Full-length sequence, regulation and developmental studies of a second vitellogenin gene from the American dog tick, Dermacentor variabilis

Khalil, Sayed M.S.; Donohue, Kevin V.; Thompson, Deborah; Jeffers, Laura A.; Ananthapadmanaban, Usha; Sonenshine, Daniel E.; Mitchell, Robert D.; Roe, R. Michael

doi:10.1016/j.jinsphys.2010.12.008

Cited by 43 publications

(47 citation statements)

References 48 publications

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“…In insects, the recognition site is generally [R/K]-X-[R/K]-R, although RXXR is also used (Sappington and Raikhel, 1998). This site has also been found in tick Vg sequences, but there is currently no conclusive evidence confirming that tick Vgs described are actually cleaved at these sites (Thompson et al, 2007;Horigane et al, 2010;Boldbaatar et al, 2010;Khalil et al, 2011). Generally, in order for cleavage to occur, the recognition site alone is not sufficient; rather, PC mediated processing occurs only if the recognition site is directly adjacent to a predicted ␤-turn, or is part of an -loop and, as such, is more readily accessible to the PC (Brakch et al, 2000;Rholam and Fahy, 2009).…”

Section: [S/t]h[e/h/p/s/k][e/k]y Repeats and Rmgp80 And Rmvg Have ∼13mentioning

confidence: 99%

“…data from the I. scapularis genome project also show two putative CPs present in that species. Compared to Vgs, such as those in D. variabilis (Thompson et al, 2007;Khalil et al, 2011) and O. moubata (Horigane et al, 2010), which are expressed exclusively in females after they have mated and fed to repletion, CPs are generally expressed in both sexes following feeding (Donohue et al, 2008;Khalil et al, 2013). Comparison of AhVg sequences identified in this work with those of other tick Vgs and CPs, suggests that both sequences are most likely Vgs and not CPs; both are expressed only in females following mating and engorgement, with the main tissue sources being the fat body and midgut.…”

Section: [S/t]h[e/h/p/s/k][e/k]y Repeats and Rmgp80 And Rmvg Have ∼13mentioning

confidence: 99%

“…Due to the similarities between CPs and Vgs in ticks, it is difficult to determine identity based solely upon homology searches. As a result, Khalil et al (2011) proposed that CPs and Vg could be distinguished by (1) examining expression patterns, because Vgs are usually only present in engorged females whereas CP is more ubiquitously expressed throughout feeding and (2) by careful examination of the protein sequence for the highly conserved In situ hybridization of whole tissues showing the localization of AhVg2 transcript in mated females 10 days following engorgement. Deep purple staining indicates AhVg2 mRNA expression.…”

Section: [S/t]h[e/h/p/s/k][e/k]y Repeats and Rmgp80 And Rmvg Have ∼13mentioning

confidence: 99%

“…However, Vg is produced in both the fat body and midgut in a number of tick species (Coons et al, 1989;Rosell and Coons, 1992;Thompson et al, 2007;Boldbaatar et al, 2010;Horigane et al, 2010;Khalil et al, 2011). Following synthesis, Vg is released into the haemolymph and taken up into the developing oocyte by receptormediated endocytosis, where the Vg may be further processed and stored Sappington and Raikhel, 1998;Tufail and Takeda, 2008;Ogihara and Taylor, 2013).…”

Section: Introductionmentioning

confidence: 99%

“…The first complete tick Vg cDNA sequence was reported in 2007 from the American dog tick Dermacentor variabilis (Thompson et al, 2007). Since then, complete Vg sequences have been described from two additional tick species: Ornithodoros moubata (Horigane et al, 2010) and Haemaphysalis longicornis (Boldbaatar et al, 2010), with multiple Vg genes described from both D. variabilis (Khalil et al, 2011) and H. longicornis (Boldbaatar et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

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Molecular characterization of two vitellogenin genes from the tick, Amblyomma hebraeum (Acari: Ixodidae)

Smith¹,

Kaufman²

2014

Ticks and Tick-borne Diseases

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Section: [S/t]h[e/h/p/s/k][e/k]y Repeats and Rmgp80 And Rmvg Have ∼13mentioning

confidence: 99%

Section: [S/t]h[e/h/p/s/k][e/k]y Repeats and Rmgp80 And Rmvg Have ∼13mentioning

confidence: 99%

Section: [S/t]h[e/h/p/s/k][e/k]y Repeats and Rmgp80 And Rmvg Have ∼13mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Molecular characterization of two vitellogenin genes from the tick, Amblyomma hebraeum (Acari: Ixodidae)

