Uniaxial Hydroxyapatite Growth on a Self-Assembled Protein Scaffold

Danesi, Alexander L.; Athanasiadou, Dimitra; Mansouri, Ahmad; Phen, Alina; Neshatian, Mehrnoosh; Holcroft, James W.; Ganß, Bernhard; Carneiro, Karina M. M.

doi:10.3390/ijms222212343

Cited by 4 publications

(3 citation statements)

References 30 publications

(41 reference statements)

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“…Self-assembly of amelogenin controls the oriented and elongated growth of the hydroxyapatite crystals within enamel prisms [ 17 – 19 ]. Use of recombinant amelogenins to form hydroxyapatite crystals has been attempted under several in vitro conditions [ 13 , 20 ]. Although the recombinant amelogenins have been produced by several methods including Escherichia coli- based, yeast-based, and virus expression systems, no methods have been widely adopted due to uncertainty, such as involvement of its phosphorylation [ 21 ].…”

Section: Introductionmentioning

confidence: 99%

Regaining enamel color quality using enamel matrix derivative

et al. 2023

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This study aimed to demonstrate and compare the accuracy of tooth shade selection due to the remineralized enamel crystal with enamel matrix derivative (EMD) in vitro. Etched enamel slices were immersed in four types of mineralization buffers for 16 h. Sodium fluoride (NaF) was added to final concentrations of 1–100 ppm with the mineralization buffer that demonstrated the highest mineralization efficiency. EMD was added to the mineralization buffer containing NaF to see if it has any remineralization capacities. The remineralized enamel crystal was analyzed by SEM and XRD. The tooth shade was evaluated by CIE L*a*b*. The results showed that, without NaF, plate-like nanocrystals were formed on the enamel surface, but with NaF, needle-like nanocrystals were formed. By adding EMD, a layer of well-compacted hydroxyapatite crystals was successfully precipitated onto the natural enamel surface. No significant differences were observed in the L* value of the mineralization surface pre-etching and after mineralization buffer containing NaF and EMD. A new method has been developed to recover the color quality of enamel, as well as to mineralize the tooth enamel by constructing hydroxyapatite crystals with mineralization buffers containing NaF and EMD on the etched tooth surface.

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Section: Introductionmentioning

confidence: 99%

Regaining enamel color quality using enamel matrix derivative

et al. 2023

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“…Furthermore, the complications after surgery experienced by the graft recipient are another problem in this type of grafting (4). Nowadays, bone regeneration technologies and tissue engineering (TE) methods have revolutionized and are promising in the regeneration of masticatory teeth and the repair of jaw defects (5)(6)(7)(8)(9).…”

Section: Introductionmentioning

confidence: 99%

On the osteogenic differentiation of dental pulp stem cells by a fabricated porous nano-hydroxyapatite scaffold loaded with sodium fluoride

Arab,

Bahraminasab,

Asgharzade

et al. 2024

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In this study, after the extraction of nano-hydroxyapatite (n-HA) powder from carp bone waste, porous n-HA scaffolds were fabricated by a sintering process and subsequently loaded with different amounts of sodium fluoride (NaF) through immersion in NaF suspensions for 10, 7.5, and 5 minutes. The NaF-loaded scaffolds were then examined for their structural properties, loading and release profile, cytotoxicity and osteogenic effect on dental pulp stem cells at the level of RNA and protein expression. Scanning electron microscopy (SEM) images showed that the scaffolds were porous and had rough surfaces, and NaF could be successfully applied. The NaF loaded on the n-HA scaffolds was 6.43, 4.50, and 1.47 mg, respectively at 10-, 7.5-, and 5-minutes immersion in the NaF suspensions. The NaF release rate was fast during the first hour in all groups (38.6%, 29.7%, and 25.7% for 10, 7.5, and 10 min, respectively), and decreased dramatically thereafter, especially after 6h, indicating a slow detachment of NaF. The results of the cytotoxicity assay rejected any toxicity of the fabricated scaffolds on DPSCs, and the cells could adhere to the scaffold surfaces with enlarged morphology. The results obtained showed no effect on osteogenic differentiation at the protein level. Nevertheless, this effect was observed at the gene level.

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“…This bio-inspired approach for hydroxyapatite growth study can be informative in deepening the comprehension of the molecular mechanism of hydroxyapatite formation in vitro as well as in understanding the mechanisms at play during mineralized tissue formation. This is of crucial importance for the development of artificial biomaterials capable of mineralized tissue regeneration [ 5 ]. The fibrillization of peptides is relevant to numerous diseases related to the deposition of amyloids.…”

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confidence: 99%