Fully automated synthesis of peptides on polyethylene pins

Wiesmüller, Karl‐Heinz; Treffer, Udo; Spohn, Renate; Jung, Günther

doi:10.1007/978-94-011-1470-7_129

Cited by 5 publications

(2 citation statements)

References 2 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Additions to the repertoire of pin synthesis have included fully automated systems to produce peptides on pins. , Development of superior synthesis protocols has also been reported, with a preferred embodiment being Fmoc amino acids activated by the BOP reagent in dimethylacetamide. Heptapeptide libraries can be prepared in only 3 days using this protocol.…”

Section: Peptides-on-pinsmentioning

confidence: 99%

Spatially Addressable Combinatorial Libraries

Pirrung

1997

Chem. Rev.

167

View full text Add to dashboard Cite

ContentsI. Introduction 473 II. Peptides-on-Pins 474 A. Method 474 B. Applications 476 III. Macroscopic DNA Arrays 478 A. Method 478 B. Applications 479 IV. Light-Directed Synthesis 480 A. Background 480 B. Method 480 C. Applications 483 1. Peptides 483 2. DNA 484 V. Peptides on Paper 485 A. Method 485 B. Applications 486 VI. Inorganic Combinatorial Libraries 486 A. Method 486 B. Applications 487 VII. Conclusions 487 VIII. References and Notes 487Michael Pirrung (b. 1955) grew up in the rodeo capital of Texas, Mesquite. After graduating from the University of TexassAustin, he pursued a Ph.D. at UC-Berkeley. He did research on stereoselective aldol condensations with Clayton Heathcock and an independent project on a photochemical total synthesis of isocomene. After a postdoc with Gilbert Stork at Columbia, he took a position at Stanford. His group became known for work on the mechanism of the biosynthesis of ethylene, the plant-ripening hormone. In 1988, he joined a "working group" of scientists meeting in Palo Alto to form a then-new company, Affymax. He established the chemistry program at Affymax, focusing on methods of creating chemical diversity. His work there on light-directed synthesis was recognized with the AAAS-Newcomb Cleveland Prize. In 1990, he moved to Duke University where he is now Professor of Chemistry and Director of the Program in Biological Chemistry. He has published ∼100 peer-reviewed articles and holds several patents. A sabbatical at the Baylor College of Medicine in 1995 resulted in one current interest, chip-based comparison sequencing for mutation detection in highly mutated disease genes.Continuing interests include asymmetric catalysis, synthetic methodology, total synthesis, photochemistry, enzymology, combinatorial chemistry, lightdirected synthesis, and ethylene biosynthesis.

show abstract

Section: Peptides-on-pinsmentioning

confidence: 99%

Spatially Addressable Combinatorial Libraries

Pirrung

1997

Chem. Rev.

167

View full text Add to dashboard Cite

show abstract

“…The support was derivatized with trifluoroacetic acid-labile linkers (4-hydroxymethylphenoxyacetic acid or Rink amide linker 109 ). Wiesmüler et al 110 adapted the multipin approach to a fully automated regime. In a similar manner, Gausepohl and Frank 111 designed a synthesizer utilizing pins with detachable rods.…”

Section: U L T I P L E P E P T I D E S Y N T H E S I S (Mps)mentioning

confidence: 99%

Molecular Diversity and Libraries of Structures: Synthesis and Screening

Rinnová

Lebl²

1996

Collect. Czech. Chem. Commun.

View full text Add to dashboard Cite

Library, or molecular diversity, approaches are a progressive tool in contemporary investigation of biological interactions and drug discovery. All library approaches include certain degree of rationality and cannot be therefore classified as random or irrational techniques. Reviewed techniques are complementary to so called "rational design"; they can serve either as a tool to generate a lead, or, on the other hand, to optimize a "designed" lead by rapid evaluation of structure-activity relationships. Combinatorial approaches are based on either a random or multiple principle which enables production of large number of diverse molecular structures that can be simultaneously screened and evaluated in a biological assay to find an optimal interacting molecule.

show abstract