Smith¹,

Kaufman²

2014

Ticks and Tick-borne Diseases

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Cross‐reactivity between tick and wasp venom can contribute to frequent wasp sensitization in patients with the α‐Gal syndrome

Kiewiet

Peruško

Grundström

et al. 2022

Clinical & Translational All

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Background: α-Gal syndrome (AGS) is a food allergy with severe delayed allergic reactions, mediated by IgE-reactivity to galactose-α1,3-galactose (α-Gal). AGS is strongly associated with tick bites. An increased incidence of venom sensitization has been found in AGS patients. Here, we evaluated the frequency of wasp sensitization in Swedish AGS patients and the possible cross-reactivity between wasp venom and tick proteins. Methods: Sera from 136 Swedish AGS patients and 29 wasp-positive non-AGS control sera were analyzed for IgE-reactivity against wasp venom (Vespula spp.), the European tick Ixodes ricinus (Streptavidin ImmunoCAP), α-Gal and total IgE by ImmunoCAP. The presence of α-Gal on wasp venom proteins (Vespula vulgaris) was investigated by western blot (WB), and possible cross-reactivity between wasp venom and tick proteins by enzyme-linked immunosorbent assay and WB.Involvement of cross-reactive carbohydrate domains (CCDs) was also assessed.Results: Wasp sensitization was present in 54% of AGS patients, although the IgE levels were low. Wasp sensitized patients had higher IgE levels to α-Gal and total IgE levels compared to non-wasp sensitized AGS patients. α-Gal was not detected in wasp venom, but cross-reactivity between wasp and tick proteins was demonstrated which was not dependent on CCDs. The same cross-reactivity was also observed in the control sera. Furthermore, 17 putative cross-reactive peptides were identified using an in silico approach. Conclusions:For the first time, cross-reactivity between wasp venom and tick proteins has been described. This may be a reason why the majority of Swedish AGS patients, who have all been tick bitten, are also sensitized against wasp. K E Y W O R D Sα-Gal syndrome, cross-reactivity, Ixodes ricinus, red meat allergy, tick, Vespula vulgaris, waspThis is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Purification of vitellin and dynamics of vitellogenesis in the parthenogenetic tick Haemaphysalis longicornis (Acari: Ixodidae)

Yang

et al. 2014

Exp Appl Acarol

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Vitellin (Vt) was purified from eggs of parthenogenetic bush tick Haemaphysalis longicornis by gel filtration and ion exchange chromatography. Our results revealed that only one single Vt existed in parthenogenetic bush tick, and the purified Vt was proved to be a hemoglycolipoprotein consisting of nine polypeptides with molecular weights of 203, 147, 126, 82, 74, 70, 61, 47 and 31 kDa, respectively. Polyclonal antibody and monoclonal antibody against Vt were produced using the purified Vt. The change in vitellogenin (Vg) and Vt levels over time of the parthenogenetic H. longicornis was established, and the Vg content in haemolymph and Vt in ovary at different feeding or engorgement statuses was also determined using a double antibody sandwich enzyme-linked immunosorbent assay. The Vg level in haemolymph was distinctly increased on the day of engorgement (1.785 mg/mL) and continued to increase until 2nd day post-engorgement (5.611 mg/mL). There was a slight decrease in Vg level after 4 days of engorgement, and a second peak was observed on day 2 post-oviposition (10.774 mg/mL). Subsequently, Vg content continuously decreased and reached a low level on the 10th day post-oviposition. The Vt content in ovary continuously increased once the female reached its critical weight (0.024 mg per female), and reached the maximum level on day 2 post-oviposition (1.942 mg per female). Afterwards, Vt content rapidly decreased.

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Full-length sequence, regulation and developmental studies of a second vitellogenin gene from the American dog tick, Dermacentor variabilis

Cited by 43 publications

References 48 publications

Molecular characterization of two vitellogenin genes from the tick, Amblyomma hebraeum (Acari: Ixodidae)

Molecular characterization of two vitellogenin genes from the tick, Amblyomma hebraeum (Acari: Ixodidae)

Cross‐reactivity between tick and wasp venom can contribute to frequent wasp sensitization in patients with the α‐Gal syndrome

Purification of vitellin and dynamics of vitellogenesis in the parthenogenetic tick Haemaphysalis longicornis (Acari: Ixodidae)

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