Functionalized DNA nanostructures as scaffolds for guided mineralization

Kim, Francesca; Chen, Tong; Burgess, Trevor; Rasie, Prakash; Selinger, Tim Luca; Greschner, Andrea A.; Rizis, Georgios; Carneiro, Karina M. M.

doi:10.1039/c9sc02811k

Cited by 23 publications

(28 citation statements)

References 55 publications

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“… 10 – 12 These acidic residues are considered to be an important contributor to HA binding and biomineralization induction. 10 , 12 To mimic biomineralization, a number of studies have been performed using poly (aspartic acid) (PASP), 13 (poly)peptides 13 – 15 or other polymers containing Asp 16 as analogs of such mineralization proteins to repair demineralized enamel.…”

Section: Introductionmentioning

confidence: 99%

Two-in-one strategy: a remineralizing and anti-adhesive coating against demineralized enamel

et al. 2020

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Tooth enamel is prone to be attacked by injurious factors, leading to a de/remineralization imbalance. To repair demineralized enamel and prevent pulp inflammation caused by biofilm accumulation, measures are needed to promote remineralization and inhibit bacterial adhesion on the tooth surface. An innovative material, poly (aspartic acid)-polyethylene glycol (PASP-PEG), was designed and synthesized to construct a mineralizing and anti-adhesive surface that could be applied to repair demineralized enamel. A cytotoxicity assay revealed the low cytotoxicity of synthesized PASP-PEG. Adsorption results demonstrated that PASP-PEG possesses a high binding affinity to the hydroxyapatite (HA)/tooth surface. In vitro experiments and scanning electron microscopy (SEM) demonstrated a strong capacity of PASP-PEG to induce in situ remineralization and direct the oriented growth of apatite nanocrystals. Energy dispersive X-ray spectroscopy (EDS), X-ray diffraction analysis (XRD) and Vickers hardness tests demonstrated that minerals induced by PASP-PEG were consistent with healthy enamel in Ca/P ratio, crystal form and surface micro-hardness. Contact angle tests and bacterial adhesion experiments demonstrated that PASP-PEG yielded a strong anti-adhesive effect. In summary, PASP-PEG could achieve dual effects for enamel repair and anti-adhesion of bacteria, thereby widening its application in enamel repair.

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Section: Introductionmentioning

confidence: 99%

Two-in-one strategy: a remineralizing and anti-adhesive coating against demineralized enamel

et al. 2020

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“…SDS-PAGE electrophoresis: Protein samples were characterized using 12% SDS- As a control, we conducted similar experiments with NA nanoribbons and polyaspartic acid (pAsp), a known mineral promoter, instead of amelotin. pAsp is an amorphous mineral carrier that has been previously shown to promote in vitro HAP growth on recombinant amelogenin (rH146), DNA and demineralized dentin through the PILP process [25][26][27]. As observed by AFM and as previously described [28], pAsp assembles into nanospheres in CaP solutions (Supplementary Figure S9).…”

Section: Methodsmentioning

confidence: 57%

Uniaxial Hydroxyapatite Growth on a Self-Assembled Protein Scaffold

Danesi

Athanasiadou

Mansouri

et al. 2021

IJMS

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Biomineralization is a crucial process whereby organisms produce mineralized tissues such as teeth for mastication, bones for support, and shells for protection. Mineralized tissues are composed of hierarchically organized hydroxyapatite crystals, with a limited capacity to regenerate when demineralized or damaged past a critical size. Thus, the development of protein-based materials that act as artificial scaffolds to guide hydroxyapatite growth is an attractive goal both for the design of ordered nanomaterials and for tissue regeneration. In particular, amelogenin, which is the main protein that scaffolds the hierarchical organization of hydroxyapatite crystals in enamel, amelogenin recombinamers, and amelogenin-derived peptide scaffolds have all been investigated for in vitro mineral growth. Here, we describe uniaxial hydroxyapatite growth on a nanoengineered amelogenin scaffold in combination with amelotin, a mineral promoting protein present during enamel formation. This bio-inspired approach for hydroxyapatite growth may inform the molecular mechanism of hydroxyapatite formation in vitro as well as possible mechanisms at play during mineralized tissue formation.

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“…130 DNA was also used to controllably place mineralising groups to achieve controlled growth of calcium phosphate. 131 One of the great potential advantages of the breadboard approach is the possibility for re-use of the template, something that is difficult to achieve with coating and casting. A proof of concept illustration of this approach was recently reported by Sleiman and co-workers.…”

Section: [H2] Templated Assemblymentioning

confidence: 99%

Synthesis and applications of anisotropic nanoparticles with precisely defined dimensions

et al. 2020

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Where a licence is displayed above, please note the terms and conditions of the licence govern your use of this document.When citing, please reference the published version. Take down policyWhile the University of Birmingham exercises care and attention in making items available there are rare occasions when an item has been uploaded in error or has been deemed to be commercially or otherwise sensitive.

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Functionalized DNA nanostructures as scaffolds for guided mineralization

Abstract: Nanopatterned DNA–polyaspartic acid (pAsp) conjugates guide the growth of calcium phosphate minerals in the polymer-induced liquid precursor (PILP) method.

Cited by 23 publications

References 55 publications

Two-in-one strategy: a remineralizing and anti-adhesive coating against demineralized enamel

Two-in-one strategy: a remineralizing and anti-adhesive coating against demineralized enamel

Uniaxial Hydroxyapatite Growth on a Self-Assembled Protein Scaffold

Synthesis and applications of anisotropic nanoparticles with precisely defined dimensions

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