Production of β-galactosidase from streptococcus thermophilus for galactooligosaccharides synthesis

Abstract

Efficiency of different methods for disruption of Streptococcus thermophilus cells, isolated from different dairy products, to release β-galactosidase and synthesis of GOS by extracted enzyme using whey supplemented with different concentrations of lactose as a substrate was studied. Unlike most other studies on GOS synthesis which used only one method of cell disruption and only few microbial strains, we compared five different cell disruption methods and used 30 strains of S. thermophilus in order to find out the most effective method and efficient strain for production of β-galactosidase. Appreciable amount of GOS (53.45 gL −1 ) was synthesized at a lactose concentration of 30 %, using enzyme (10 U mL −1 of reaction medium), extracted from S. thermophilus within a very short incubation time of 5 h at a temperature of 40°C and pH 6.8. S. thermophilus is heavily employed in the preparation of fermented dairy products but this study extends the use of this organism for the production of GOS, a potential prebiotic.

Whey-derived valuable products obtained by microbial fermentation

“…Ranjana et al (2011) proposed a method for using lactose from whey to produce GOS by using the β-galactosidase from Bacillus circulans in batch reactor and in recycle membrane reactor; the authors observed better results with the latter method (33 % higher production) obtaining 77 % of pure GOS. Sangwan et al (2014) reported that the S. thermophilus β-galactosidase could produce GOS in an appreciable amount (53.45 g/L) using lactose-supplemented whey as substrate. This amount was obtained using a total of 30 % lactose concentration and 10 U/mL of enzyme after 5 h at 40°C and pH 6.8.…”

Galactooligosaccharides reduce infection caused by Listeria monocytogenes and modulate IgG and IgA levels in mice

“…GOS was prepared in our laboratory using bgalactosidase extracted from native strain of S. thermophilus (Sangwan, Tomar, Ali, Singh, & Singh, 2014). Whey supplemented with lactose was used as a cost effective substrate for the production.…”

Synthesis of Galactooligosaccharides in Milk and Whey: A Review

“…While Geiger et al. () reported a yield of 34%, Sangwan, Tomar, Ali, Singh, and Singh () detected no GOS formation at all (Table , no. 86 and 87).…”

“…References: a: Prenosil et al, ; b: Fischer & Kleinschmidt, ; c: Mause, ; d: Jovanovic‐Malinovska, Fernandes, Winkelhausen, & Fonseca, ; e: Valero, ; f: Rodriguez‐Colinas et al, ; g: Gosling et al, ; h: Rodriguez‐Colinas, Poveda, Jimenez‐Barbero, Ballesteros, & Plou, ; i: Cho et al, ; j: Hellerová & Čurda, ; k: Pocedičová et al, ; l: Splechtna et al, ; m: Vasiljevic & Jelen, ; n: Lopez Leiva & Guzman, ; o: Wierzbicki & Kosikowski, ; p: Sen, Nath, Bhattacharjee, Chowdhury, & Bhattacharya, ; q: Sen, Bhattacharjee, & Bhattacharya, ; r: Geiger et al, ; s: Rustom, Foda, & López‐Leiva, ; t: Hugo, Bruno, & Golowczyc, ; u: Golowczyc et al, ; v: Sabater, Olano, Prodanov, Montilla, & Corzo, ; w: Manucci, ; x: Adamczak, Charubin, & Bednarski, ; y: Foda & Lopez‐Leiva, ; z: Padilla et al, ; aa: Sangwan et al., ; ab: Lisboa et al., ; ac: Song et al., ; ad: Čurda, Rudolfová, Štětina, & Dryák, ; ae: Frenzel et al., ; af: Chen et al., ; ag: Zerge, ; ah: Lorenzen et al., ; ai: Pruksasri & Supee, ; aj: Rodriguez‐Colinas et al., ; ak: Mozaffar et al., ; al: Bakken, Hill, & Amundson, ; am: Foda, Kholif, & Kholif, ; an: Burvall et al., ; ao: Greenberg & Mahoney, ; ap: Roy et al., ; aq: Yadav et al., , ar: Liu et al., , as: Lisboa et al., , at: Roberts & Pettinati, , au: Ruiz‐Matute et al., , av: Patent No. EP 0 323 201 B1, 1990, aw: Goulas et al., .…”