Fungal populations and mycotoxins in silage in Assiut and Sohag governorates in Egypt, with a special reference to characteristic Aspergilli toxins

El-Shanawany, A. A.; Mostafa, Mohamed F.; Barakat, Ashraf M.

doi:10.1007/s11046-004-5494-1

Cited by 74 publications

(63 citation statements)

References 25 publications

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“…These results are in agreement with that of authors who did not detect significant OTA levels in maize silage (Richard et al, 2009;El-Shanawany et al, 2005).…”

Section: Discussionsupporting

confidence: 94%

Effects of temperature and incubation time on growth and ochratoxin A biosynthesis by Aspergillus carbonarius and Aspergillus ochraceus in grain-based media

Souza¹,

Pereira²,

Passamani³

et al. 2016

Afr. J. Microbiol. Res.

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Ochratoxin A (OTA) has been frequently found as a food contaminant, and it is considered one of the mycotoxins most harmful to human health. In this context, this study was performed aiming to evaluate mycelial growth and ochratoxin A production from three isolates of Aspergillus carbonarius and Aspergillus ochraceus. The isolates were inoculated on culture media coffee, wheat and maize as well as YES medium and cultivated at 15 and 25°C. Measurement of colony diameters was performed every 48 h for growth description. OTA quantification was made with high-performance liquid chromatography (HPLC) three times (5 th , 15 th and 25 th day). All the isolates presented higher growth rate in YES medium at 25°C; however, growth in every culture media at both tested temperatures was observed. The maize-based medium showed the lowest growth rate. YES medium induced the most OTA production. In grain-based culture media, A. ochraceus isolates produced lower quantities of OTA, reaching a maximum of 9.95 µg/g. However, A. carbonarius isolates produced much higher quantities, showing the A. carbonarius CCDCA10608 isolate, which produced 29.83 µg/g at 15°C in YES medium and 13.06 in wheat-based medium at 15°C. Therefore, among the tested conditions, those more and less favorable to OTA production were recognized.

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“…These results are in agreement with that of authors who did not detect significant OTA levels in maize silage (Richard et al, 2009;El-Shanawany et al, 2005).…”

Section: Discussionsupporting

confidence: 94%

Effects of temperature and incubation time on growth and ochratoxin A biosynthesis by Aspergillus carbonarius and Aspergillus ochraceus in grain-based media

Souza¹,

Pereira²,

Passamani³

et al. 2016

Afr. J. Microbiol. Res.

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“…Mucorales have been reported to be often isolated from silage (41,42,43,44,45,46,47) and are regarded as the aetiological agents of Mucormycosis. However, their capabilities to produce mycotoxins have not been profoundly investigated and little information is available on toxin production by Mucorales.…”

Section: Discussionmentioning

confidence: 99%

Detection of 3-nitropropionic acid and cytotoxicity inMucor circinelloides

et al. 2008

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Mucorales are regarded as the aetiological agents of Mucormycosis. Their capabilities to produce mycotoxins are not profoundly investigated, in contrast to those of the fungi from the generaPenicillium, Aspergillus, orFusarium. The aim of this study was to isolate and identify fungi of the order Mucorales and investigate mycotoxins production. Twelve samples of visibly moulded grass silage and eight samples of damaged whole crop maize silage were analysed. Malt extract agar plates were used for sub cultivation. Three fungal species of the order Mucorales were isolated from grass silage, which were identified by their macro-and micro-morphology asAbsidia corymbifera, Mucor circinelloides andRhizopus stolonifer. The cytotoxicity ofMucor circinelloides extract was analysed using the cytotoxicity test (MTT assay) and the result, showed a low cytotoxicity. Additionally extracts fromAbsidia corymbifera, Mucor circinelloides andRhizopus stolonifer were tested for mycotoxin-production using an LC/MS/MS-based multimycotoxin method. 3-nitropropionic acid was detected in the culture extract ofMucor circinelloides.

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“…A‰atoxins showed the highest incidence rates of occurrence in 22.5z of all samples analyzed. Other mycotoxins were detected in all samples (33). A signiˆcant higher concentration of a‰atoxins was detected in the serum of Egyptian patients with HCC compared to their controls; with two fold increased risk.…”

Section: Genetic Toxicology Of Chemicalsmentioning

confidence: 85%

Genetic Toxicology Research in Developing Countries: Challenges and Possibilities—Egypt as an Example

Anwar

2011

Genes and Environment

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Egypt, as many other developing countries, has several environmental exposure problems. There are exposures to chemical genotoxicants and to lifestyle factors that have been linked to increased risk for cancer. Infections can be associated with cancer development when the environmental factors interact with the infection and lead to the enhancement of the carcinogenic process. Currently, there is a growing interest to genetic toxicology research, the use of diŠerent biomarkers and genetic susceptibility testing, which can contribute eŠectively to risk assessment. Developing countries need to cooperate with developed countries to protect human health from disease determined or in‰uenced by factors in the environment. The national and international research policies should highlight the need to mobilize resources for human resource development, networking, improving research culture, information sharing and pragmatic use of researchˆndings. Exchanging of experience and training is the most vital issues in developing new cadres of people with skills in health research, information and communication, who are needed to address the challenges facing the development of genetic toxicology research and prevention programs. Organizing international meetings and training courses may enforce thisˆeld of research and help to develop cooperative research projects which deal with diŠerent exposure conditions.

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Fungal populations and mycotoxins in silage in Assiut and Sohag governorates in Egypt, with a special reference to characteristic Aspergilli toxins

Cited by 74 publications

References 25 publications

Effects of temperature and incubation time on growth and ochratoxin A biosynthesis by Aspergillus carbonarius and Aspergillus ochraceus in grain-based media

Effects of temperature and incubation time on growth and ochratoxin A biosynthesis by Aspergillus carbonarius and Aspergillus ochraceus in grain-based media

Detection of 3-nitropropionic acid and cytotoxicity inMucor circinelloides

Genetic Toxicology Research in Developing Countries: Challenges and Possibilities—Egypt as an Example

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