The effect of the flavonol morin on adhesion and aggregation of Streptococcus pyogenes

Green, Angharad E; Rowlands, Richard S.; Cooper, Rose; Maddocks, Sarah

doi:10.1111/j.1574-6968.2012.02598.x

Cited by 19 publications

(11 citation statements)

References 41 publications

(62 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The variation in the antibiofilm activity among various serotypes might be attributed to the varying amount of EPS layer present in each strain. Similarly to other reported biofilm inhibitors such as the flavanol morin (Green et al 2012) and manuka honey , usnic acid also showed a good reduction in biofilm biomass (Table 1). Bacterial aggregation plays a very important role in adherence to host receptors as well as other bacteria during biofilm development ).…”

Section: Discussionsupporting

confidence: 50%

“…The disturbing observation that one in three tonsillectomies can be colonized with GAS biofilms (Roberts et al 2012) in itself showcases the deleterious affects of GAS biofilms. Like other biofilms of pathogens, GAS biofilms also have been clinically related to antibiotic treatment failure (Baldassarri et al 2006) which has prompted several researchers to explore various natural resources such as medicinal plants (Limsuwan et al 2008;Samoilova et al 2014), plant derived flavanols (Green et al 2012), manuka honey , mangrove plants (Musthafa et al 2013), marine bacteria (Thenmozhi et al 2009;Nithyanand et al 2010) and phage encoded endolysins (Shen et al 2013) as antibiofilm agents against GAS biofilms.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Usnic acid, a lichen secondary metabolite inhibits Group A Streptococcus biofilms

Nithyanand

Shafreen

Muthamil

et al. 2014

Antonie van Leeuwenhoek

View full text Add to dashboard Cite

Group A Streptococci (GAS) are involved in a number of life threatening diseases and biofilm formation by these pathogens are considered as an important virulence determinant as it mediates antibiotic resistance among them. In the present study, we have explored the ability of (+)-usnic acid, a lichen secondary metabolite, as an antibiofilm agent against four serotypes of Streptococcus pyogenes causing pharyngitis. Usnic acid inhibited the biofilms of M serotypes M56, st38, M89 efficiently and the biofilm of M74 to a lesser extent. Confocal imaging of the treated samples showed that usnic acid reduced the biomass of the biofilms when compared to that of the control. Fourier Transfer Infrared (FT-IR) spectroscopy indicated that usnic acid reduced the cellular components (proteins and fatty acids) of the biofilms. Interestingly, the FT-IR spectrum further revealed that usnic acid probably acted upon the fatty acids of the biofilms as evident from the disappearance of a peak at 2,455-2,100 cm(-1) when compared to the control only in serotypes M56, st38 and M89 but not in M74. The present study shows, for the first time, that usnic acid can act as an effective antibiofilm agent against GAS.

show abstract

Section: Discussionsupporting

confidence: 50%

Section: Introductionmentioning

confidence: 99%

Usnic acid, a lichen secondary metabolite inhibits Group A Streptococcus biofilms

Nithyanand

Shafreen

Muthamil

et al. 2014

Antonie van Leeuwenhoek

View full text Add to dashboard Cite

show abstract

“…Morin hydrate (morin), a yellow natural bioflavonoid compound found in many members of the Moraceae family and various fruits and vegetables, has been shown to possess multiple pharmacological effects, including anti-inflammatory, anti-proliferative and anti-oxidant activities (Krol et al, 2002; Fang et al, 2005; Green et al, 2012; Lee et al, 2016). Additionally, several recent studies have shown that morin can significantly attenuate the virulence of pathogenic bacteria (Huang et al, 2014; Wang et al, 2015; Sivaranjani et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Morin Attenuates Streptococcus suis Pathogenicity in Mice by Neutralizing Suilysin Activity

et al. 2017

Front. Microbiol.

View full text Add to dashboard Cite

Streptococcus suis, a Gram-positive pathogen, is widely recognized as an important agent of swine infection, and it is also known to cause a variety of zoonoses, such as meningitis, polyarthritis and pneumonia. Suilysin (SLY), an extracellular pore-forming toxin that belongs to the cholesterol-dependent cytolysin family, is an essential virulence factor of S. suis capsular type 2 (SS2). Here, we found that morin hydrate (morin), a natural flavonoid that lacks anti-SS2 activity, inhibits the hemolytic activity of SLY, protects J774 cells from SS2-induced injury and protects mice from SS2 infection. Further, by molecular modeling and mutational analysis, we found that morin binds to the “stem” domain 2 in SLY and hinders its transformation from the monomer form to the oligomer form, which causes the loss of SLY activity. Our study demonstrates that morin hinders the cell lysis activity of SLY through a novel mechanism of interrupting the heptamer formation. These findings may lead to the development of promising therapeutic candidates for the treatment of SS2 infections.

show abstract

“…Among these, polyphenols, which are classified into flavonoids (e.g., catechin, flavonol, and tannin) and nonflavonoids (e.g., phenolic acid, neolignan), have been especially well studied [11, 12, 13, 14]. For example, it has been shown that tannins from tea leaves or persimmon have antibacterial effects against Streptococcus mutans , S .…”

Section: Introductionmentioning

confidence: 99%

Antibacterial Effects of Glycyrrhetinic Acid and Its Derivatives on Staphylococcus aureus

et al. 2016

View full text Add to dashboard Cite

Staphylococcus aureus is a major pathogen in humans and causes serious problems due to antibiotic resistance. We investigated the antimicrobial effect of glycyrrhetinic acid (GRA) and its derivatives against 50 clinical S. aureus strains, including 18 methicillin-resistant strains. The minimum inhibitory concentrations (MICs) of GRA, dipotassium glycyrrhizate, disodium succinoyl glycyrrhetinate (GR-SU), stearyl glycyrrhetinate and glycyrrhetinyl stearate were evaluated against various S. aureus strains. Additionally, we investigated the bactericidal effects of GRA and GR-SU against two specific S. aureus strains. DNA microarray analysis was also performed to clarify the mechanism underlying the antibacterial activity of GR-SU. We detected the antimicrobial activities of five agents against S. aureus strains. GRA and GR-SU showed strong antibacterial activities compared to the other three agents tested. At a higher concentration (above 2x MIC), GRA and GR-SU showed bactericidal activity, whereas at a concentration of 1x MIC, they showed a bacteriostatic effect. Additionally, GRA and GR-SU exhibited a synergistic effect with gentamicin. The expression of a large number of genes (including transporters) and metabolic factors (carbohydrates and amino acids) was altered by the addition of GR-SU, suggesting that the inhibition of these metabolic processes may influence the degree of the requirement for carbohydrates or amino acids. In fact, the requirement for carbohydrates or amino acids was increased in the presence of either GRA or GR-SU. GRA and GR-SU exhibited strong antibacterial activity against several S. aureus strains, including MRSA. This activity may be partly due to the inhibition of several pathways involved in carbohydrate and amino acid metabolism.

show abstract

The effect of the flavonol morin on adhesion and aggregation of Streptococcus pyogenes

Cited by 19 publications

References 41 publications

Usnic acid, a lichen secondary metabolite inhibits Group A Streptococcus biofilms

Usnic acid, a lichen secondary metabolite inhibits Group A Streptococcus biofilms

Morin Attenuates Streptococcus suis Pathogenicity in Mice by Neutralizing Suilysin Activity

Antibacterial Effects of Glycyrrhetinic Acid and Its Derivatives on Staphylococcus aureus

Contact Info

Product

Resources

About