Fusarium oxysporum f. sp. albedinis Toxin Characterization and Use for Selection of Resistant Date Palm to Bayoud Disease

Sedra, M.H.; Lazrek, B. H.

doi:10.1007/978-94-007-1318-5_13

Cited by 22 publications

(16 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The result culture fi ltrate was stored in the refrigerator until utilization. The extraction of fraction FII was done according to the method described in pervious works (Sedra and Lazrek, 2011) (Fig. 1).…”

Section: Preparation Of Culture Fi Ltrate and Toxin Extractionmentioning

confidence: 99%

“…Germinated seeds were surface sterilized as above and put in tubes containing 20 ml of sterilized agar medium with the addition of the toxin fraction (FII or CF) at different concentrations (25, 50 and 75 μg/ml) and disinfected trough fi ltration at 0.45 μm according to the method developed by Sedra and Lazrek (2011). A total of 10 replications per toxic element were used and repeated 3 times.…”

Section: Seedling Bioassaymentioning

confidence: 99%

“…In this context, Foa is able to produce toxic metabolites in a synthetic medium, which can be biochemically purifi ed and characterized from the culture fi ltrate of aggressive strains (El Fakhouri et al, 1996a;Sedra et al, 1998). Rapid tests using Foa toxins on detached leafl ets, complete young plantlets and seedlings of date palm have been developed (Sedra et al, 1993;Sedra and Lazrek, 2011). Previous studies of Foa toxin extract showed that they produce similar symptoms (rolling and withering of leaves) in detached date palm leaves bioassays compared to pathogenicity tests conducted by pathogen inoculation of date palm plants (Sedra et al, 1998), also pathogenic and saprophytic strains of Fusarium oxysporum produce different biochemical profi les of toxins (Amraoui et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

A relationship between Bayoud disease severity and toxin susceptibility of date palm cultivars

Oubraim¹,

Sedra

Lazrek³

2016

Emir. J. Food Agric

View full text Add to dashboard Cite

The Bayoud disease, caused by Fusarium oxysporum f. sp. albedinis (Foa, constitutes a serious threat to the date palm plantations in Morocco, Algeria and Mauritania. Three isolates of Foa were in vivo and in vitro examined for their pathogenicity and effect of culture fi ltrate phytotoxicicity. Our results indicated that, the agressivity of Foa strains was related to the effect observed with their culture fi ltrate and toxic fraction FII, which contains fusaric acid as a major compound, on detached leaves and seedling of date palm susceptible cultivars. This relationship between pathogenity and phytotoxic effect raises the question of the role of those phytotoxic compounds on beyond pathogenicity, and consequently their key role on breeding for resistance.

show abstract

Section: Preparation Of Culture Fi Ltrate and Toxin Extractionmentioning

confidence: 99%

Section: Seedling Bioassaymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

A relationship between Bayoud disease severity and toxin susceptibility of date palm cultivars

Oubraim¹,

Sedra

Lazrek³

2016

Emir. J. Food Agric

View full text Add to dashboard Cite

show abstract

“…In order to control the bayoud disease in date palm, caused by the fungus Fusarium oxysporum f. sp. albedinis (Phoenix dactylifera L.), Sedra and Lazrek [24] have presented a methodology based on the selection of resistant cultivars of date palm. The use of pathogen toxins in vitro selection was the approach used by these authors for rapid screening for date palm resistance to bayoud disease.…”

Section: Discussionmentioning

confidence: 99%

New Insights for Diagnosis of Pineapple Fusariosis by MALDI-TOF MS Technique

2016

View full text Add to dashboard Cite

Fusarium is one of the most economically important fungal genus, since it includes many pathogenic species which cause a wide range of plant diseases. Morphological or molecular biology identification of Fusarium species is a limiting step in the fast diagnosis and treatment of plant disease caused by these fungi. Mass spectrometry by matrix-assisted laser/desorption ionisation-time-of-flight (MALDI-TOF)-based fingerprinting approach was applied to the fungal growth monitoring and direct detection of strain Fusarium guttiforme E-480 inoculated in both pineapple cultivars Pérola and Imperial side shoots, that are susceptible and resistant, respectively, to this fungal strain. MALDI-TOF MS technique was capable to detect fungal molecular mass peaks in the susceptible pineapple stem side shoot tissue. It is assumed that these molecular masses are mainly constituted by ribosomal proteins. MALDI-TOF-based fingerprinting approach has herein been demonstrated to be sensitive and accurate for the direct detection of F. guttiforme E-480 molecular masses on both susceptible and resistant pineapple side stem free of any pre-treatment. According to the results obtained, the changing on molecular mass peaks of infected susceptible pineapple tissue together with the possibility of fungal molecular masses analysis into this pineapple tissue can be a good indication for an early diagnosis by MALDI-TOF MS of pineapple fusariosis.

show abstract

“…albedinis) (Quenzar et al, 2001) is by far the most dangerous disease affecting date palm plantations (Sedra and Lazrak, 2011). In Morocco, the majority of the date palm groves are affected by this fungus (Quenzar et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

Effect of Basal Medium, Explants Size and Density on the In Vitro Proliferation and Growth of Date Palm (Phoenix dactylifera L.) Cultivar ‘16-bis’

Mazri¹

2013

Not Sci Biol

View full text Add to dashboard Cite

The effect of basal medium, explant size and density on shoot multiplication, growth, rooting and acclimatization of date palm cv. '16-bis' was evaluated. Bud clusters of different sizes (2, 3, 4 and 5 buds per cluster) were cultured at density of 1, 2, 3 and 4 clusters on Murashige and Skoog medium (MS), woody plant medium (WPM) and Nitsch medium (NM) supplemented with 0.5 mg/L 2-naphthoxyacetic acid and 0.5 mg/L kinetin for three months (multiplication phase). Separated shoots of different sizes (<3 cm; 3 to 4.5 cm and 4.5 to 6 cm) were cultured at density of 1, 2, 3 and 4 shoots on hormone free MS medium, WPM or NM for three months (Elongation-rooting phase). The proliferation and development of shoots were affected by the basal medium, explant size and density. The optimal shoot proliferation (18.1) was observed when 4 buds clusters were cultured at the density of 2 clusters per jar in MS medium. Separated shoots of 4.5 to 6 cm length exhibited the optimal in vitro development in terms of leaf length and greening, and root number and length when cultured on MS medium. In addition, these shoots reached the highest acclimation frequency with 80%. Our results would be utilized for an efficient propagation of plantlets of cv. '16-bis' , a selected date palm cultivar resistant to the bayoud disease.

show abstract

Fusarium oxysporum f. sp. albedinis Toxin Characterization and Use for Selection of Resistant Date Palm to Bayoud Disease

Cited by 22 publications

References 49 publications

A relationship between Bayoud disease severity and toxin susceptibility of date palm cultivars

A relationship between Bayoud disease severity and toxin susceptibility of date palm cultivars

New Insights for Diagnosis of Pineapple Fusariosis by MALDI-TOF MS Technique

Effect of Basal Medium, Explants Size and Density on the In Vitro Proliferation and Growth of Date Palm (Phoenix dactylifera L.) Cultivar ‘16-bis’

Contact Info

Product

Resources

About