Silver-loaded maifanite prepared at different particle sizes (60 mesh~200 mesh) and light calcining temperatures (100°C~800°C) of maifanite, different AgNO3solution concentrations (0.025 mol•L-1~0.300 mol•L-1) and pH values (2~7) were researched, simultaneously antibacterial ability of silver-loaded maifanite was investigated. Results indicate that the appropriate conditions of preparing silver-loaded maifanite are that particle size is 200 mesh, light calcining temperature is 100°C, AgNO3concentration is 0.1 mol•L-1at pH=7, and then the silver-loading amount of maifanite gained at this conditions is 1.27wt%; antimicrobial ability of silver-loaded maifanite decreases with increase of light calcining temperature.