Cell Surface Engineering of a β-Galactosidase for Galactooligosaccharide Synthesis

Li, Yumei; Lu, Lili; Wang, Hongmei; Xu, Xiaodong; Xiao, Min

doi:10.1128/aem.00326-09

Cited by 19 publications

(9 citation statements)

References 22 publications

(20 reference statements)

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“…HAB, hydrophilic acrylic beads (Eupergit C ® ); mPOS‐PVA, magnetic polysiloxane‐polyvinyl alcohol; mPOS–PANI, magnetic polysiloxane–polyaniline; PVC‐film, polyvinyl chloride film; AER, anion exchange resin; ATPS, aqueous 2‐phase systems; RM, dioctyl sodium sulfosuccinate/isooctane reverse micelles; MA, recycled membrane‐assisted system; CMAR, membrane‐assisted reactor (continuous); PB, packed bed assisted system; PBR, packed bed reactor (continuous); MW, microwave power 12 watt. a, Del‐Val and others (2001), Del‐Val and Otero (2003), Aslan and Tanriseven (2007), Cardelle‐Cobas and others (2008); b, Del‐Val and Otero (2003); c, Aslan and Tanriseven (2007); d, Toba and Adachi (1978), Prenosil and others (1987); e, Prenosil and others (1987), Iwasaki and others (1996), Stevenson and others (1996), Boon and others (2000b), Chen and others (2001), Cheng and others (2006a), Gaur and others (2006), Matella and others (2006), Neri and others (2009a); f, Chen and others (2001); g, Matella and others (2006); h, Czermak and others (2004), Ebrahimi and others (2006b); i, Prenosil and others (1987), Rustom and others (1998); j, Sheu and others (1998), Gaur and others (2006); k, Albayrak and Yang (2002a, 2002b, 2002c), Matella and others (2006); l, Matella and others (2006); m, Albayrak and Yang (2002b); n, Neri and others (2009a); o, Neri and others (2009b); p, Leiva and Guzman (1995); q, Li and others (2008); r, Li and others (2009); s, Shin and Yang (1996); t, Cruz and others (1999); u, Nakkharat and Haltrich (2007); v, Nakkharat and Haltrich (2006, 2007); w, Nakkharat and Haltrich (2007); x, Prakash and others (1987); y, Burvall and others (1979), Boon and others (2000a), Kim and others (2001), Chockchaisawasdee and others (2005), Cheng and others (2006a), Martinez‐Villaluenga and others (2008); z, Czermak and others (2004), Ebrahimi and others (2006a), Engel and others (2008); aa, Maugard and others (2003); ab, Maugard and others (2003); ac, Zhou and others (2003); ad, Rustom and others (1998); Foda and Lopez‐Leiva (2000); ae,...…”

Section: Gos Productionmentioning

confidence: 96%

“…These researchers concluded that these improved GOS yields result from the fact that the glucose is consumed for cell growth, since glucose has been found to inhibit GOS synthesis. More recently, an innovative strategy for GOS synthesis by anchoring β‐galactosidase from P. expansum on the cell surface of S. cerevisiae as an immobilized enzyme was also successfully applied (Li and others 2009).…”

Section: Gos Productionmentioning

confidence: 99%

“…( Cheng and others 2006c ); 10, B. circulans (β‐GalII) ( Mozaffar and others 1984 ); 11, B. circulans ( Boon and others 1999 ); 12, Bacillus sp. ( Cheng and others 2006a ); 13, G. stearothermophilus ( Placier and others 2009 ); 14, C. saccharolyticum ( Stevenson and others 1996 ); 15, L. acidophilus ( Nguyen and others 2007 ); 16, L. reuteri ( Splechtna and others 2006 ); 17, S. thermophilus ( Greenberg and Mahoney 1983 ); 18, E. coli ( Huber and others 1976 ); 19, T. maritima ( Ji and others 2005 ); FUNGI: 20, A. aculeatus ( Cardelle‐Cobas and others 2008 ); 21, A. niger ( Toba and Adachi 1978 ); 22, A. oryzae ( Iwasaki and others 1996 ); 23, P. expansum ( Li and others 2008 ); 24, P. expansum (β‐galactosidase gene from P. expansum expressed on the cell surface of S. cerevisiae ) ( Li and others 2009 ); 25, P. funiculosum ( Shin and Yang 1996 ); 26, P. simplicissimum ( Cruz and others 1999 ); 27, T. thermophilus ( Nakkharat and Haltrich 2007 ); 28, K. lactis ( Cheng and others 2006b ); 29, K. marxianus ( Roberts and Pettinati 1957 ); 30, S. magnum ( Onishi and Tanaka 1997 ); 31, S. magnum ( Onishi and others 1996 ); 32, S. elviae ( Onishi and Tanaka 1995 ); 33, S. elviae ( Onishi and others 1995 ); 34, R. minuta ( Onishi and Tanaka 1996 ); 35, R. minuta ( Onishi and Yokozeki 1996 ); 36, S. singularis ( Shin and others 1998 ); 37, S. singularis ( Sakai and others 2008 ).…”

Section: Gos Productionmentioning

confidence: 99%

See 2 more Smart Citations

Galacto‐Oligosaccharides: Production, Properties, Applications, and Significance as Prebiotics

