Differences in metabolomic profiles of the naturally contaminated grain of barley, oats and rye

Perkowski, J.; Stuper, Kinga; Buśko, Maciej; Góral, Tomasz; Kaczmarek, Anna; Jeleń, Henryk H.

doi:10.1016/j.jcs.2012.07.012

Cited by 23 publications

(22 citation statements)

References 22 publications

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“…Besides its utility to screen for resistance traits, metabolomics is also widely applied as diagnostic tool. For instance, metabolomic analysis of naturally contaminated oat, rye and barley grains yielded distinct patterns of metabolites in infected versus non-infected grains (Perkowski et al, 2012). Moreover, in the same study, mycotoxins could also be analyzed in parallel to the plant metabolites, demonstrating the power of metabolomics as diagnostic aid.…”

Section: Discussionmentioning

confidence: 99%

Metabolomics of cereals under biotic stress: current knowledge and techniques

Balmer¹,

Flors²,

Glauser³

et al. 2013

Front. Plant Sci.

126

108

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Prone to attacks by pathogens and pests, plants employ intricate chemical defense mechanisms consisting of metabolic adaptations. However, many plant attackers are manipulating the host metabolism to counteract defense responses and to induce favorable nutritional conditions. Advances in analytical chemistry have allowed the generation of extensive metabolic profiles during plant-pathogen and pest interactions. Thereby, metabolic processes were found to be highly specific for given tissues, species, and plant-pathogen/pest interactions. The clusters of identified compounds not only serve as base in the quest of novel defense compounds, but also as markers for the characterization of the plants' defensive state. The latter is especially useful in agronomic applications where meaningful markers are essential for crop protection. Cereals such as maize make use of their metabolic arsenal during both local and systemic defense responses, and the chemical response is highly adapted to specific attackers. Here, we summarize highlights and recent findings of metabolic patterns of cereals under pathogen and pest attack.

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Section: Discussionmentioning

confidence: 99%

Metabolomics of cereals under biotic stress: current knowledge and techniques

Balmer¹,

Flors²,

Glauser³

et al. 2013

Front. Plant Sci.

126

108

View full text Add to dashboard Cite

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“…(Perkowski et al, 2012;Gu et al, 2015;Sánchez-Martín et al, 2015). Во всех случаях прослеживается как практический, так и теоретический аспект этого вопроса.…”

Section: генетические ресурсы растенийunclassified

The metabolomic approach to the comparative analysis of wild and cultivated species of oats (Avena L.)

Лоскутов¹,

Шеленга²,

Конарев³

et al. 2016

Vestn. VOGiS

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Seed metabolomic profiles have been investigated in wild and cultivated forms (cultivars) of oat (Avena L.). Seed accessions from the VIR oat collection were used for the research. Metabolomic analysis employed gas liquid chromatography-mass spectrometry (GLC-MS) using an Agilent 6850 chromatographer (USA). The analysis covered the composition and content of organic and fatty acids, amino acids, polyatomic spirits and sugars. The content fluctuation range for the studied groups of compounds was found to be narrower (significantly in some cases) in cultivars than in the wild species. Along with a sharp increase in oleic acid content, cultivars demonstrated a decrease in that of linoleic acid. The general conclusions from the comparison of seed metabolomic profiles in wild species and cultivars are presented below. A number of wild species can be recommended as a potential source of biochemical quality traits for breeding purposes. A series of metabolites (compounds), the content of which changes during domestication or which differentiate wild oat species from cultivars has been identified was found. Along with such well-known healthy food chemical factors as oleic acid, glucose and fructose, etc., differences concerning monoacylglycerol compounds (MAG 16 : 0 and MAG-2 18 : 2, etc.) have been found. The latter have been proposed to be related to the formation of adaptive traits, in particular, resistance to diseases and pests, and to environmental abiotic stresses.Key words: Avena L.; wild species; varieties, seeds; gas chromatography; mass spectrometry; metabolomics; amelioration; adaptability and polymorphism of characters.Исследовались метаболомные спектры зерновок диких и куль ти-вируемых форм (сортов) овса (Avena L.). Материалом для изучения служили образцы семян из коллекции ВИР. Метаболомные иссле-дования (анализ метаболома) проводили методом газожидкостной хроматографии с масс-спектрометрией (ГЖХ-МС) на хроматографе Agilent 6850 (CША). Анализировали состав и содержание органи-ческих и жирных кислот, аминокислот, многоатомных спиртов и сахаров. Установлено, что у сортов по сравнению с дикими видами сузился (в ряде случаев существенно) диапазон колеба-ния значений содержания изученных групп соединений. Наряду с резким увеличением содержания олеиновой кислоты у сортов снизилось содержание линолевой кислоты. В целом сравнение метаболомных спектров зерновок диких видов и сортов позво-лило сделать следующие основные выводы. Ряд дикорастущих видов овса может быть рекомендован в качестве потенциального источника биохимических признаков качества при селекции. Выявлены метаболиты, содержание которых меняется в процессе окультуривания или по которым дикие виды овса отличаются от сортов этой культуры. Среди этих соединений наряду с такими широко известными составляющими здорового питания, как олеиновая кислота, глюкоза, фруктоза и др., выявлены моноацил-глицеролы -МАГ 16 : 0, МАГ-2 18 : 2 и др. Высказано предположение, что последние могут быть связаны с формированием признаков адаптивности, в частности с устойчивость...

