Functionalization of MWNTs with Hyperbranched PEI for Highly Selective Isolation of BSA

Abstract

Cationic hyperbranched BPEI was immobilized on the surface of MWNTs via electrostatic interactions between the positively charged protonated amines within the polymer and the carboxyl groups on the chemically oxidized MWNT surface. The functionalized BPEI-MWNTs were characterized by FT-IR, TGA, TEM and surface charge analysis, and it was used as a bio-sorbent for the adsorption of proteins. CD spectra showed no conformational change of BSA during the adsorption/desorption process. A dynamic adsorption capacity of 167 mg · g⁻¹ for BSA was achieved. With a sample volume of 2.0 mL, an enrichment factor of 10 was obtained along with an adsorption efficiency of 100%, a recovery of 100%, a sampling frequency of 10 h⁻¹ and a RSD of 2.6% at 25 µg · mL⁻¹ BSA.

Polyethyleneimine–iron phosphate nanocomposite as a promising adsorbent for the isolation of DNA

“…These bands are also clearly identified in the spectrum of PEI-FePO 4 nanocomposites. After the modification of PEI, the absorption bands at 3300-3600 cm À 1 , 2850 cm À 1 and 2919 cm À 1 , which are the characteristic stretching vibrations of N-H, vibration of -CH 3 and -CH 2 of PEI, are all observed in the spectrum of PEI-FePO 4 nanocomposites [24]. These observations well suggest the successful immobilization of PEI onto the FePO 4 nanoparticles.…”

Architecture, Assembly, and Emerging Applications of Branched Functional Polyelectrolytes and Poly(ionic liquid)s

“…107 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 In another study, hyperbranched polyethyleneimine was immobilized on the surface of multi-walled nanotubes (MWNTs) via electrostatic interactions between the positively charged protonated amines within the polymer and the carboxyl groups on the chemically oxidized MWNT surface. 109 The branched polyelectrolyte not only provides many sites for the location of protein but also effectively reduces the lateral repulsion of Another important category of hyperbranched polyelectrolytes is hyperbranched conjugated polyelectrolytes (HCPEs), introduced as novel optical, electronic and magnetic materials. 111 These materials exhibit good solubility and excellent processability.…”

The Interaction Between Amphiphilic Polymer Materials and Guest Molecules: Selective Adsorption and Its Related Applications

“…As bovine serum albumin (BSA) possessed a negative charge, whereas hemoglobin (Hb) possessed a positive charge using ultrapure water as a medium, BSA was efficiently adsorbed by PDDA and separated from the mixture . Similar results were obtained when PDDA was replaced by another cationic amphiphilic polymer, hyperbranched PEI . The electrostatic interaction between proteins and amphiphilic polymers plays a key role in the separation of proteins by these ionic amphiphilic polymers.…”