Gaharu Leaf Extract Water Reduce MDA and 8-OHdG Levels and Increase Activities SOD and Catalase in Wistar Rats Provided Maximum Physical Activity

Parwata, I Made Oka Adi; Manuaba, Ida Bagus Putra; Sutirtayasa, I Wayan Putu; Wita, I Wayan

doi:10.15562/bmj.v5i3.297

Cited by 5 publications

(4 citation statements)

References 2 publications

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“…In the nanoextract of kecombrang flowers, a yellow-orange color was obtained with 10% NaOH, with Bate Smith-Metcalfe and Wilstater reagents yellow. From these results, it can be seen that the most obvious intensity of color change is produced by the nano extract of kecombrang flowers, so it can be said to have a higher flavonoid content than the extract of kecombrang flowers [10].…”

Section: Phytochemical Testmentioning

confidence: 97%

“…The NaTPP polyanion is formed because it dissociates in water releasing sodium ions and tripolyphosphate ions which then react with NH 3+ from chitosan. NaTPP salt is used to function as a cross-linking agent, because this material tends to have non-toxic properties, so this additional material has been approved by the United States Food and Drug Administration [10]. The addition of the NaTPP solution drop by drop accompanied by stirring using a magnetic stirrer aims to prevent solidification so that the resulting nanoparticles do not form large lumps, as well as to obtain a constant stirring speed [13].…”

Section: Kecombrang Flowers Extract Nanoparticlesmentioning

confidence: 99%

“…The ionic gelation method is one of the methods applied to encapsulate extracts into nanoparticles using a cross-linking mechanism between chitosan using polyanion salts such as NaTPP. Kecombrang flowers extract can have a negative charge in water because the hydroxyl groups of flavonoid compounds can hold ionic interactions with the positively charged amine groups of chitosan [9], and the addition of NaTPP which functions as a crosslinker agent to stabilize the nanoparticles formed [10].…”

Section: Introductionmentioning

confidence: 99%

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Encapsulation of Kecombrang (Etlingera elatior) Flowers Extract Using the Ionic Gelation Method

Wahjuni,

Bogoriani,

Puspawati

et al. 2024

IJDNE

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The extract flower of Etlingera elatior (kecombrang) is one of herb interested for research. This flower known to have a beneficial antioxidant activity. This antioxidant effect was obtained as a results of high flavonoid content. Photosensitivity and low bioavailability and their fast metabolism were the main limitations for its use, which can be overcome through encapsulation of the extract (flavonoids) into nanoparticle-based chitosan-tripolyphosphate by ionic gelation method. This is an observational study aims to encapsulate kecombrang flowers extract that is already form in nanoextract and assess the characterization. The nanoparticle extract of this flower obtained has a particle size of 312.7 nm with a zeta potential of -16.2 mV and a polydispersity index value of 0.502, amorphous (non-uniform) morphology, has a tendency to be spherical, and has an imperfectly spherical shape, has functional groups -OH, -NH, C=O, and a phosphate group. Overall Kecombrang flowers extract is able to be encapsulated in chitosantripolyphosphate.

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Section: Phytochemical Testmentioning

confidence: 97%

Section: Kecombrang Flowers Extract Nanoparticlesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Encapsulation of Kecombrang (Etlingera elatior) Flowers Extract Using the Ionic Gelation Method

Wahjuni,

Bogoriani,

Puspawati

et al. 2024

IJDNE

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“…In this process, MDA is a marker that directly describes the intensity of lipid peroxidation. [21][22][23]…”

Section: Original Articlementioning

confidence: 99%

The oxidative-stress level determine patientâ€™s outcomes with a severe head injury at Sanglah General Hospital, Denpasar, Indonesia

Wirawan¹,

Golden²,

Niryana³

2019

Intisari Sains Medis

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Background: In secondary brain injury, oxidative stress will occur due to a balance disorder between pro-oxidants with antioxidants. The antioxidant activity that is often used to assess oxidative stress, such as malondialdehyde (MDA), is superoxide dismutase (SOD) and glutathione peroxidase (GPx). This study aims to evaluate the level of oxidative stress, reflected by the MDA serum level and SOD level to determine the outcomes of patients with severe head injury. Method: A cross-sectional analytic study was conducted among 40 patients with severe head injury within 24 hours post-trauma at Emergency Ward, Surgery Department, Sanglah General Hospital Denpasar from January-June 2017. MDA and SOD levels were assessed using ELISA at Clinical Pathology Laboratory, Sanglah General Hospital. Data were analyzed using SPSS ver. 16 software. Results: This study found significant differences on elevated MDA levels (p-value < 0.05) in patients who died or had a persistent vegetative state, patients with severe disability and those with a good recovery/moderate disability. The statistical analysis also found a significant difference in MDA serum levels among patient with severe disability and patients with a good recovery/moderate disability (P=0,028). Meanwhile, there was no significant correlation between SOD serum levels and patients outcome (P>0.05). Conclusion: Increased MDA serum levels is a significant factor in predicting outcomes of patients with severe head injury.

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Phytochemical Screening and Antioxidant Activity of Hippobroma longiflora Extracts

Martiningsih

Mudianta

Suryanti

2021

IOP Conf. Ser.: Mater. Sci. Eng.

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This study aims to identify the secondary metabolite compounds and to test the antioxidant activity of ethanol extract, n-hexane fraction, and chloroform fraction of Hippobroma longiflora leaves. Extraction was carried out by the maceration method using ethanol. The resulting crude ethanol extract was then partitioned with n-hexane and chloroform. Each extract and fraction was then tested by phytochemical screening. Antioxidant activity testing was carried out using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. The phytochemical screening test showed that the ethanol extract contained flavonoids, saponins, triterpenoids, and alkaloids; the n-hexane fraction contained steroids, and alkaloids; and the chloroform fraction contained flavonoids, steroids, and alkaloids. Analysis of the antioxidant activity revealed the following Inhibitory Concentration (IC50 values): 9.57 ppm of ethanol extract, 99.59 ppm of n-hexane fraction, 48.54 ppm of chloroform fraction, and 4.30 ppm of ascorbic acid. Based on these results, the antioxidant activity of the ethanol extract was more potential than the n-hexane and chloroform fractions, but smaller than ascorbic acid.

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Gaharu Leaf Extract Water Reduce MDA and 8-OHdG Levels and Increase Activities SOD and Catalase in Wistar Rats Provided Maximum Physical Activity

Cited by 5 publications

References 2 publications

Encapsulation of Kecombrang (Etlingera elatior) Flowers Extract Using the Ionic Gelation Method

Encapsulation of Kecombrang (Etlingera elatior) Flowers Extract Using the Ionic Gelation Method

The oxidative-stress level determine patientâ€™s outcomes with a severe head injury at Sanglah General Hospital, Denpasar, Indonesia

Phytochemical Screening and Antioxidant Activity of Hippobroma longiflora Extracts

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