Cell growth stimulating effect of Ganoderma lucidum spores and their potential application for Chinese hamster ovary K1 cell cultivation

Abstract

In this work, water-soluble extracts of Ganoderma lucidum spores (Gls), a Chinese medicinal herb that possesses cell growth stimulating function, were found to be an effective growth factor for Chinese hamster ovary (CHO) cell cultivation. The Gls extract was prepared and supplemented to CHO K1 cell culture media with various serum levels. Our results obtained from both the static culture and the spinner-flask suspension culture showed that use of small-amount Gls extract effectively promoted cell growth and suppressed cell apoptosis induced by serum deprivation with normal cell cycle maintained in a low-serum medium. The low-serum medium containing 1 % (v/v) fetal bovine serum (FBS) and 0.01 % (w/v) Gls extract showed a comparable performance on both cell growth and fusion protein productivity with the conventional CHO culture medium containing 10 % (v/v) FBS and a commercial serum-free medium. This is the first study of the potential of Gls extracts for use as an alternative cell growth factor and nutrient for CHO cells. The findings have presented a new approach to economic cultivation of CHO cells for therapeutic protein production.

Enhancing clinical safety in bioengineered-root regeneration: The use of animal component-free medium

“…Previous studies using low serum and serum-free medium for cell culture have shown that low fetal bovine serum contains fewer risk components and requires less time, labor, and laboratory space for expanding cells. However, compared with animal component-free medium, the use of low serum medium in clinical applications will still bring many uncertain risks [ 59 , 60 ]. Additionally, the hDPSCs/hTDM complexes cultured in the three media all regenerated dentin-like, pulp-like, and fibrous-like tissue, according to histomorphological analysis of our study, which indicated that ACF medium can substitute SCM for the pre-transplantation culture of hDPSCs/hTDM complexes in tissue engineering of bioengineered-root regeneration in vivo .…”

Ganoderma lucidum Spore Polysaccharide Inhibits the Growth of Hepatocellular Carcinoma Cells by Altering Macrophage Polarity and Induction of Apoptosis

“…G. lucidum spores are microscopic and are ejected from the cap during growth and maturation. These germ cells have all of G. lucidum genetically active substances [8]. Modern pharmacological studies have shown that G. lucidum spores have antitumor effects, increase immune regulation, lower blood sugar and lipid, increase anti-inflammatory and antihypoxia ability, and scavenge free radicals [9].…”