Functional regeneration of ligament-bone interface using a triphasic silk-based graft

Li, Hongguo; Fan, Jiabing; Sun, Liguo; Liu, Xincheng; Cheng, Pengzhen; Fan, Hongbin

doi:10.1016/j.biomaterials.2016.08.012

Cited by 52 publications

(50 citation statements)

References 37 publications

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“…At the end of the study, it was observed how the cells totally colonized the spaces between the braiding fibers. This good affinity of the cells for the SGFBs is in concordance with other silk based materials used in tissue repair and more specifically in tendon and ligament healing studies …”

Section: Results and Discusionsupporting

confidence: 82%

“…Other authors have previously worked in tissue engineering of ACL and different types of fibroin‐based scaffolds have been implanted in rabbits with this aim, however, our SGF‐based scaffold improved the values of breaking force stated in most of the previous works.…”

Section: Results and Discusionmentioning

confidence: 75%

“…The complexity of this type of scaffolds derived from knitted SF has led to the co‐culture of the different cell types involved in the complete regeneration, both of the ligament and the insertions in the bone tissue. In this sense, Li et al . have developed and tested in vivo three‐phase scaffolds with bone marrow mesenchymal stem cells, chondrocytes and osteoblasts respectively seeded on the ligament, cartilage and bone region of triphasic silk grafts.…”

Section: Introductionmentioning

confidence: 99%

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Potential use of silkworm gut fiber braids as scaffolds for tendon and ligament tissue engineering

et al. 2019

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Tendon and ligament tissue engineering require scaffolds for the treatment of various conditions in the medical field. These must meet requirements such as high tensile strength, biocompatibility, fast and stable repair and a rate of degradation that allows the repair of the damaged tissue. In this work, we propose the use of silkworm gut fiber braids as materials to temporarily replace and repair this type of tissues. The mechanical characterization of the braids made with different number of silk gut fibers is provided, as well as a descriptive analysis of the proliferation and adhesion of cultures of adult human mesenchymal stem cells from bone marrow and fibroblasts (L929) on the braids. As expected, the breaking force increases linearly in the scaffold with the number of fibers, thus being a parameter adaptable to the specific requirements of the tissue to repair and the animal model of study. On the other hand, in all of the cases studied, the values obtained for the elastic modulus of the hydrated fibers were in the range of the ones reported for various human tendons and ligaments. Moreover, the scaffold demonstrated excellent biocompatibility in vitro, allowing the adhesion and proliferation, in the same culture conditions, of the two cell types studied, therefore posing as an ideal candidate to be employed in future in vivo studies that allow elucidating its behavior in the articular environment or extra‐articular tendinous areas. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res B Part B: 2019. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B: 2209–2215, 2019.

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Section: Results and Discusionsupporting

confidence: 82%

Section: Results and Discusionmentioning

confidence: 75%

Section: Introductionmentioning

confidence: 99%

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Potential use of silkworm gut fiber braids as scaffolds for tendon and ligament tissue engineering

et al. 2019

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“…This has motived the development of tissue engineering (TE) strategies for ligament regeneration [8,9]. A number of studies have attempted to engineer stable fibrocartilaginous tissue or a mineral gradient to interface between the calcified region and the main body of the engineered ligament [10][11][12][13][14], although the ideal solution has yet to be identified.…”

Section: Introductionmentioning

confidence: 99%

Modulating microfibrillar alignment and growth factor stimulation to regulate mesenchymal stem cell differentiation

