Expression identification of three OsWRKY genes in response to abiotic stress and hormone treatments in rice

Abstract

WRKY transcription factors are critical for plant growth, development, and adaptation to stress. This paper focuses on the expression characteristic to abiotic stress and phytohormones of OsWRKY24 , OsWRKY53 , and OsWRKY70 . Three OsWRKY TFs contained two conserved domains and there were multiple cis -elements in response to adversity stress and hormone signaling in their promoters. Real-time PCR analysis revealed their widespread expression in normal tissues during seedling and heading stages. Under various stresses such as darkness, low temperature, salt, and drought, or treatment with hormones like ABA, SA, MeJA, and GA, transcript levels of these genes had changed significantly in wild-type seedlings. The expression level of OsWRKY24 was upregulated by darkness, cold, SA, and MeJA but downregulated by salt, drought, ABA, and GA treatments. The transcripts of OsWRKY53 were induced by darkness, low-temperature, salt, drought, ABA, and JA, while inhibited by SA and GA. In addition, OsWRKY70 expression level was elevated under darkness, low-temperature, SA, and JA but suppressed with salt, drought, ABA, and GA. These findings provide valuable insights into the regulatory mechanisms by which WRKY TFs adapt to stress via plant-hormone signaling.

Identification of WRKY Family Members and Characterization of the Low-Temperature-Stress-Responsive WRKY Genes in Luffa (Luffa cylindrica L.)

“…In oil palm, EgWRKY59 and EgWRKY65 contribute to similar regulatory mechanisms involving ABA-, SA-, and ROS-mediated signaling pathways during an exposure to drought or other abiotic stresses; these two genes may be useful for enhancing the abiotic stress tolerance of plants. A recent study showed that OsWRKY24 and OsWRKY70 expression levels are upregulated by low temperatures, SA, and MeJA but are downregulated by ABA [ 50 ]. In Dendrobium officinale seedlings, the expression levels of nine DoWRKY genes are significantly affected by cold and MeJA treatments, suggestive of their contributions to stress tolerance [ 51 ].…”

CcNAC6 Acts as a Positive Regulator of Secondary Cell Wall Synthesis in Sudan Grass (Sorghum sudanense S.)

“…A total of 90 Sudan grass plants with the same growth rate were selected for each hormone treatment and divided into 3 replicates. Next, 5 Sudan grass plants were taken as samples at 0, 1, 3, 6, 12, and 24 h after treatment with 150 µM ABA [56], 100 µM JA [57], and 2 µM BRs [30], wrapped in aluminum foil, frozen in liquid nitrogen, and stored at −80 • C.…”

Overexpression of a Ramie (Boehmaeria nivea L. Gaud) Group I WRKY Gene, BnWRKY49, Increases Drought Resistance in Arabidopsis thaliana

“…This viral hijacking of AtWRKY20 spatiotemporally redeployed plant chemical immunity within the leaf [9]. OsWRKY53 was induced by abscisic acid (ABA), jasmonate (JA), salt, drought, darkness, and low temperatures but inhibited by gibberellic acid (GA) and salicylic acid (SA) [10]. The WRKY-TFs in various woody plants were widely involved in responses to biotic and abiotic stresses, as well as in plant growth and development [11].…”