Ochratoxin A (OTA) has been frequently found as a food contaminant, and it is considered one of the mycotoxins most harmful to human health. In this context, this study was performed aiming to evaluate mycelial growth and ochratoxin A production from three isolates of Aspergillus carbonarius and Aspergillus ochraceus. The isolates were inoculated on culture media coffee, wheat and maize as well as YES medium and cultivated at 15 and 25°C. Measurement of colony diameters was performed every 48 h for growth description. OTA quantification was made with high-performance liquid chromatography (HPLC) three times (5 th , 15 th and 25 th day). All the isolates presented higher growth rate in YES medium at 25°C; however, growth in every culture media at both tested temperatures was observed. The maize-based medium showed the lowest growth rate. YES medium induced the most OTA production. In grain-based culture media, A. ochraceus isolates produced lower quantities of OTA, reaching a maximum of 9.95 µg/g. However, A. carbonarius isolates produced much higher quantities, showing the A. carbonarius CCDCA10608 isolate, which produced 29.83 µg/g at 15°C in YES medium and 13.06 in wheat-based medium at 15°C. Therefore, among the tested conditions, those more and less favorable to OTA production were recognized.
ResumoPensar a gestão democrática do patrimônio cultural de um lugar, seja qual for sua natureza, suscita um série de discussões, principalmente no que diz respeito a sua legitimidade social, isto por estarem dia-a-dia dialogando com problemas socioeconômicos, pressões imobiliárias e uma série de outros fatores. The present article will mention the case of São Francisco do Sul, Santa Catarina, the economic and social character existing project, ruled by the cultural heritage preservation, as well as the actions carried through for governmental and non governmental institutions with the purpose of democratizating the access to this heritage with emphasis to the work carried out by the Museu Nacional do Mar.
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