et al. 2017

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The ideal tissue engineering (TE) strategy for ligament regeneration should recapitulate the bonecalcified cartilage -fibrocartilage -soft tissue interface. Aligned electrospun-fibers have been shown to guide the deposition of a highly organized extracellular matrix (ECM) necessary for ligament TE.However, recapitulating the different tissues observed in the bone-ligament interface using such constructs remains a challenge. This study aimed to explore how fiber alignment and growth factor stimulation interact to regulate the chondrogenic and ligamentous differentiation of mesenchymal stem cells (MSCs). Without growth factor stimulation, MSCs on aligned-microfibers showed higher levels of tenomodulin (TNMD) and aggrecan gene expression compared to MSCs on randomlyoriented fibers. MSCs on aligned-microfibers stimulated with transforming growth factor β3 (TGFβ3) formed cellular aggregates and underwent robust chondrogenesis, evidenced by increased type II collagen expression and sulphated glycosaminoglycans (sGAG) synthesis compared to MSCs on randomly-oriented scaffolds. Bone morphogenetic protein 2 (BMP2) and type I collagen gene expression were higher on randomly-oriented scaffolds stimulated with TGFβ3, suggesting this substrate was more supportive of an endochondral phenotype. In the presence of connective tissue growth factor (CTGF), MSCs underwent ligamentous differentiation, with increased TNMD expression on aligned compared to randomly aligned scaffolds. Upon sequential growth factor stimulation, MSCs expressed types I and II collagen and deposited higher overall levels of collagen compared to scaffolds stimulated with either growth factor in isolation. These findings demonstrate that modulating the alignment of microfibrillar scaffolds can be used to promote either an endochondral, chondrogenic, fibro-chondrogenic or ligamentous MSC phenotype upon presentation of appropriate biochemical cues.

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“…33,34 To test the osseointegration Fig. 4 The full-survey-scan XPS spectrum of 3D rGO/PPY based scaffolds (a), and the P 2p spectra of 3D rGO/PPY/CPP10 and 3D rGO/ PPY/CPP20 (b) composite scaffolds, respectively.…”

Section: Bioactivity Evaluation Of the Cpp-modied 3d Rgo/ppy Scaffoldmentioning

confidence: 99%

Enhancing the proliferation of MC3T3-E1 cells on casein phosphopeptide-biofunctionalized 3D reduced-graphene oxide/polypyrrole scaffolds

Jie

Song

et al. 2017

RSC Adv.

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In bone tissue engineering, the 3D macro-scaffold plays an indispensable role as a matrix for cell proliferation, differentiation and tissue formation, for which a bioactive surface is required. Previously, we successfully prepared the 3D macro-reduced graphene oxide/polypyrrole (3D rGO/PPY) scaffold; in the present study, by using casein phosphopeptide (CPP) as a bioactive molecule, we have developed a 3D rGO/PPY/CPP composite scaffold through a simple, but low-cost electrostatic self-assembly method and have explored the application of the composite scaffold in bone tissue engineering. The results have indicated that the CPP successfully modified the backbone of the scaffold, and demonstrated that the developed 3D rGO/PPY/CPP scaffold has excellent hydrophilic behavior and water uptake performance, much better than that of 3D rGO/PPY. In the biomimetic mineralization experiment, the CPP-modified matrix, particularly 3D rGO/PPY/CPP20, could promote the rapid formation of hydroxyapatite in simulated body fluid solution on just the 1 st soaking day. On co-culturing with the MC3T3-E1 cells, the 3D rGO/PPY/CPP20 kept the cells at a higher proliferation state of 5.07 times that of the control group, superior to that of the 3D rGO/PPY/CPP10 (3.88 times) and the 3D rGO/PPY group (2.07 times). The excellent osteoblastic performance of the 3D rGO/PPY/CPP20 composite scaffold can be attributed to the good biological properties of CPP, and the unique 3D macro-structure with a high specific surface area. Our findings suggest that the 3D rGO/PPY/CPP20 can be considered as an attractive scaffold for bone healing and regeneration in the future.

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Functional regeneration of ligament-bone interface using a triphasic silk-based graft

Cited by 52 publications

References 37 publications

Potential use of silkworm gut fiber braids as scaffolds for tendon and ligament tissue engineering

Potential use of silkworm gut fiber braids as scaffolds for tendon and ligament tissue engineering

Modulating microfibrillar alignment and growth factor stimulation to regulate mesenchymal stem cell differentiation

Enhancing the proliferation of MC3T3-E1 cells on casein phosphopeptide-biofunctionalized 3D reduced-graphene oxide/polypyrrole scaffolds

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