Developing specific leaf promoters tools for genetic use in transgenic plants towards food security

Alotaibi, Saqer S.

doi:10.1016/j.sjbs.2021.05.046

Cited by 2 publications

(3 citation statements)

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“…Similarly, the rice SBPase gene also shows a leaf-specific expression pattern [ 41 ]. Previous studies have shown that the promoters of genes specifically expressed in leaf tissues can be used to achieve the directional expression of target genes in leaf tissues [ 47 ]. For example, the promoters of the key enzymes SBPase and fructose-1,6-bisphosphatase aldolase (FBPA) in the Calvin cycle can be used to achieve the specific expression of target genes in leaf mesophyll cells [ 47 ].…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies have shown that the promoters of genes specifically expressed in leaf tissues can be used to achieve the directional expression of target genes in leaf tissues [ 47 ]. For example, the promoters of the key enzymes SBPase and fructose-1,6-bisphosphatase aldolase (FBPA) in the Calvin cycle can be used to achieve the specific expression of target genes in leaf mesophyll cells [ 47 ]. This study found that GhSBPase was a gene specifically expressed in leaf tissues, indicating that the promoter of this gene may also be important in achieving the directional expression of target genes in leaf tissues.…”

Section: Discussionmentioning

confidence: 99%

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Sequence Characteristics and Expression Analysis of the Gene Encoding Sedoheptulose-1,7-Bisphosphatase, an Important Calvin Cycle Enzyme in Upland Cotton (Gossypium hirsutum L.)

Chao

Dong

et al. 2023

IJMS

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Sedoheptulose-1,7-bisphosphatase (SBPase, EC 3.1.3.37) is a key enzyme in the plant Calvin cycle and one of the main rate-limiting enzymes in the plant photosynthesis pathway. Many studies have demonstrated that the SBPase gene plays an important role in plant photosynthetic efficiency, yield, and stress responses; however, few studies have been conducted on the function and expression of the GhSBPase gene in upland cotton. In this study, our results showed that the coding sequence (CDS) of GhSBPase gene was 1182 bp, encoding a protein with 393 amino acids. The GhSBPase protein had adenosine monophosphate (AMP) binding site and a FIG (FBPase/IMPase/glpX) domain, and had six Cys residues and a CGGT(A/Q)C motif that were involved in redox regulation in plants. Evolutionarily, the GhSBPase protein clustered into the dicotyledon subgroup and was most closely related to the tomato SlSBPase protein. Western-blot analysis further indicated that the GhSBPase gene was indeed the gene encoding the SBPase protein in upland cotton. The GhSBPase protein was localized in chloroplast, which was consistent with its function as a key enzyme in photosynthesis. The GhSBPase gene was specifically highly expressed in leaves, and its expression level was significantly lower in a yellow-green leaf mutant than in the wild type. Moreover, the GhSBPase expression was in response to drought, salt, high- and low-temperature stress, and exhibits different expression patterns. The GhSBPase promoter had the cis-acting elements in response to abiotic stress, phytohormone, and light. In addition, the GhSBPase expression was positively correlated with the chlorophyll fluorescence parameters, suggesting that changes in the expression of the GhSBPase had potential applicability in breeding for enhanced cotton photosynthetic efficiency. These results will help to understand the function of the GhSBPase gene in photosynthesis and the adaptability of plants to external stress and provide important gene information for the high-yield breeding of crops in the future.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Sequence Characteristics and Expression Analysis of the Gene Encoding Sedoheptulose-1,7-Bisphosphatase, an Important Calvin Cycle Enzyme in Upland Cotton (Gossypium hirsutum L.)

Chao

Dong

et al. 2023

IJMS

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“…For example, CDR engineering involves the modification of plant form, such as changing root architecture with less nodal root number and more deep roots in maize [ 176 ], which requires precise control of gene expression by tissue-specific promoters [ 177 ]. Leaf-specific promoters [ 178 ] can be used for driving the expression of genes involved in CO 2 fixation; phloem tissue-specific promoters [ 179 ] can be used for genes involved in phloem-mediated translocation of sugars; and root-specific promoters [ 180 , 181 ] can be used for genes involved in root growth and development. Besides tissue-specific promoters, cell-type-specific promoters [ 182 , 183 ] can be used for high-precision control of the spatial expression pattern of CDR-related genes,…”

Section: Discussionmentioning

confidence: 99%

Biological Parts for Plant Biodesign to Enhance Land-Based Carbon Dioxide Removal

Yang

Liu

et al. 2021

BioDesign Res

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A grand challenge facing society is climate change caused mainly by rising CO2 concentration in Earth’s atmosphere. Terrestrial plants are linchpins in global carbon cycling, with a unique capability of capturing CO2 via photosynthesis and translocating captured carbon to stems, roots, and soils for long-term storage. However, many researchers postulate that existing land plants cannot meet the ambitious requirement for CO2 removal to mitigate climate change in the future due to low photosynthetic efficiency, limited carbon allocation for long-term storage, and low suitability for the bioeconomy. To address these limitations, there is an urgent need for genetic improvement of existing plants or construction of novel plant systems through biosystems design (or biodesign). Here, we summarize validated biological parts (e.g., protein-encoding genes and noncoding RNAs) for biological engineering of carbon dioxide removal (CDR) traits in terrestrial plants to accelerate land-based decarbonization in bioenergy plantations and agricultural settings and promote a vibrant bioeconomy. Specifically, we first summarize the framework of plant-based CDR (e.g., CO2 capture, translocation, storage, and conversion to value-added products). Then, we highlight some representative biological parts, with experimental evidence, in this framework. Finally, we discuss challenges and strategies for the identification and curation of biological parts for CDR engineering in plants.

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Developing specific leaf promoters tools for genetic use in transgenic plants towards food security

Cited by 2 publications

References 50 publications

Sequence Characteristics and Expression Analysis of the Gene Encoding Sedoheptulose-1,7-Bisphosphatase, an Important Calvin Cycle Enzyme in Upland Cotton (Gossypium hirsutum L.)

Sequence Characteristics and Expression Analysis of the Gene Encoding Sedoheptulose-1,7-Bisphosphatase, an Important Calvin Cycle Enzyme in Upland Cotton (Gossypium hirsutum L.)

Biological Parts for Plant Biodesign to Enhance Land-Based Carbon Dioxide Removal

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