Fusarium toxins and total fungal biomass indicators in naturally contaminated wheat samples from north-eastern Poland in 2003

Perkowski, J.; Wiwart, Marian; Buśko, Maciej; Laskowska, Magdalena; Berthiller, Franz; Kandler, Wolfgang; Krska, Rudolf

doi:10.1080/02652030701416566

Cited by 33 publications

(25 citation statements)

References 20 publications

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“…In terms of studies determining ATP content in grain, little has been done to date. Still it was stated that the amount of ATP in grain is dependent on the type of analysed cereal Perkowski et al, 2007), which was also confirmed in this study.…”

Section: Mycoflorasupporting

confidence: 75%

Differences in metabolomic profiles of the naturally contaminated grain of barley, oats and rye

Perkowski

Stuper

Buśko

et al. 2012

Journal of Cereal Science

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Section: Mycoflorasupporting

confidence: 75%

Differences in metabolomic profiles of the naturally contaminated grain of barley, oats and rye

Perkowski

Stuper

Buśko

et al. 2012

Journal of Cereal Science

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“…The latter is particularly important in the case of infection by toxigenic fungi such as Fusarium whose products, known as fusariotoxins, are typically very stable and remain in plant tissues, including the grain even in the absence of the longer-lived hyphae of the fungus. In a previous work also by the authors of this paper, it was shown that especially in conditions of massive infection, which takes place during the inoculation of plant with fungus, there is a significant correlation between the concentration of the ERG, and the concentration of trichothecenes (Perkowski et al, 2007). However, in conditions of natural infestation, which highlight the role of other factors, particularly agro-meteorological and the defense mechanisms of plants, the obtained values of correlation coefficients of ERG with trichothecenes often proved to be statistically insignificant.…”

Section: Introductionmentioning

confidence: 66%

“…Essentially it is possible to isolate the ATP from the surface of grains and determine the level of total living biomass. Work conducted previously, particularly in inoculation experiments has revealed a significant correlation of surface ATP levels, with both the concentration of the ERG and trichothecenes (Perkowski et al, 2007). …”

Section: Introductionmentioning

confidence: 91%

“…An extremely important aspect of these studies is connected with analyses of metabolomic profiles of microorganisms, particularly pathogenic, and toxigenic (Panagiotou et al, 2005), with a significant role being played by those metabolites which, being formed in grain, are frequently precursors for the formation of others (Perkowski et al, 2008a). Of these the most frequently analyzed metabolites to date are ergosterol (ERG) (Müller and Schwadorf, 1999; Perkowski et al, 2008b) and to a lesser extent ATP (Suberkropp et al, 1993; Perkowski et al, 2007). …”

Section: Introductionmentioning

confidence: 99%

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Comparison of Volatiles Profile and Contents of Trichothecenes Group B, Ergosterol, and ATP of Bread Wheat, Durum Wheat, and Triticale Grain Naturally Contaminated by Mycobiota

et al. 2016

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In natural conditions cereals can be infested by pathogenic fungi. These can reduce the grain yield and quality by contamination with mycotoxins which are harmful for plants, animals, and humans. To date, performed studies of the compounds profile have allowed for the distinction of individual species of fungi. The aim of this study was to determine the profile of volatile compounds and trichothecenes of group B, ergosterol, adenosine triphosphate content carried out on a representative sample of 16 genotypes of related cereals: triticale, bread wheat, and durum wheat. Based on an analysis of volatile compounds by means of gas chromatography mass spectrometry and with the use of an electronic nose, volatile profiles for cereals were determined. Differentiation is presented at four levels through discriminant analysis, heatmaps, principal component analysis (PCA), and electronic nose maps. The statistical model was built by subsequent incorporation of chemical groups such as trichothecenes (GC/MS), fungal biomass indicators ergosterol (HPLC) and ATP (luminometric) and volatiles. The results of the discriminatory analyses showed that the volatile metabolites most markedly differentiated grain samples, among which were mainly: lilial, trichodiene, p-xylene. Electronic nose analysis made it possible to completely separate all the analyzed cereals based only on 100 ions from the 50–150 m/z range. The research carried out using chemometric analysis indicated significant differences in the volatile metabolites present in the grain of bread wheat, durum wheat and triticale. The end result of the performed analyses was a complete discrimination of the examined cereals based on the metabolites present in their grain.

