Elicitation of galanthamine production by <i>leucojum aestivum</i> shoots grown in temporary immersion system

Schumann, Anika; Torras-Claveria, Laura; Berkov, Strahil; Claus, Diana; Gerth, André; Codina, Carles

doi:10.1002/btpr.1677

Cited by 19 publications

(3 citation statements)

References 20 publications

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“…radiata); 53,54 the first two methods have been suffering from technical problems, so the in vitro culture technique can be an alternative source of galanthamine. 55 Scholars have carried out many tests about the effectors of in vivo culture in L. aestivum, such as mineral nutrients, 56 carbohydrate sources, 57,58 auxins, 59 cytokinins, 60 ethylene and melatonin, [61][62][63] and physical factors (temperature and medium) on alkaloid synthesis, 64 in which Ptak's team has made a remarkable contribution.In addition, studies on the induction of methyl jasmonate and salicylic acid [65][66][67] and the use of labeling to determine the biotransformation of the precursor 4′-O-methylnorbelladine have also been reported. [68][69][70] L. aestivum in vitro culture has been improving to aid production; the first document about temporary soaking measures in stem segment culture was from Ivanov, and the result showed that the highest production of galanthamine was achieved by soaking at a frequency of 15min at 26°C.…”

Section: Keyword Co-occurrence Analysismentioning

confidence: 99%

Research trend analysis of Leucojum aestivum based on Citespace visualization software

Wang¹,

Tang²,

Zhou³

et al. 2022

HIJ

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The secondary metabolite of Leucojum aestivum lycorine, is of high medicinal value and has attracted much attention internationally. Therefore, it is essential to review and analyze the existing literature on L. aestivum to clarify the research progress, research bottlenecks, and applications of lycorine in landscapes. In this study, we used the Web of Science (WOS) and CNKI Chinese journal database as data sources to analyze the bibliometric analysis of L. aestivum-related literature in terms of the number of publications, journal sources, research institutions, keywords, and co-cited literature using Citespace visualization software. The results showed that the number of L. aestivum-related studies decreased compared to previous years, but the number of publications showed a high growth trend in this research frontier. Bulgaria has a significant dominance in L. aestivum-related research, while China has fewer publications in this field but focuses more on the frontiers of the discipline. The frontiers of L. aestivum research concentrate on improving in vitro culture devices, changing plant culture strategies, and adding chemical additives to improve the yield of compounds in plants; new methods for lycorine synthesis; identification of ornamental plant species and detection of compounds by electrochemical methods; practical applications of lycorine in medicine and its mechanism of action. It is inferred that future research will focus on the ornamental value, medicinal value, cultivation methods, and innovation of lycorine synthesis methods of aestivum.

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Section: Keyword Co-occurrence Analysismentioning

confidence: 99%

Research trend analysis of Leucojum aestivum based on Citespace visualization software

Wang¹,

Tang²,

Zhou³

et al. 2022

HIJ

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“…The combination of elicitor (Chitoplant ® ) and TIS (two vessels bioreactor), as a reliable alternative for the genetic modification, increased the cardenolides production 2.2-fold in Digitalis lanata shoots [122], significantly improved galanthamine content in the leaves and bulblets of Leucojum aestivum [126], and stimulated the production of thapsigargin and nortrilobolide in vitro Thapsia garganica shoots [137]. Moreover, the elicitation induced the production and accumulation of phenylpropanoid metabolites in both fresh biomass and culture medium of Aristotelia chilensis [49].…”

Section: Medium Composition and Bioactive Compounds Contentmentioning

confidence: 99%

Temporary Immersion System for Production of Biomass and Bioactive Compounds from Medicinal Plants

et al. 2021

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The cultivation of medicinal plants and the production of bioactive compounds derived from them are of fundamental importance and interest, not only at the pharmacological level but also in nutraceutical and cosmetic industries and in functional foods, as well as plant protection in agriculture. In order to respond adequately to the increased demands of the global market from a quantitative and qualitative point of view and to guarantee environmental sustainability of the productions, it is necessary to resort to innovation tools, such as tissue culture in vitro technology. Nowadays, it is well known that the cultivation through the Temporary Immersion System (TIS) in a bioreactor has considerable advantages both for the in vitro mass production of the plants and for the production of secondary metabolites. The present review focuses on the application of TIS during the last two decades to produce biomass and bioactive compounds from medicinal plants. Indeed, almost one hundred papers are discussed, and they particularly focus on the effects of the culture system, vessel design and equipment, immersion time and frequency, and substrate composition for 88 medicinal species in TIS bioreactor culture.

