Fusarium vascular wilt in lentil: Inheritance and identification of DNA markers for resistance

Abstract

The inheritance of resistance to lentil (Lens culinaris Medik.) vascular wilt caused by Fusarium oxysporum f.sp. lentis was investigated in a cross between resistant (ILL5588) and susceptible (L692–16‐l(s)) lines. F2:4 progenies and F6:8, F6:9 recombinant inbred line (RIL) populations were assessed for their wilt reaction for three seasons in a well‐established wilt‐sick plot. Resistance to wilt was conditioned by a single dominant gene in the populations studied. The map location of the Fw locus was identified for the first time through linkage to a random amplified polymorphic DNA (RAPD) marker (OPK‐15900) at 10.8 cM. Two other RAPD markers (OP‐BH800 and OP‐DI5500) identified by bulked segregant analysis were associated in the coupling phase with the resistance trait, and another marker (OP‐C04650) was associated with repulsion. The DNA markers reported here will provide a starting point in marker‐assisted selection for vascular wilt resistance in lentil.

Plant Responses to Elevated Temperatures: A Field Study on Phenological Sensitivity and Fitness Responses to Simulated Climate Warming

“…Frontiers in Plant Science | Plant Genetics and Genomics gene was confirmed (Eujayl et al, 1998b;Hamwieh et al, 2005). However, only SSR59-2B was closely linked with Fw at 19.7 cM (Hamwieh et al, 2005).…”

“…: 31 October 2014;accepted: 30 January 2015;published online: 23 February 2015. Citation: Kumar S, Rajendran K, Kumar J, Hamwieh A andBaum M (2015) …”

“…Earlier studies have used RFLP, AFLP, and RAPD markers to assess genetic diversity and phylogenetic analyses within and among Lens species (Havey and Muehlbauer, 1989;Aboelwafa et al, 1995;Sharma et al, 1995Sharma et al, , 1996Ahmad and McNeil, 1996;Ford et al, 1997) and gene mapping (Eujayl et al, 1998b;Tullu et al, 2003;Duran et al, 2004;Kahraman et al, 2004;Hamwieh et al, 2005). As a part of the CGIAR's Generation Challenge Program (GCP), ICARDA has identified a composite collection of lentil germplasm and characterized them by using SSR markers.…”

Screening of Lentil (Lens culinaris Medikus sub sp. culinaris) Germplasm against Fusarium Wilt (Fusarium oxysporum f. sp. lentis)

“…The method for screening in wilt sick plot has been described by , Bayaa et al, (1995Bayaa et al, ( , 1997, and Eujayl et al, (1998). The experimental material for the present study comprised of 93 lentil genotypes.…”

Evaluation of lentil cultivars and lines for resistance to Fusarium oxysporumf.sp. lentis

“…Sources of resistance to wilt have been found in the cultivated lentil (Bayaa and Erskine, 1990;Mohammadi et al, 2012;Pouralibaba et al, 2016) and lentil wild relatives (Bayaa et al, 1995). Resistance of lentil to Fol is controlled by major genes (Eujayl et al, 1998). For many years, it was believed that no races/pathotypes are existing in the Fol population (Bayaa et al., 1997), but pathogenic and genetic variability of the pathogen has been reported from Algeria, India and Iran (Belabid and Fortas, 2002; Belabid et al, 2004).…”