Torres¹,

Gonçalves²,

Teixeira³

et al. 2010

Comp Rev Food Sci Food Safe

519

394

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Galacto-oligosaccharides (GOS) have now been definitely established as prebiotic ingredients after in vitro and animal and human in vivo studies. Currently, GOS are produced by glycoside hydrolases (GH) using lactose as substrate. Converting lactose into GOS by GH results in mixtures containing GOS of different degrees of polymerization (DP), unreacted lactose, and monomeric sugars (glucose and galactose). Recent and future developments in the production of GOS aim at delivering purer and more efficient mixtures. To produce high-GOS-content mixtures, GH should not only have good ability to catalyze the transgalactosylation reaction relative to hydrolysis, but also have low affinity for the GOS formed relative to the affinity for lactose. In this article, several microbial GH, proposed for the synthesis of GOS, are hierarchized according to the referred performance indicators. In addition, strategies for process improvement are discussed. Besides the differences in purity of GOS mixtures, differences in the position of the glycosidic linkages occur, because different enzymes have different regiochemical selectivity. Depending on oligosaccharide composition, GOS products will vary in terms of prebiotic activity, as well as other physiological effects. This review focuses on GOS production from synthesis to purification processes. Physicochemical characteristics, physiological effects, and applications of these prebiotic ingredients are summarized. Regulatory aspects of GOS-containing food products are also highlighted with emphasis on the current process of health claims evaluation in Europe.

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Section: Gos Productionmentioning

confidence: 96%

Section: Gos Productionmentioning

confidence: 99%

Section: Gos Productionmentioning

confidence: 99%

See 1 more Smart Citation

Galacto‐Oligosaccharides: Production, Properties, Applications, and Significance as Prebiotics

Torres¹,

Gonçalves²,

Teixeira³

et al. 2010

Comp Rev Food Sci Food Safe

519

394

View full text Add to dashboard Cite

show abstract

“…The β‐galactosidase anchored yeast could directly utilize lactose to produce GOS, as well as the by‐products glucose and a small quantity of galactose. The glucose was consumed by the yeast, and the galactose was used for β‐galactosidase expression, thus greatly facilitating GOS synthesis (Li and others 2009). …”

Section: Biotechnological Methods For Enhanced Gos Productionmentioning

confidence: 99%

Galactooligosaccharides: Novel Components of Designer Foods

Sangwan¹,

Tomar²,

Singh³

et al. 2011

Journal of Food Science

196

149

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Since introduction of functional foods, commercialization of the traditionally used probiotics has ushered in more followers into the new fraternity of sophisticated, health-conscious consumers. In 1995, this was followed by the first introduction of prebiotics. Prebiotics are defined as "a non-digestible feed supplement, beneficially affecting the host by selectively stimulating growth and/or activity in one or a limited number of bacteria in the colon." The number of new product introductions with prebiotics has steeply increased over the last few years. Paradoxically, probiotics have limited applications as these cannot be used in wide range of food products because of their viability issue. Fortunately, prebiotics do not suffer from any such constraint and can be used in a wide range of food products. Probiotics do not have a long shelf life in their active form. In most cases, refrigeration is required to maintain the shelf life. While probiotics are predominantly used in fermented dairy products, the use of prebiotics has expanded into other food categories. Prebiotics have successfully been incorporated in a wide variety of human food products such as baked goods, sweeteners, yoghurts, nutrition bars, and meal replacement shakes. For instance, the introduction of galacto-oligosaccharides (GOS) into baby foods has been very successful. GOS, which are identical to the human milk oligosaccharides, has emerged with strong clinical support for both digestive and immune health. Various aspects related to GOS such as types and functions of functional food constituents with special reference to GOS, their role as prebiotics, and enhanced industrial production through microbial intervention are dealt in this review.

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“…This surface engineered yeast is able to utilize starch as the sole carbon source directly and produce the expected CD products as well as glucose and maltose. This application was later investigated in another system [7].…”

Section: Introductionmentioning

confidence: 97%

The effect of cyclodextrins on the ethanol tolerance of microorganisms suggests potential application

Liang

Wang

Gao

et al. 2011

J Ind Microbiol Biotechnol

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Cyclodextrins (CDs) are used in food, pharmaceutical, and chemical industries, as well as agriculture and environmental engineering. Cyclodextrin glucanotransferase (CGTase) is an important industrial extracellular enzyme which is used to produce CDs and oligosaccharides. We previously developed a novel yeast-surface CGTase expression system which was used for the production of CDs from starch. In the present study, we showed that the presence of CDs may increase the ethanol tolerance of microorganisms. The cell numbers of Saccharomyces cerevisiae and Escherichia coli in the presence of β-cyclodextrin and ethanol were 1,000-fold and 10-fold higher than that without CDs. The yeast strain with the immobilized CGTase produced 13 g CDs/l and 1.8 g ethanol/l when it was incubated in yeast medium supplemented with 4% starch. The effect of CDs on microorganisms suggests a potential application for the co-production of CDs and ethanol.

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Cell Surface Engineering of a β-Galactosidase for Galactooligosaccharide Synthesis

Cited by 19 publications

References 22 publications

Galacto‐Oligosaccharides: Production, Properties, Applications, and Significance as Prebiotics

Galacto‐Oligosaccharides: Production, Properties, Applications, and Significance as Prebiotics

Galactooligosaccharides: Novel Components of Designer Foods

The effect of cyclodextrins on the ethanol tolerance of microorganisms suggests potential application

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