Functions of OsWRKY24, OsWRKY70 and OsWRKY53 in regulating grain size in rice

Tang, Jufen; Mei, Enyang; He, Ming; Bu, Qingyun; Tian, Xiaojie

doi:10.1007/s00425-022-03871-w

Cited by 9 publications

(4 citation statements)

References 37 publications

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“… 24 In recent research, it has been reported that OsWRKY24, −53, and −70 were involved in regulation of grain size. 18 , 25 We speculate that the specific expression of OsWRKY24, −53, and −70 in panicles at the heading stage may be related to the regulation of grain size ( Figure 5b ). Moreover, OsWRKY24 and OsWRKY53 play an important role in the regulation of blast resistance.…”

Section: Discussionmentioning

confidence: 99%

Expression identification of three OsWRKY genes in response to abiotic stress and hormone treatments in rice

Li,

Chen,

Zhang

et al. 2023

Plant Signaling & Behavior

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WRKY transcription factors are critical for plant growth, development, and adaptation to stress. This paper focuses on the expression characteristic to abiotic stress and phytohormones of OsWRKY24 , OsWRKY53 , and OsWRKY70 . Three OsWRKY TFs contained two conserved domains and there were multiple cis -elements in response to adversity stress and hormone signaling in their promoters. Real-time PCR analysis revealed their widespread expression in normal tissues during seedling and heading stages. Under various stresses such as darkness, low temperature, salt, and drought, or treatment with hormones like ABA, SA, MeJA, and GA, transcript levels of these genes had changed significantly in wild-type seedlings. The expression level of OsWRKY24 was upregulated by darkness, cold, SA, and MeJA but downregulated by salt, drought, ABA, and GA treatments. The transcripts of OsWRKY53 were induced by darkness, low-temperature, salt, drought, ABA, and JA, while inhibited by SA and GA. In addition, OsWRKY70 expression level was elevated under darkness, low-temperature, SA, and JA but suppressed with salt, drought, ABA, and GA. These findings provide valuable insights into the regulatory mechanisms by which WRKY TFs adapt to stress via plant-hormone signaling.

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Section: Discussionmentioning

confidence: 99%

Expression identification of three OsWRKY genes in response to abiotic stress and hormone treatments in rice

Li,

Chen,

Zhang

et al. 2023

Plant Signaling & Behavior

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“…The differential binding of WRKY53 to the cis ‐element variation in the promoter of SGW5 regulates its differential expression between two parents. Previous study highlighted the important role of WRKY53 in grain size development (Tang et al ., 2022); however, the specific regulatory role of WRKY53 in relation to the natural variation in grain width has not been investigated so far. Previous studies reported that WRKY53 works as a positive regulator for grain size (Tian et al ., 2021), while our findings indicate a high binding affinity of WRKY53 with the SGW5 promoter in the HX354 allele for narrow grain width (Fig.…”

Section: Discussionmentioning

confidence: 99%

The GW5‐WRKY53‐SGW5 module regulates grain size variation in rice

Abbas,

Shalmani,

Zhang

et al. 2024

New Phytologist

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Summary Grain size is a crucial agronomic trait that affects stable yield, appearance, milling quality, and domestication in rice. However, the molecular and genetic relationships among QTL genes (QTGs) underlying natural variation for grain size remain elusive. Here, we identified a novel QTG SGW5 (suppressor of gw5) by map‐based cloning using an F2 segregation population by fixing same genotype of the master QTG GW5. SGW5 positively regulates grain width by influencing cell division and cell size in spikelet hulls. Two nearly isogenic lines exhibited a significant differential expression of SGW5 and a 12.2% increase in grain yield. Introducing the higher expression allele into the genetic background containing the lower expression allele resulted in increased grain width, while its knockout resulted in shorter grain hulls and dwarf plants. Moreover, a cis‐element variation in the SGW5 promoter influenced its differential binding affinity for the WRKY53 transcription factor, causing the differential SGW5 expression, which ultimately leads to grain size variation. GW5 physically and genetically interacts with WRKY53 to suppress the expression of SGW5. These findings elucidated a new pathway for grain size regulation by the GW5‐WRKY53‐SGW5 module and provided a novel case for generally uncovering QTG interactions underlying the genetic diversity of an important trait in crops.

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“…We observed several auxin, brassinosteroid signaling pathway genes such as TUD1,OsDWARF/BRD1, BRD2, OsGSK3 which were well documented as important players in grain size (Hu et al, 2013a; Gao et al, 2019; Qin et al, 2018; Huang et al, 2022), were mis-expressed in clsy3-kd lines. Grain development related MAPK pathway genes (Liu et al, 2021; Xu et al, 2018) and transcription factors (TF) such as OsMADS6, OsMADS29 , OsWRKY53 which were known for starch synthesis, proper cellularization, cell size regulation and programmed cell death also mis-regulated in clsy3-kd lines (Yin and Xue, 2012; Nayar et al, 2013, 2014; Zhang et al, 2010; Tang et al, 2022). Multiple starch, protein, lipid metabolism related genes such as OsSGL, RSS, OsSWEET11, OsFAD2, OsLHT1, OsGAD3 that were important for synthesis and their transport, were mis-regulated in clsy3-kd, further indicating a critical role of OsCLSY3 (Ogawa et al, 2011; Tiwari et al, 2016; Yang et al, 2018b; Peng et al, 2022; Liu et al, 2022b).…”

Section: Discussionmentioning

confidence: 99%

Upstream regulator of genomic imprinting in rice endosperm is a small RNA-associated chromatin remodeler

Pal,

Sundar G,

et al. 2023

Preprint

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Genomic imprinting is observed in endosperm, a placenta-like seed tissue, where transposable elements (TEs) and repeat-derived small(s)RNAs mediate epigenetic changes in plants. In imprinting, uniparental gene expression arises due to parent-specific epigenetic marks on one allele but not on the other. The importance of sRNAs and their regulation in endosperm development or in imprinting is poorly understood in crops. Here we show that a previously uncharacterized CLASSY (CLSY)-family chromatin remodeler named OsCLSY3 is essential for rice endosperm development and imprinting, acting as an upstream player in sRNA pathway. Comparative transcriptome and genetic analysis indicated its endosperm-preferred expression and its paternally imprinted nature. These important features were modulated by RNA-directed DNA methylation (RdDM) of tandemly arranged TEs in its promoter. Upon perturbation of OsCLSY3 in transgenic lines we observed defects in endosperm development and loss of around 70% of all sRNAs. Interestingly, well-conserved endosperm-specific sRNAs (siren) that are vital for reproductive fitness in angiosperms were dependent on OsCLSY3. We also observed many imprinted genes and seed development-associated genes under the control of CLSY3-dependent RdDM. These results support an essential role of OsCLSY3 in rice endosperm development and imprinting, and propose similar regulatory strategies involving CLSY3 homologs among other cereals.

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Functions of OsWRKY24, OsWRKY70 and OsWRKY53 in regulating grain size in rice

Cited by 9 publications

References 37 publications

Expression identification of three OsWRKY genes in response to abiotic stress and hormone treatments in rice

Expression identification of three OsWRKY genes in response to abiotic stress and hormone treatments in rice

The GW5‐WRKY53‐SGW5 module regulates grain size variation in rice

Upstream regulator of genomic imprinting in rice endosperm is a small RNA-associated chromatin remodeler

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