1983
DOI: 10.1099/00221287-129-8-2677
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Further Evidence for the Existence of a Membrane Potential in Trypanosoma brucei brucei

Abstract: The distribution of 137Cs+, in the presence of valinomycin, has been used to measure the magnitude of the membrane potential (A$) in bloodstream forms of Trypanosoma brucei brucei.Values of the A$ falling in the range -100 mV to -160 rnV were observed and the maintenance of this A$ was sensitive to certain ionophores and protonophores.

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Cited by 3 publications
(2 citation statements)
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“…It would be expected that the distribution of Cst and Rb' would report the K t diffusion potential in the presence of valinomycin equally well. However, it is significant that, in this present study, the extracellular medium contained K ' (6.2 mM), whereas the experiments described by Midgley (1983b) were performed in a K'-free medium, and hence the AY, in the absence of extracellular K + was likely to be hyperpolarized. It should be noted that the distribution of Rb' or Cs' cannot be used to assess an intracellular A Y , since intracellular organelles such as mitochondria usually possesses no passive electrical permeability to K + or its analogues (Scott and Nicholls, 1980).…”
Section: Discussionmentioning
confidence: 83%
“…It would be expected that the distribution of Cst and Rb' would report the K t diffusion potential in the presence of valinomycin equally well. However, it is significant that, in this present study, the extracellular medium contained K ' (6.2 mM), whereas the experiments described by Midgley (1983b) were performed in a K'-free medium, and hence the AY, in the absence of extracellular K + was likely to be hyperpolarized. It should be noted that the distribution of Rb' or Cs' cannot be used to assess an intracellular A Y , since intracellular organelles such as mitochondria usually possesses no passive electrical permeability to K + or its analogues (Scott and Nicholls, 1980).…”
Section: Discussionmentioning
confidence: 83%
“…However, it is only recently that several studies have contributed to a better understanding of the bioenergetics of this protozoon. Early studies [1,2] established the existence of a membrane potential. Recently, the transmembrane potentials (∆Ψ) and the pH gradient (∆pH), components of the proton electrochemical potential (∆µ H + ), were intensively investigated in various laboratories for the bloodstream form of T. brucei brucei using distribution of radioactive cations (Rb + , methyltriphenylphosphonium, tetraphenylphosphonium), weak acids and weak bases.…”
Section: Introductionmentioning
confidence: 99%