Fundamental and applied aspects of analysis of the oxygen metabolisms of phagocytic cells

Zemskov, V. M.; Barsukov, A. A.; Gnatenko, D. A.; Shishkina, N S; Kulikova, A. N.; Козлова, М. Н.

doi:10.1134/s2079086414020091

Cited by 3 publications

(1 citation statement)

References 3 publications

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“…Oxygen metabolism in phagocytic cells was analyzed (on a multiple Synergy 2 SLAD detector-analyzer, Winooski, Vermont, USA) with chemiluminescence (CL), adding luminol or lucigenin used to detect generated reactive oxygen species (ROS) to enhance the chemiluminescence reaction. According to some researchers, it is essential that the oxidation of lucigenin mainly helps identify the extracellular generation of superoxide radical, whereas luminol oxidation mainly identifies intracellular generation [13,14]. The reaction was conducted in wells using 150 μL luminol or lucigenin solution, 25 μL normal human serum (opsonized) zymosan suspension, and 5 μL whole blood of patients.…”

Section: Methodsmentioning

confidence: 99%

Immune Status of Pancreatic Cancer Patients Receiving Cryosurgery

Zemskov

Pronko

Ionkin³

et al. 2019

Medical Sciences

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Cryosurgery used on patients with unresectable pancreatic cancer improved their quality of life, but mainly because of the pain relief. In postoperative patients, multifaceted changes in immunity were found, and the state of the immune system prior to surgery often was a decisive factor to indicate whether further disorders in the postoperative period would develop, or by contrast, it would boost its recovery. Some patients receiving cryosurgery showed immune system imbalance and activation, and of antitumor immunity in particular. It has been suggested that the advisability of immunotropic therapy for specific treatment algorithms should be predicted or the therapy should be suspended at some pathologic stage, and this has been immunologically confirmed. Cryosurgery should be considered as a reasonable alternative to the existing types of surgery for pancreatic cancer or as an essential component of multimodal therapy, consisting of topical cryosurgery, chemotherapy, and immunotropic therapy, to boost antitumor immunity and to discontinue cytoreductive therapy due to its toxic effects.

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Section: Methodsmentioning

confidence: 99%

Immune Status of Pancreatic Cancer Patients Receiving Cryosurgery

Zemskov

Pronko

Ionkin³

et al. 2019

Medical Sciences

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Expertly significant indices of functional activity of bulls’ blood cells

Eremina¹,

Makarskaya²,

Герасимова³

et al. 2022

Industrial, Mechanical and Electrical Engineering

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Immune Diagnostics and Immunotherapy of Burn Sepsis

Kozlova,

Zemskov,

Alekseev

2023

VECS

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The paper analyzes the literature data and authors proper experience in the study of immunopathogenesis and immunodiagnosis of burn sepsis. It argues the issues of effective use of immunocorrection in the complex treatment of severely burned patients.Diagnosis of sepsis after severe burn injury is challenging due to the overlap of signs and clinical manifestations of the hypermetabolic reaction of thermal injury and sepsis. The systemic inflammatory response caused by burns can mimic manifestations of sepsis and complicate its early diagnosis. Considering this, modern immunodiagnostics can serve as an effective tool in identifying damaged key immune markers in burns, determining the severity of immune status disorders in burn disease and the risk of developing septic complications for timely immunocorrection and providing appropriate complex therapy for patients with extensive burns.However, the problem of immunocorrective therapy in severely burned patients remains extremely relevant, debatable and not fully resolved. It is a personalized approach based on immune analysis and clinical recommendations for the complex treatment of burn injury that should be applied in the immunotherapy of burn sepsis to improve the clinical outcomes and, possibly, prevent the development of sepsis in patients with severe burn injury.

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Fundamental and applied aspects of analysis of the oxygen metabolisms of phagocytic cells

Cited by 3 publications

References 3 publications

Immune Status of Pancreatic Cancer Patients Receiving Cryosurgery

Immune Status of Pancreatic Cancer Patients Receiving Cryosurgery

Expertly significant indices of functional activity of bulls’ blood cells

Immune Diagnostics and Immunotherapy of Burn Sepsis

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