New Insights for Diagnosis of Pineapple Fusariosis by MALDI-TOF MS Technique

Santos, Cledir; Ventura, José Aires; Lima, Nelson

doi:10.1007/s00284-016-1041-9

Cited by 12 publications

(7 citation statements)

References 24 publications

(35 reference statements)

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“…Additionally, mass-spectrometry-based approaches have also been applied successfully for the identification and differentiation of Fusarium species based on spore protein profiles [ 37 , 38 ]. Furthermore, MALDI-TOF MS peptide/protein fingerprints have been used to distinguish closely related species in the Fusarium fujikuroi species complex [ 39 ], and for in situ diagnosis in infected plant tissue [ 40 ]. Here, we have tested the potential of MALDI-TOF MS to distinguish the used Fusarium species based on protein fingerprints of mycelium.…”

Section: Discussionmentioning

confidence: 99%

Species-Specific Impact of Fusarium Infection on the Root and Shoot Characteristics of Asparagus

et al. 2020

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Soil-borne pathogens can have considerable detrimental effects on asparagus (Asparagus officinalis) growth and production, notably caused by the Fusarium species F. oxysporum f.sp. asparagi, F. proliferatum and F. redolens. In this study, their species-specific impact regarding disease severity and root morphological traits was analysed. Additionally, various isolates were characterised based on in vitro physiological activities and on protein extracts using matrix-assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI-TOF MS). The response of two asparagus cultivars to the different Fusarium species was evaluated by inoculating experiments. Differences in aggressiveness were observed between Fusarium species and their isolates on roots, while no clear disease symptoms became visible in ferns eight weeks after inoculation. F. redolens isolates Fred1 and Fred2 were the most aggressive strains followed by the moderate aggressive F. proliferatum and the less and almost non-aggressive F. oxysporum isolates, based on the severity of disease symptoms. Fungal DNA in stem bases and a significant induction of pathogenesis-related gene expression was detectable in both asparagus cultivars. A significant negative impact of the pathogens on the root characteristics total root length, volume, and surface area was detected for each isolate tested, with Fred1 causing the strongest effects. No significant differences between the tested asparagus cultivars were observed.

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Section: Discussionmentioning

confidence: 99%

Species-Specific Impact of Fusarium Infection on the Root and Shoot Characteristics of Asparagus

et al. 2020

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“…Fusariosis caused by Fusarium guttiforme (formerly Fusarium moniliforme var. subglutinans, Fusarium subglutinans) appeared in Brazil in the 1960s and became the major concern for producers in that region [9][10][11][12][13]. The name "Fusarium guttiforme" brought about confusion, and some articles dealing with FCR used it to describe the pathogen [14,15].…”

Section: Introductionmentioning

confidence: 99%

Diversity and Toxigenicity of Fungi that Cause Pineapple Fruitlet Core Rot

Barral

Chillet

Doizy

et al. 2020

Toxins

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The identity of the fungi responsible for fruitlet core rot (FCR) disease in pineapple has been the subject of investigation for some time. This study describes the diversity and toxigenic potential of fungal species causing FCR in La Reunion, an island in the Indian Ocean. One-hundred-and-fifty fungal isolates were obtained from infected and healthy fruitlets on Reunion Island and exclusively correspond to two genera of fungi: Fusarium and Talaromyces. The genus Fusarium made up 79% of the isolates, including 108 F. ananatum, 10 F. oxysporum, and one F. proliferatum. The genus Talaromyces accounted for 21% of the isolated fungi, which were all Talaromyces stollii. As the isolated fungal strains are potentially mycotoxigenic, identification and quantification of mycotoxins were carried out on naturally or artificially infected diseased fruits and under in vitro cultures of potential toxigenic isolates. Fumonisins B1 and B2 (FB1-FB2) and beauvericin (BEA) were found in infected fruitlets of pineapple and in the culture media of Fusarium species. Regarding the induction of mycotoxin in vitro, F. proliferatum produced 182 mg kg⁻1 of FB1 and F. oxysporum produced 192 mg kg⁻1 of BEA. These results provide a better understanding of the causal agents of FCR and their potential risk to pineapple consumers.

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“…The polyphasic approach combines the use of different techniques, such as morphology, biochemical and molecular biology tools and more recently, mass spectrometry (particularly matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF MS). Furthermore, mass spectrometry based microbial spectral analysis has been used as a key step in the polyphasic identification of filamentous fungi from different fields [12,14,22,23,24,25,26,27,28].…”

Section: Introductionmentioning

confidence: 99%

Polyphasic, Including MALDI-TOF MS, Evaluation of Freeze-Drying Long-Term Preservation on Aspergillus (Section Nigri) Strains

et al. 2019

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This study aims to evaluate the effect of freeze-drying and long-term storage on the biotechnological potential of Aspergillus section Nigri strains. Twelve selected strains were freeze-dried and aged by accelerated storage, at 37 °C in the dark, for 2 and 4 weeks. To assess possible changes as a consequence of the ageing in the freeze-drying ampoules, morphological characteristics, mycotoxins and enzymes production, matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALTI-TOF MS) spectra, and M13 phage probe fingerprinting were used as part of a polyphasic approach. Phenotypical changes were observed; nevertheless, they did not substantially affect the potential biotechnological use of these strains. The activity of hydrolytic enzymes (protease, carboxymethylcellulase, xylanase, pectinase and mannanase) was maintained or increased after freeze-drying. MALDI-TOF MS data originated spectra that grouped, for the majority of samples, according to strain independently of preservation time point. M13 profiles revealed the presence of some genetic polymorphisms after preservation. However, the three studied times still clustered for more than 50% of strains. Our results show that the studied strains maintain their biotechnological potential after preservation, with minimal phenotypic alterations. These findings provide evidence that freeze-drying preservation is a suitable option to preserve biotechnologically relevant aspergilli strains from section Nigri, and one should consider that the observed effects might be species/strain-dependent.

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New Insights for Diagnosis of Pineapple Fusariosis by MALDI-TOF MS Technique

Cited by 12 publications

References 24 publications

Species-Specific Impact of Fusarium Infection on the Root and Shoot Characteristics of Asparagus

Species-Specific Impact of Fusarium Infection on the Root and Shoot Characteristics of Asparagus

Diversity and Toxigenicity of Fungi that Cause Pineapple Fruitlet Core Rot

Polyphasic, Including MALDI-TOF MS, Evaluation of Freeze-Drying Long-Term Preservation on Aspergillus (Section Nigri) Strains

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