Functional and morphological studies of in vivo vascularization of 3D-bioprinted human fat grafts

Amoroso, Matteo; Apelgren, Peter; Säljö, Karin; Montelius, Mikael; Orrhult, Linnea Strid; Engström, Mona; Gatenholm, Paul; Kölby, Lars

doi:10.1016/j.bprint.2021.e00162

Cited by 11 publications

(10 citation statements)

References 30 publications

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“…Previous studies showed that the TNC micromilieu subsidizes chondrogenesis and adipose tissue [50,51]. Although studies on other cell types or tissues have not yet been performed, the present findings suggest that from a biological standpoint, TNC likely offers a favorable environment for any cell type or tissue.…”

Section: Tablementioning

confidence: 48%

“…Although intended for a shorter time perspective, this principle has been previously applied to biodegradable bioinks. Tunicate nanocellulose has previously been used as a scaffold in in vivo studies using nude mice [50][51][52]. In all these studies, TNC was mixed with alginate and crosslinked with Calcium ions prior to implantation.…”

Section: Tablementioning

confidence: 99%

“…The penetrating neovascularization evident in the TNC constructs is highly interesting and will potentially bring tissue engineering a step closer to clinical translation. To address this, we recently investigated vascularization longitudinally over 3 months using magnetic resonance imaging [50,51]. To further evaluate this key issue, our next study will quantify the vascular structures and investigate the driving forces of vascularization (e.g., growth factors).…”

Section: Tablementioning

confidence: 99%

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Biomaterial and biocompatibility evaluation of tunicate nanocellulose for tissue engineering

Apelgren

Sämfors

Säljö

et al. 2022

Biomaterials Advances

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Section: Tablementioning

confidence: 48%

Section: Tablementioning

confidence: 99%

Section: Tablementioning

confidence: 99%

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Biomaterial and biocompatibility evaluation of tunicate nanocellulose for tissue engineering

Apelgren

Sämfors

Säljö

et al. 2022

Biomaterials Advances

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“…Liver cells and induced pluripotent stem cells (iPSCs) have been printed and maintained in alginate/NFC scaffolds [50,52] and this combination was reported to be suitable for keeping the pluripotency of iPSCs that was later used to generate cartilage tissue [53]. In the recent studies, combination of tunicate-derived NFC with alginate was used to 3D bioprint human ear chondrocytes and autologous micro-fat for pre-clinical applications [13,54]. Our data shows capabilities of this bioink mixture to diffuse wide-range of small molecules from 3 to70 kDa, which confirms its ability to exchange nutrients, waste and oxygen between the cells and outer environment.…”

Section: Discussionmentioning

confidence: 99%

Adipose-derived stromal cells preserve pancreatic islet function in a transplantable 3D bioprinted scaffold

Abadpour

Niemi

Orrhult

et al. 2022

Preprint

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Intra-portal islet transplantation is the method of choice for treatment of insulin dependent type 1 diabetes, but its outcome is hindered by limited islet survival due to the immunological and metabolic stress post transplantation. Adipose-derived stromal cells (ASCs) promise to improve significantly the islet micro-environment but an efficient long-term delivery method has not been achieved. We therefore explore the potential of generating ASC enriched islet transplant structure by 3D bioprinting. Here, we fabricate a double-layered 3D bioprinted scaffold for islets and ASCs by using alginate-nanofibrillated cellulose bioink. We demonstrate the diffusion properties of the scaffold and report that human ASCs increase the islet viability, preserve the endocrine function, and reduce pro-inflammatory cytokines secretion in vitro. Intraperitoneal implantation of the ASCs and islets in 3D bioprinted scaffold improve the long-term function of islets in diabetic mice. Our data reveals an important role for ASCs on the islet micro-environment. We suggest a novel cell therapy approach of ASCs combined with islets in a 3D structure with a potential for clinical beta cell replacement therapies at extrahepatic sites.

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“…In 2019, Ocean TuniCell AS in Norway successfully produced tunicate nanocellulose on a large-scale based on microfluidization combined with different pretreatment technologies, such as TEMPO-mediated oxidation, carboxymethylation, and enzymatic treatment. Right now, it has commercialized this unique animal nanocellulose by developing them in to TUNICELL 3D-bioinks [ 173 ].…”

Section: Industrialization Of Nanocellulose Production Worldwidementioning

confidence: 99%

Emerging Food Packaging Applications of Cellulose Nanocomposites: A Review

Zhang

Zhong

et al. 2022

Polymers

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Cellulose is the most abundant biopolymer on Earth, which is synthesized by plants, bacteria, and animals, with source-dependent properties. Cellulose containing β-1,4-linked D-glucoses further assembles into hierarchical structures in microfibrils, which can be processed to nanocellulose with length or width in the nanoscale after a variety of pretreatments including enzymatic hydrolysis, TEMPO-oxidation, and carboxymethylation. Nanocellulose can be mainly categorized into cellulose nanocrystal (CNC) produced by acid hydrolysis, cellulose nanofibrils (CNF) prepared by refining, homogenization, microfluidization, sonification, ball milling, and the aqueous counter collision (ACC) method, and bacterial cellulose (BC) biosynthesized by the Acetobacter species. Due to nontoxicity, good biodegradability and biocompatibility, high aspect ratio, low thermal expansion coefficient, excellent mechanical strength, and unique optical properties, nanocellulose is utilized to develop various cellulose nanocomposites through solution casting, Layer-by-Layer (LBL) assembly, extrusion, coating, gel-forming, spray drying, electrostatic spinning, adsorption, nanoemulsion, and other techniques, and has been widely used as food packaging material with excellent barrier and mechanical properties, antibacterial activity, and stimuli-responsive performance to improve the food quality and shelf life. Under the driving force of the increasing green food packaging market, nanocellulose production has gradually developed from lab-scale to pilot- or even industrial-scale, mainly in Europe, Africa, and Asia, though developing cost-effective preparation techniques and precisely tuning the physicochemical properties are key to the commercialization. We expect this review to summarise the recent literature in the nanocellulose-based food packaging field and provide the readers with the state-of-the-art of this research area.

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Functional and morphological studies of in vivo vascularization of 3D-bioprinted human fat grafts

Cited by 11 publications

References 30 publications

Biomaterial and biocompatibility evaluation of tunicate nanocellulose for tissue engineering

Biomaterial and biocompatibility evaluation of tunicate nanocellulose for tissue engineering

Adipose-derived stromal cells preserve pancreatic islet function in a transplantable 3D bioprinted scaffold

Emerging Food Packaging Applications of Cellulose Nanocomposites: A Review

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