1991
DOI: 10.1111/j.1432-1033.1991.tb16390.x
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The distribution of permeant ions demonstrates the presence of at least two distinct electrical gradients in bloodstream forms of Trypanosoma brucei

Abstract: The distribution of 86Rb+ and the radiolabelled lipophilic cation [3H]methyltriphenylphosphonium (MePh3P+) was used to investigate the membrane potentials that exist in bloodstream forms of Trypanosoma brucei. Even after correction for binding to cellular constituents, the accumulation of MePh3P+ was approximately tenfold greater than the accumulation of Rb+ under resting conditions. The addition of low concentrations of carbonylcyanide p‐trifluoromethoxyphenylhydrazone or valinomycin reduced the accumulation … Show more

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Cited by 25 publications
(52 citation statements)
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References 58 publications
(21 reference statements)
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“…and either ['HH]MePh3P+ (10 nM, 30 pCi/ nmol) or [14C]DM0 (20 pM, 54 Ci/mol). After 10 min, the suspension was centrifuged at 8000 g for 20 s through a layer of silicon oil and the accumulation of [3H]MePh3P+ or ['4C]DM0 was measured in an analogous fashion to that described previously for intact trypanosomes (Nolan and Voorheis, 1991). The intramitochondrial and cxtramitochondrial volumes of pellets of isolated mitochondrial vesicles were measured using 3 H 2 0 (0.2 pCi/ml) and [U-'4C]sucrose (0.45 pM, 555 Ci/M).…”
Section: Radiolabelled-ion Uptakementioning
confidence: 99%
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“…and either ['HH]MePh3P+ (10 nM, 30 pCi/ nmol) or [14C]DM0 (20 pM, 54 Ci/mol). After 10 min, the suspension was centrifuged at 8000 g for 20 s through a layer of silicon oil and the accumulation of [3H]MePh3P+ or ['4C]DM0 was measured in an analogous fashion to that described previously for intact trypanosomes (Nolan and Voorheis, 1991). The intramitochondrial and cxtramitochondrial volumes of pellets of isolated mitochondrial vesicles were measured using 3 H 2 0 (0.2 pCi/ml) and [U-'4C]sucrose (0.45 pM, 555 Ci/M).…”
Section: Radiolabelled-ion Uptakementioning
confidence: 99%
“…In every case parallel experiments were performed to measure the intracellular and extracellular spaces in the pellets of cells using 3 H 2 0 and [U-'4C]sucrose as describcd previously (Nolan and Voorheis, 1991). Radiolabelled-ion uptake by mitochondrial vesicles was measured following resuspension of the mitochondrial fraction, isolated from bloodstream forms of T. hrucei (1 mg protein/ ml), in a medium containing 20 mM Mops, pH 7.0, 300 mM sucrose, 2 mM MgC12, fatty-acid-free bovine serum albumin (0.2%, mass/vol.)…”
Section: Radiolabelled-ion Uptakementioning
confidence: 99%
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