Galacto-oligosaccharide production from lactose by an enzymic batch reaction using β-galactosidase

Iwasaki, Kenichi; Nakajima, Mitsutoshi; Nakao, Shinichi

doi:10.1016/0032-9592(94)00067-0

Cited by 108 publications

(76 citation statements)

References 2 publications

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“…The chemical products used (PRS quality) are glucose, citric acid, K 2 HPO 4 , KCl, trichloroacetic acid (supplied [24] Di-, tri-and tetra-saccharides formation [34] by Panreac), MgCl 2 ·6H 2 O (Prolabo), monohydrate lactose (Scharlau), and galactose (Across).…”

Section: Methodsmentioning

confidence: 99%

A new kinetic model proposed for enzymatic hydrolysis of lactose by a β-galactosidase from Kluyveromyces fragilis

Jurado

Camacho

Luzón

et al. 2002

Enzyme and Microbial Technology

101

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We study the enzymatic hydrolysis of lactose by a commercial enzyme from a selected strain of Kluyveromyces fragilis. The variables analyzed were: temperature (25-40 • C), enzyme concentration (0.1-3.0 g l −1 ), lactose concentration (0.0278-0.208 M), and initial galactose concentration (0.0347 M). On the basis of the data analyzed, both published and in the present work, we propose a Michaelis-Menten kinetic model with inhibition by the product (galactose), which reveals that the substrate (lactose) and the product (galactose) present similar affinity for the active site of the enzyme.

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Section: Methodsmentioning

confidence: 99%

A new kinetic model proposed for enzymatic hydrolysis of lactose by a β-galactosidase from Kluyveromyces fragilis

Jurado

Camacho

Luzón

et al. 2002

Enzyme and Microbial Technology

101

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show abstract

“…Compared to lactose concentration and lactose conversion, other process parameters, such as enzyme concentration, pH and temperature, have minimal effects on GOS production (Iwasaki et al 1996), although they affect the reaction rates. The results of the study led us to the conclusion that cell free extract of S. thermophilus is a food grade source of β-galactosidase, having appreciable transgalactosylation activity.…”

Section: Discussionmentioning

confidence: 99%

Production of β-galactosidase from streptococcus thermophilus for galactooligosaccharides synthesis

et al. 2014

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Efficiency of different methods for disruption of Streptococcus thermophilus cells, isolated from different dairy products, to release β-galactosidase and synthesis of GOS by extracted enzyme using whey supplemented with different concentrations of lactose as a substrate was studied. Unlike most other studies on GOS synthesis which used only one method of cell disruption and only few microbial strains, we compared five different cell disruption methods and used 30 strains of S. thermophilus in order to find out the most effective method and efficient strain for production of β-galactosidase. Appreciable amount of GOS (53.45 gL −1 ) was synthesized at a lactose concentration of 30 %, using enzyme (10 U mL −1 of reaction medium), extracted from S. thermophilus within a very short incubation time of 5 h at a temperature of 40°C and pH 6.8. S. thermophilus is heavily employed in the preparation of fermented dairy products but this study extends the use of this organism for the production of GOS, a potential prebiotic.

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“…According to some studies, GOS synthesis by BGases is a dynamic equilibrium-controlled process (10). Thus, most attempts to improve GOS yields have focused on controlling the kinetics of the transglycosylation reaction using high concentrations of lactose, high temperature, or low water concentrations (6). To reduce the amount of water, oligosaccharide synthesis was performed in organic solvents, which shifted the reaction equilibrium to GOS synthesis (2).…”

Section: Discussionmentioning

confidence: 99%

Cell Surface Engineering of a β-Galactosidase for Galactooligosaccharide Synthesis

Wang

et al. 2009

Appl Environ Microbiol

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Galacto-oligosaccharide production from lactose by an enzymic batch reaction using β-galactosidase

Cited by 108 publications

References 2 publications

A new kinetic model proposed for enzymatic hydrolysis of lactose by a β-galactosidase from Kluyveromyces fragilis

A new kinetic model proposed for enzymatic hydrolysis of lactose by a β-galactosidase from Kluyveromyces fragilis

Production of β-galactosidase from streptococcus thermophilus for galactooligosaccharides synthesis

Cell Surface Engineering of a β-Galactosidase for Galactooligosaccharide Synthesis

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