Functional Analysis of SBPase Gene Promoter in Transgenic Wheat under Abiotic Stresses

Alotaibi, Saqer S.; Al-Yasi, Hatim M.; El-Shehawi, Ahmed M.; Gaber, Ahmed; Hassan, Mohamed; Aljuaid, Bandar S.; Simkin, Andrew J.; Raines, Christine A.

doi:10.3923/biotech.2019.15.23

Cited by 7 publications

(6 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Taking into account the different fusion genes, all is similar to the results explained in Mukherjee et al , in which the rubisco small subunit gene promoter was isolated from wheat and hence, drove excellent GUS expression in the green tobacco tissues, beyond roots, conversely, its activity was not studied through various stages of growth ( Mukherjee et al, 2015 ). Moreover, the obtained results agree with Alotaibi et al, 2018 , Alotaibi et al, 2019 ) studies, as the same promoters of both the B. distachyon SBPase and FBPA genes were efficient in facilitating GUS transgene expression in wheat leaves. In conclusion, these two Brachypodium promoters of the SBPase and FBPA enzymes were validated as robust genetic tools of choice in plant genetic engineering towards guiding the expression of the transgenic of interest, specifically in mesophyll cells.…”

Section: Discussionsupporting

confidence: 90%

Developing specific leaf promoters tools for genetic use in transgenic plants towards food security

Alotaibi

2021

Saudi Journal of Biological Sciences

View full text Add to dashboard Cite

Significant yields enrichments are necessitated for meeting the rapid global growth population together with the expected demanding for food, particularly major crops. Photosynthesis improvement is an unexploited opportunity in research on improving crop yields. However, the lack of sufficient molecular promoters tools leads to the need to explore and analyze native leaf-specified promoters for manipulating photosynthesis activities in plants. Two B. distachyon promoters, sedoheptulose-1, 7-bisphosphatase (SBPase) and fructose-1, 6-bisphosphate aldolase (FBPA), were isolated and cloned into an expression vector upstream of the eYFP reporter gene. The results demonstrate that both promoters actively function in N. benthamiana leaves in both agro-transiently assays, successfully regulating expression specifically to leaf-tissues. Exploring these active promoters could potentially provide new well genetic tools for any transgene expression in plants or leaves to genetically manipulate photosynthesis for yield improvement.

show abstract

Section: Discussionsupporting

confidence: 90%

Developing specific leaf promoters tools for genetic use in transgenic plants towards food security

Alotaibi

2021

Saudi Journal of Biological Sciences

View full text Add to dashboard Cite

show abstract

“…Over the last few decades, agricultural research has adopted technologies, such as genetic engineering and, more recently, 'genome editing' to improve traits in key crops [182][183][184][185][186]. There have been recent advances in the tools available to carry out this work, including vectors for multiple gene insertions [187][188][189][190][191] and tissue-specific promoters [192][193][194][195][196][197]. The generation of parthenocarpic fruit via genetic manipulation brings its own challenges.…”

Section: Discussionmentioning

confidence: 99%

Fruit Development in Sweet Cherry

Vignati

Lipska

Dunwell

et al. 2022

Plants

View full text Add to dashboard Cite

Fruits are an important source of vitamins, minerals and nutrients in the human diet. They also contain several compounds of nutraceutical importance that have significant antioxidant and anti-inflammatory roles, which can protect the consumer from diseases, such as cancer, and cardiovascular disease as well as having roles in reducing the build-up of LDL-cholesterol in blood plasma and generally reduce the risks of disease and age-related decline in health. Cherries contain high concentrations of bioactive compounds and minerals, including calcium, phosphorous, potassium and magnesium, and it is, therefore, unsurprising that cherry consumption has a positive impact on health. This review highlights the development of sweet cherry fruit, the health benefits of cherry consumption, and the options for increasing consumer acceptance and consumption.

show abstract

“…Agricultural research has adopted key technologies such as genetic engineering and genome editing to improve identifiable traits in crops [30,350,351], and new tools have been developed, including vectors multiple gene insertion [352][353][354][355][356] and tissue-specific promoters [99,[357][358][359][360][361]. However, many countries still have an aversion to the use of these technologies, which are often complicated by non-science-based ideas [362][363][364]; both public perception and governmental bias around these technologies will need to be addressed, and a more streamlined, long-term approach to these technologies will need to be adopted.…”

Section: Future Prospects and Conclusionmentioning

confidence: 99%

Carotenoids and Apocarotenoids in Planta: Their Role in Plant Development, Contribution to the Flavour and Aroma of Fruits and Flowers, and Their Nutraceutical Benefits

Simkin

2021

Plants

View full text Add to dashboard Cite

Carotenoids and apocarotenoids are diverse classes of compounds found in nature and are important natural pigments, nutraceuticals and flavour/aroma molecules. Improving the quality of crops is important for providing micronutrients to remote communities where dietary variation is often limited. Carotenoids have also been shown to have a significant impact on a number of human diseases, improving the survival rates of some cancers and slowing the progression of neurological illnesses. Furthermore, carotenoid-derived compounds can impact the flavour and aroma of crops and vegetables and are the origin of important developmental, as well as plant resistance compounds required for defence. In this review, we discuss the current research being undertaken to increase carotenoid content in plants and research the benefits to human health and the role of carotenoid derived volatiles on flavour and aroma of fruits and vegetables.

show abstract

Functional Analysis of SBPase Gene Promoter in Transgenic Wheat under Abiotic Stresses

Cited by 7 publications

References 46 publications

Developing specific leaf promoters tools for genetic use in transgenic plants towards food security

Developing specific leaf promoters tools for genetic use in transgenic plants towards food security

Fruit Development in Sweet Cherry

Carotenoids and Apocarotenoids in Planta: Their Role in Plant Development, Contribution to the Flavour and Aroma of Fruits and Flowers, and Their Nutraceutical Benefits

Contact Info

Product

Resources

About