Fungicidal Effect of Prenylated Flavonol, Papyriflavonol A, Isolated from Broussonetia papyrifera (L.) Vent. Against Candida albicans

Abstract

Papyriflavonol A (PapA), a prenylated flavonoid [5,7,3',4'tetrahydroxy-6,5'-di-(γ,γ-dimethylallyl)-flavonol], was isolated from the root barks of Broussonetia papyrifera. Our previous study showed that PapA has a broad-spectrum antimicrobial activity against pathogenic bacteria and fungi. In this study, the mode of action of PapA against Candida albicans was investigated to evaluate PapA as an antifungal agent. The minimal inhibitory concentration (MIC) values were 10~25 µg/ml for C. albicans and Saccharomyces cerevisiae, Gram-negative bacteria (Escherichia coli and Salmonella typhimurium), and Gram-positive bacteria (Staphylococcus epidermidis and Staphylococcus aureus). The kinetics of cell growth inhibition, scanning electron microscopy, and measurement of plasma membrane florescence anisotrophy revealed that the antifungal activity of PapA against C. albicans and S. cerevisiae is mediated by its ability to disrupt the cell membrane integrity. Compared with amphotericin B, a cell-membranedisrupting polyene antibiotic, the hemolytic toxicity of PapA was negligible. At 10~25 µg/ml of MIC levels for the tested strains, the hemolysis ratio of human erythrocytes was less than 5%. Our results suggest that PapA could be a therapeutic fungicidal agent having potential as a broad spectrum antimicrobial agent.

Effect of Broussonetia papyrifera L. silage on blood biochemical parameters, growth performance, meat amino acids and fatty acids compositions in beef cattle

“…There are considerable studies focusing on reducing the biohydrogenation by adding more unsaturated fatty acids of feedstuff [32,33], protecting unsaturated fatty acids [34] or supplying feedstuff against biohydrogenation [35] in ruminants' diets. In the present study, PUFA were significantly higher in 10% and 15% groups than that in control group or 5% group (p<0.05), and it might due to the antimicrobial activities in the diet supplemented with B. papyrifera silage, as it is reported that prenylated flavonoids isolated from B. papyrifera have antimicrobial activity [36,37] which might affect the rumen microorganism by reducing the biohydrogenation of fatty acids in the rumen, and also dietary antioxidant activity might increase PUFA content in meat [38,39]. We found the similar result in milk of dairy cattle [5].…”

Therapeutic Effect ofBroussonetia papyriferaandLonicera japonicain Ovalbumin-induced Murine Asthma Model

“…Therapeutic efficacy of Broussonetia papyrifera and Lonicera japonica Natural Product Communications Vol. 8 (11) 2013 1613 Statistical analysis: Data are expressed as mean values ± standard error. Graphs were drawn using GraphPad Prism V4.03 (GraphPad Software, San Diego, CA, USA) and statistical significances were analyzed using Microcal Origin student's t-test two populations (Microcal Software, Northampton, MA, USA).…”

“…Papyriflavonol A (PapA), isolated from the root bark of B. papyrifera (L.) Vent. shows activity against pathogenic bacteria and fungi [11]. It also prevents passive cutaneous anaphylaxis [12].…”

The effect of the flavonol morin on adhesion and aggregation of Streptococcus pyogenes

“…More than 4000 flavonoid compounds have been identified as being anti-inflammatory and antioxidative (Beecher, 2003;Lamuela-Ravento et al, 2009). Flavonols are abundant in fruits, vegetables, tea and red wine (Ross & Kasum, 2002;Beecher, 2003); they can inhibit the growth of bacteria and fungi such as Pseudomonas aeruginosa, meticillin-resistant Staphylococcus aureus (MRSA) and Candida albicans, as well as affecting sortase activity (Shure et al, 2006;Lamuela-Ravento et al, 2009;Liu et al, 2009;Escaich, 2010;Jayaraman et al, 2010;John et al, 2010). Morin (3, 5, 7, 2′, 4′-pentahydroxyflavone) is a plant-derived flavonol, which is known to be an effective inhibitor of Gram-positive bacteria (Kang et al, 2006).…”