column bioreactor with internal sections for production of Amaryllidaceae alkaloids. The highest amounts of dry biomass (20.8 g/L) and galanthamine (1.7 mg/L) were achieved when shoots were cultured at 22°C and 18 L/(L·h) flow rate of inlet air. At these conditions, the L. aestivum shoot culture possessed mixotrophic‐type nutrition, synthesizing the highest amounts of chlorophyll (0.24 mg/g DW (dry weight) chlorophyll A and 0.13 mg/g DW chlorophyll B). The alkaloids extract of shoot biomass showed high acetylcholinesterase inhibitory activity (IC50 = 4.6 mg). The gas chromatography–mass spectrometry (GC/MS) profiling of biosynthesized alkaloids revealed that galanthamine and related compounds were presented in higher extracellular proportions while lycorine and hemanthamine‐type compounds had higher intracellular proportions. The developed modified bubble‐column bioreactor with internal sections provided conditions ensuring the growth and galanthamine production by L. aestivum shoot culture.

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Galanthamine production by Leucojum aestivum in vitro systems

Cited by 67 publications

References 24 publications

Plant Sources of Galanthamine: Phytochemical and Biotechnological Aspects

Plant Sources of Galanthamine: Phytochemical and Biotechnological Aspects

Elicitation of galanthamine production by leucojum aestivum shoots grown in temporary immersion system

Galanthamine production by Leucojum aestivumL. shoot culture in a modified bubble column bioreactor with internal sections

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