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“…(). VOCs produced by fungi include alcohols, ketones, aldehydes, hydrocarbons, monoterpenes, sesquiterpenes, furans, esters, and so on (Lippolis, Pascale, Cervellieri, Damascelli, & Visconti, ; Olsson, Borjesson, Lundstedt, & Schnurer, ; Perkowski et al., ).…”

Section: Introductionmentioning

confidence: 99%

Identification of Volatile Organic Compounds and Their Concentrations Using a Novel Method Analysis of MOS Sensors Signal

Gancarz

Nawrocka

Rusinek

2019

Journal of Food Science

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Volatile organic compounds (VOCs) are natural markers useful in rapid assessment of adverse changes occurring in biological material. The use of an electronic nose seems to be a good, fast, and cheap method to determine particular VOCs. This paper presents a new method determination for VOCs and their concentration based on three sensorgram parameters: maximum of normalized sensor response, reaction time, and cleaning time measured from the end of the test to the half value of the maximum of normalized sensor response. The novelty of the method consists in the use for the first time of two parameters: reaction time and cleaning time measured from the end of the test to the half value of the maximum of normalized sensor response. The VOC sensorgrams at different VOC concentrations (26 to 3,842 ppm) were measured by an electronic nose Food Volatile Compound Analyzer (Agrinose) equipped with eight metal oxide semiconductor sensors dedicated to detect different gases. In the present studies, only six sensors that best respond to the VOCs were used. The highest responses to VOCs were obtained for two sensors—TGS2602 and AS‐MLV‐P2. The results showed that the dependence between the sensorgram parameters on VOC concentration was well described by a logarithmic curve in the whole range of concentrations. Detailed analysis revealed that the cleaning time increases with an increase in the number of carbon atoms in aliphatic molecules. The principal component analysis (PCA) was used to verify the utility of the new three parameters method in VOCs differentiation. The PCA analysis of these parameters showed that maximum of the normalized sensor response alone, which has been used for identification of particular VOCs so far, could not be regarded as a good parameter used for this purpose. Application of all the three parameters gave the best results in VOC identification. The research indicates that the use of three parameters of a volatile compound instead of only one parameter can allow precise determination of substances. Moreover, the results indicate that the analyzed parameters depend on the chemical structure of VOCs.

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Differences in metabolomic profiles of the naturally contaminated grain of barley, oats and rye

Cited by 23 publications

References 22 publications

Metabolomics of cereals under biotic stress: current knowledge and techniques

Metabolomics of cereals under biotic stress: current knowledge and techniques

The metabolomic approach to the comparative analysis of wild and cultivated species of oats (Avena L.)

Identification of Volatile Organic Compounds and Their Concentrations Using a Novel Method Analysis of MOS Sensors Signal

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