Functional dye as a comonomer in a water‐soluble polymer

Zhang, Wenwen; Vinueza, Nelson R.; Datta, Preeta; Michielsen, Stephen

doi:10.1002/pola.27592

Cited by 23 publications

(12 citation statements)

References 19 publications

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“…In vitro studies have been carried out to investigate the antibacterial effects of Cu ions from metallic copper surface [28] and Cu-containing nanoparticles. [12] Although many studies have reported the bactericidal mechanism of antibacterial nanomaterials and copolymers in the past few years, [29–31] there have been very few reports about comprehensive mechanisms of the antibacterial effect of Cu ions. In the present study, Ti6Al4V5Cu with antibacterial activity was used to understand the antibacterial mechanism.…”

Section: Discussionmentioning

confidence: 99%

Toward a Molecular Understanding of the Antibacterial Mechanism of Copper‐Bearing Titanium Alloys against Staphylococcus aureus

Zhu

et al. 2015

Adv Healthcare Materials

145

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The antibacterial mechanism of the Cu-containing materials has not been fully understood although such understanding is crucial for the sustained clinical use of Cu-containing antibacterial materials such as bone implants. The aim of this study is to investigate the molecular mechanisms by which the Gram-positive Staphylococcus aureus (S.aureus) is inactivated through Cu-bearing titanium alloys (Ti6Al4V5Cu). Cu ions released from the alloys were found to contribute to lethal damage of bacteria. They destroyed the permeability of the bacterial membranes, resulting in the leakage of reducing sugars and proteins from the cells. They also promoted the generation of bacteria-killing reactive oxygen species (ROS). The ROS production was confirmed by several assays including fluorescent staining of intracellular oxidative stress, detection of respiratory chain activity, and measurement of the levels of lipid peroxidation, catalase and glutathione. Furthermore, the released Cu ions showed obvious genetic toxicity by interfering the replication of nuc (species-specific) and 16SrRNA genes, but with no effect on the genome integrity. All of these effects lead to the antibacterial effect of Ti6Al4V5Cu. Collectively, our work reconciles the conflicting antibacterial mechanisms of Cu-bearing metallic materials or nanoparticles reported in the literature, as well as highlight the potential use of Ti6Al4V5Cu alloys in inhibiting bacterial infections.

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Section: Discussionmentioning

confidence: 99%

Toward a Molecular Understanding of the Antibacterial Mechanism of Copper‐Bearing Titanium Alloys against Staphylococcus aureus

Zhu

et al. 2015

Adv Healthcare Materials

145

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“…Fabrication of thin polymeric films has been of considerable importance in both industrial and fundamental research due to their potential applications in various areas, such as packaging, protective coatings, modern microelectronics, smart actuators, battery separators, antibacterial and antifouling films, biocompatible membranes for drug delivery, or tissue engineering . Among the methods used to design polymeric films, layer‐by‐layer (LbL) assembly strategy is very attractive due to its versatility and the large number of building‐blocks (synthetic and natural polyelectrolytes, inorganic nanoparticles, biomolecules, dyes, etc.…”

Section: Introductionmentioning

confidence: 99%

Sorption/release of diclofenac sodium in/from free‐standing poly(acrylic acid)/poly(ethyleneimine) multilayer films

Ghiorghiță¹,

Bucătariu²,

Drăgan³

2016

J of Applied Polymer Sci

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Polyelectrolyte multilayer films deposited onto various substrates have been used extensively as drug delivery systems. However, little attention has been paid to the release of drugs from free‐standing polymeric films. Herein, we report the construction of thermal crosslinked free‐standing poly(acrylic acid) (PAA)/branched poly(ethyleneimine) (PEI) multilayer films composed of 25 double layers [(PAA/PEI)25] and their use in sorption/release of diclofenac sodium (DS). The (PAA/PEI)25 multilayer films were characterized by scanning electron microscopy, potentiometric titrations and Fourier transform infrared spectroscopy, while the sorption/release of DS was monitored by UV–Vis spectroscopy. The DS sorption equilibrium data were fitted with five isotherm models (Langmuir, Freundlich, Sips, Dubinin–Radushkevich, and Temkin). The maximum equilibrium sorption capacity, qm, given by the Langmuir model was 32.42 mg DS/g. The Korsmeyer–Peppas semiempirical equation showed that the release of DS from the free‐standing (PAA/PEI)25 films proceeded by pseudo‐Fickian diffusion, irrespective of the releasing media. © 2016 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2016, 133, 43752.

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“…Development of protein therapeutics, including enzymes, antibodies, and cytokines, has attracted great attention from both academia and pharmaceutical industry [1][2][3][4][5][6][7][8][9][10]. Chinese hamster ovary (CHO) cells are widely used as the mammalian hosts for expression and production of therapeutic recombinant proteins due to the characteristics of high product quality, safety, and adaptability of suspension culture [11].…”

Section: Introductionmentioning

confidence: 99%

Cell growth stimulating effect of Ganoderma lucidum spores and their potential application for Chinese hamster ovary K1 cell cultivation

Ding

Zhong

et al. 2016

Bioprocess Biosyst Eng

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In this work, water-soluble extracts of Ganoderma lucidum spores (Gls), a Chinese medicinal herb that possesses cell growth stimulating function, were found to be an effective growth factor for Chinese hamster ovary (CHO) cell cultivation. The Gls extract was prepared and supplemented to CHO K1 cell culture media with various serum levels. Our results obtained from both the static culture and the spinner-flask suspension culture showed that use of small-amount Gls extract effectively promoted cell growth and suppressed cell apoptosis induced by serum deprivation with normal cell cycle maintained in a low-serum medium. The low-serum medium containing 1 % (v/v) fetal bovine serum (FBS) and 0.01 % (w/v) Gls extract showed a comparable performance on both cell growth and fusion protein productivity with the conventional CHO culture medium containing 10 % (v/v) FBS and a commercial serum-free medium. This is the first study of the potential of Gls extracts for use as an alternative cell growth factor and nutrient for CHO cells. The findings have presented a new approach to economic cultivation of CHO cells for therapeutic protein production.

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Functional dye as a comonomer in a water‐soluble polymer

Cited by 23 publications

References 19 publications

Toward a Molecular Understanding of the Antibacterial Mechanism of Copper‐Bearing Titanium Alloys against Staphylococcus aureus

Toward a Molecular Understanding of the Antibacterial Mechanism of Copper‐Bearing Titanium Alloys against Staphylococcus aureus

Sorption/release of diclofenac sodium in/from free‐standing poly(acrylic acid)/poly(ethyleneimine) multilayer films

Cell growth stimulating effect of Ganoderma lucidum spores and their potential application for Chinese hamster ovary K1 cell cultivation

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