Screening of Lentil (Lens culinaris Medikus sub sp. culinaris) Germplasm against Fusarium Wilt (Fusarium oxysporum f. sp. lentis)

Meena, Jitendra Kumar; Singh, Akanksha; Dikshit, Harsh Kumar; Mishra, Gyan P.; Aski, Muraleedhar S.; Srinivasa, N.; Gupta, Soma; Singh, Dilip K.; Tripathi, Archana

doi:10.20546/ijcmas.2017.611.298

Cited by 12 publications

(2 citation statements)

References 5 publications

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“…Among biotic stresses fungal diseases are most common which greatly reduces the crop production and productivity. Among fungal diseases, ascochyta blight and fusarium wilt are major diseases which reduces its yield remarkably" [15,16,17]. "Wilt in lentil, caused by Fusarium oxysporum f.sp lentis (Fol), is a one of the major cause of crop loss on every continent except Australia where lentils are produced" [18,19,20].…”

Section: Introductionmentioning

confidence: 99%

Screening of Lentil Genotypes Against Wilt of Lentil (Lens culinaris Medik L.) Caused by Fusarium oxysporum F. SP. Lentis in Glasshouse Condition

Pandey,

Chandra,

Raghuvanshi

et al. 2024

JSRR

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Wilt of lentil is most damaging disease of lentil caused by the fungus Fusarium oxysporum f. sp. lentis. It causes severe crop damage from vegetative to reproducing stage which results in yield reduction. Fol isolates shows high variability and aggressiveness according to the climatic condition. So to overcome from this disease there are many ways of management like by cultural, mechanical, biological and chemical practices. Resistant variety selection is one of the best ecofriendly cultural management of the disease. So for this purpose one hundred genotypes were taken and screened through pot screening technique under glasshouse was carried out at the Student Instructional Farm (SIF), in the department of Plant Pathology, Acharya Narendra Deva University of Agriculture and Technology, Kumarganj, Ayodhya, which is situated in the IndoGangatic plains of eastern Uttar Pradesh at a latitude of 26.470 and an altitude of 113 meters above sea level during Rabi 2020-21. One hundred genotypes of lentil were obtained from the Department of Genetics and Plant breeding, N.D. University of Agriculture and Technology, Kumarganj, Ayodhya and IIPR, Kalyanpur, Kanpur (U.P.).Out of 100 genotypes, 41 genotypes were found highly resistant, 30 genotypes resistant, 8 genotypes moderately resistant, 10 genotypes susceptible and 11 genotypes highly susceptible to Fusarium wilt.

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Section: Introductionmentioning

confidence: 99%

Screening of Lentil Genotypes Against Wilt of Lentil (Lens culinaris Medik L.) Caused by Fusarium oxysporum F. SP. Lentis in Glasshouse Condition

Pandey,

Chandra,

Raghuvanshi

et al. 2024

JSRR

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“…Wild lentil species have been identified as a potential source of resistance that can be tailored for resistance breeding (Singh et al, 2020). Multi-location screening of germplasms identifies germplasms that are resistant to multiple races present in a geographic region (Meena et al, 2017). Conventionally, the resistance (R) gene is isolated by transposon tagging and mapbased cloning, which is laborious and time-consuming.…”

Section: Introductionmentioning

confidence: 99%

Resistance screening and in silico characterization of cloned novel RGA from multi-race resistant lentil germplasm against Fusarium wilt (Fusarium oxysporum f. sp. lentis)

et al. 2023

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Fusarium wilt caused by Fusarium oxysporum f. sp. lentis (Fol) is the most devastating disease of lentil present worldwide. Identification of multi-race fusarium wilt resistance genes and their incorporation into existing cultivars will help to reduce yield losses. In the present study, 100 lentil germplasms belonging to seven lentil species were screened against seven prevalent races of Fol, and accessions IC201561 (Lens culinaris subsp. culinaris), EC714243 (L. c. subsp. odemensis), and EC718238 (L. nigricans) were identified as resistant. The typical R gene codes for the nucleotide-binding site and leucine-rich repeats (NBS-LRR) at the C terminal are linked to either the Toll/interleukin 1-like receptor (TIR) or coiled coil (CC) at the N terminal. In the present study, degenerate primers, designed from the NBS region amplifying the P-loop to the GLPLA motif, isolated forty-five resistance gene analogues (RGAs) from identified resistant accessions. The sequence alignment identified both classes of RGAs, TIR and non-TIR, based on the presence of aspartate (D) and tryptophan (W) at the end of the kinase motif, respectively. The phylogenetic analysis grouped the RGAs into six classes, from LRGA1 to LRGA6, which determined the diversity of the RGAs present in the host. Grouping of the RGAs identified from Lens nigricans, LnRGA 2, 9, 13 with I2 revealed the structural similarity with the fusarium resistance gene. The similarity index ranged from 27.85% to 86.98% among the RGAs and from 26.83% to 49.41% among the known R genes, I2, Gpa2, M, and L6. The active binding sites present along the conserved motifs grouped the RGAs into 13 groups. ADP/ATP, being the potential ligand, determines the ATP binding and ATP hydrolysis activity of the RGAs. The isolated RGAs can be used to develop markers linked to the functional R gene. Furthermore, expression analysis and full-length gene isolation pave the path to identifying the molecular mechanism involved in resistance.

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Lentil Breeding

Dikshit

Mishra

Aski

et al. 2022

Fundamentals of Field Crop Breeding

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Screening of Lentil (Lens culinaris Medikus sub sp. culinaris) Germplasm against Fusarium Wilt (Fusarium oxysporum f. sp. lentis)

Cited by 12 publications

References 5 publications

Screening of Lentil Genotypes Against Wilt of Lentil (Lens culinaris Medik L.) Caused by Fusarium oxysporum F. SP. Lentis in Glasshouse Condition

Screening of Lentil Genotypes Against Wilt of Lentil (Lens culinaris Medik L.) Caused by Fusarium oxysporum F. SP. Lentis in Glasshouse Condition

Resistance screening and in silico characterization of cloned novel RGA from multi-race resistant lentil germplasm against Fusarium wilt (Fusarium oxysporum f. sp. lentis)

Lentil Breeding

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