Screening of the Bacterium for Chlorobenzene Degradation and its Enzymatic Properties

Abstract

A dominant bacterium (LW13) for the degradation of chlorobenzene was selected from maturity sludge in a novel combined bio-filter polluted by chlorobenzene gas. Based on the morphological characteristics observation, physio-biochemical characteristics and 16S rDNA sequence homology analysis, strain LW13 was identified as Lysinibacillus fusiformis. Crude enzyme from the fermentation was extracted and their enzymatic properties were also investigated. Results showed that the degradation enzyme produced by the bacteria belong to extracellular enzymes. The purity of the enzyme was determined by SDS-PAGE gel electrophoresis and the molecular weight was found to be 52 kDa. The optimum pH value was about 8.0 with the optimum temperature of 45° C. Throughout the purification process, 85-fold of enzyme purification was achieved with the recovery of 20.69%.