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“…Consequently, alternative approaches have been explored by researchers to ensure its sustainable production ( Pavlov et al, 2007 ; Ferdausi et al, 2019 ). For example, in vitro cultures have been used to effectively produce Gal ( Ivanov et al, 2013 ; Schumann et al, 2013 ; Berkov et al, 2014 ; Saliba et al, 2015 ). In addition, various elicitors have been used to increase secondary metabolite levels ( Jimenez-Garcia et al, 2013 ; Ji et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Transcriptomic and Metabolomic Analyses Reveals That Exogenous Methyl Jasmonate Regulates Galanthamine Biosynthesis in Lycoris longituba Seedlings

Zheng

et al. 2021

Front. Plant Sci.

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The Amaryllidaceae alkaloid galanthamine (Gal) in Lycoris longituba is a secondary metabolite that has been used to treat Alzheimer’s disease. Plant secondary metabolism is affected by methyl jasmonate (MeJA) exposure, although the regulatory mechanisms of MeJA on L. longituba seedlings remains largely unknown. In the present study, 75, 150, and 300 μM MeJA were used as treatments on L. longituba seedlings for 7, 14, 21, and 28 days, while 0 μM MeJA was used as the control (MJ-0). The effect of exogenous MeJA on Gal synthesis in L. longituba was then investigated using transcriptomic sequencing and metabolite profiling via GC-MS and LC-MS analysis. Galanthamine (Gal), lycorine (Lyc), and lycoramine (Lycm) abundances were 2. 71-, 2. 01-, and 2.85-fold higher in 75 μM MeJA (MJ-75) treatment plants compared to MJ-0 treatment plants after 7 days of cultivation. Transcriptomic analysis further showed that MJ-75 treatment significantly induced the expression of norbelladine synthase (NBS) and norbelladine 4′-O-methyltransferase (OMT), which are involved in the Gal biosynthesis pathway. In addition, increased expression was observed in MJ-75 treatment plants for genes in the JA synthesis and JA signaling pathways including those of allene oxide cyclase (AOC), 12-oxo-phytodienoic acid reductase (OPR), jasmonic acid amino acid synthase (JAR), and transcription factor MYC. The L. longituba tyrosine decarboxylase (LlTYDC) enzyme was identified and proposed to be involved in the Gal biosynthetic pathway. Metabolomics results demonstrated that the accumulation of Amaryllidaceae alkaloids, and especially alkaloids in the Gal biosynthesis pathway, could be induced by MJ-75 treatment. Interestingly, metabolites in the JA synthesis pathway were also affected by MeJA treatment. Overall, this multi-omics study suggests that both the JA synthesis/JA signaling and Gal biosynthesis pathways were affected by exogenous MeJA treatment. This comprehensive study of gene expression and metabolite contents can help us better understand the molecular mechanisms underlying MeJA-mediated Gal biosynthesis in L. longituba.

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Elicitation of galanthamine production by leucojum aestivum shoots grown in temporary immersion system

Cited by 19 publications

References 20 publications

Research trend analysis of Leucojum aestivum based on Citespace visualization software

Research trend analysis of Leucojum aestivum based on Citespace visualization software

Temporary Immersion System for Production of Biomass and Bioactive Compounds from Medicinal Plants

Transcriptomic and Metabolomic Analyses Reveals That Exogenous Methyl Jasmonate Regulates Galanthamine Biosynthesis in Lycoris longituba Seedlings

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