1996
DOI: 10.1042/bj3140595
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Kinetic study of the plasma-membrane potential in procyclic and bloodstream forms of Trypanosoma brucei brucei using the fluorescent probe bisoxonol

Abstract: The characteristics of the plasma-membrane potential of procyclic and bloodstream forms of Trypanosoma brucei brucei (cultured cells) were investigated using the fluorescent anionic probe bisoxonol. Observation of a stable and representative plasma-membrane potential in the resting state required careful washing, centrifugation and maintenance of the cells at room temperature before measurement. Bloodstream forms were more prone to depolarization during washing at 4 degrees C than procyclic cells. The higher f… Show more

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Cited by 19 publications
(15 citation statements)
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References 33 publications
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“…1, pink curves). The effect of exogenous K ϩ on ⌿ p in T. brucei has been controversial (11,37). Our results indicate that in T. brucei, bloodstream as well as procyclic forms, the plasma membrane is not immediately penetrable for K ϩ .…”
Section: Phenotypic Characterization Of Kmentioning
confidence: 60%
“…1, pink curves). The effect of exogenous K ϩ on ⌿ p in T. brucei has been controversial (11,37). Our results indicate that in T. brucei, bloodstream as well as procyclic forms, the plasma membrane is not immediately penetrable for K ϩ .…”
Section: Phenotypic Characterization Of Kmentioning
confidence: 60%
“…A multifunctional intracellular ATPase in trypanosomes, TbVCP, is sensitive to NEM inhibition (40). In bloodstream form T. brucei brucei, NEM caused depolarization of the plasma membrane potential, presumably through inhibition of H ϩ -ATPases (41). In addition to ATPases, phosphatases often contain redox-active cysteine residues that are sensitive to oxidation (42).…”
Section: Discussionmentioning
confidence: 99%
“…These two properties make recording the membrane voltage with microelectrodes difficult. As an alternative strategy we monitored the membrane voltage of the host cells with the voltage‐sensitive fluorescent dye bisoxonol [14,15]. A cell suspension was incubated in a solution of 1 μM bisoxonol.…”
Section: Pbcv‐1 Infection May Induce Membrane Depolarization In Host mentioning
confidence: 99%