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“…sporotrichioides, like F. acuminatum, F. sambucinum and F. poae, produce type A trichothecenes: T-2 toxin, HT-2 toxin, T-2 tetraol, T-2 triol, scirpentriol (STO), and diacetoxyscirpenol (DAS). Those mycotoxins are accumulated in infected grain (Brown et al 2001;Mateo et al 2002;Moss 2002;Moss and Thrane 2004;Niesen et al 2004;Perkowski and Basiński 2002;Perkowski et al 2003;Perkowski et al 2007;Thrane et al 2004).…”

Section: Introductionmentioning

confidence: 99%

Fusarium Sporotrichioides Sherb. Toxins Evaluated in Cereal Grain with Trichoderma Harzianum

Wiśniewska¹,

Basiński²,

Chełkowski³

et al. 2011

Journal of Plant Protection Research

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Fusarium head blight is one of the most severe diseases of small grain cereals and is caused by several toxigenic Fusarium species. Yield losses and mycotoxin accumulation in grain are caused by the disease. F. sporotrichioides and F. poae produce type A trichothecenes. Saprophyte fungal antagonists, especially Trichoderma harzianum, are effective biocontrol agents against several fungal soil-borne plant pathogens. These fungal antagonists can reduce the production of Fusarium spp. mycotoxins in some crop plants. The aim of this study was to examine the influence of T. harzianum AN4 on the production of type A trichothecenes by F. sporotrichioides in cereals. The accumulation of six trichothecene mycotoxins (scirpentriol, i.e. STO, T-2 tetraol, T-2 triol, HT-2 toxin i.e., HT-2, T-2 toxin i.e., T-2, diacetoxyscirpenol, i.e., DAS) was reduced on average, by over 89% in bioassays of F. sporotrichioides and Trichoderma isolate AN4 on a liquid medium and on solid substrates (seeds of naked and husked oats and wheat). The reduction in our experiments depended on fungal isolate and substrate. From the three isolates of F. sporotrichioides used in the experiments, the highest accumulation of all the metabolites after 21 days by F. sporotrichioides in nearly all substrates, was recorded for strain ZFR 159. On the liquid medium inoculated with F. sporotrichioides ZFR 159, the amount of type A trichothecenes was the lowest (STO, T-2 tetraol, and T-2 triol not detected, HT-2 toxin 0.02 ppm, DAS 0.10 ppm, T-2 toxin 0.99 ppm). The highest total concentration of these toxins was produced by this isolate in husked oat cv. German (180.16 ppm), but in naked oat cv. Akt the toxin concentration was low (27.62 ppm). Trichothecene accumulation by T. harzianum AN4 was reduced the most in oat cv. Akt (98.48%) in the liquid medium (98.22%), while the lowest reduction was in oat cv. German (48.77%). The non-toxigenic T. harzianum AN4 isolate proved to be a useful biocontrol agent against the toxigenic F. sporotrichioides in cereals, significantly reducing the production of six type A trichothecenes. This is the first report on effective biocontrol of F. sporotrichioides in cereals by T. harzianum.

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Fusarium toxins and total fungal biomass indicators in naturally contaminated wheat samples from north-eastern Poland in 2003

Cited by 33 publications

References 20 publications

Differences in metabolomic profiles of the naturally contaminated grain of barley, oats and rye

Differences in metabolomic profiles of the naturally contaminated grain of barley, oats and rye

Comparison of Volatiles Profile and Contents of Trichothecenes Group B, Ergosterol, and ATP of Bread Wheat, Durum Wheat, and Triticale Grain Naturally Contaminated by Mycobiota

Fusarium Sporotrichioides Sherb. Toxins Evaluated in Cereal Grain with Trichoderma Harzianum

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