Molecular characterization of two vitellogenin genes from the tick, Amblyomma hebraeum (Acari: Ixodidae)

Smith, Alexander D.; Kaufman, W. Reuben

doi:10.1016/j.ttbdis.2014.06.001

Cited by 16 publications

(14 citation statements)

References 71 publications

(100 reference statements)

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“…This sexual difference is common in arachnid species. Vg mRNA is also absent in males of D. variabilis (Thompson et al ., ; Khalil et al ., ), A. hebraeum (Smith & Kaufman, ) and H. longicornis (Boldbaatar et al ., ). Of note, PpVg1 , PpVg2 and PpVg3 show different expression patterns after mating and first oviposition.…”

Section: Discussionmentioning

confidence: 97%

“…(Thompson et al, 2007;Khalil et al, 2011). In A. hebraeum, AhVg1 and AhVg2 mRNA are both expressed only in mated adult females after they feed to repletion (Smith & Kaufman, 2014). Compared with PpVg1 and PpVg2, PpVg3 shows distinctive features not only in sequence structure, but also in expression pattern.…”

Section: Discussionmentioning

confidence: 99%

“…Multiple Vg genes are described from Dermacentor variabilis (Acari: Ixodidae) (Thompson et al, 2007;Khalil et al, 2011), Haemaphysalis longicornis (Acari: Ixodidae) (Boldbaatar et al, 2010), Neoseiulus cucumeris (Acari: Phytoseiidae) (Zhao et al, 2014), Varroa destructor (Acari: Varroidae) (Cabrera Cordon et al, 2013), Rhipicephalus microplus (Acari: Ixodidae) (Tirloni et al, 2014), Tetranychus cinnabarinus (Acari: Tetranychidae) (Liu et al, 2016), Rhipicephalus appendiculatus (Acari: Ixodidae) (de Castro et al, 2016), Tropilaelaps mercedesae (Acari, Laelapidae) (Dong et al, 2017) and Tetranychus urticae (Acari: Tetranychidae) (Kawakami et al, 2009). Besides, complete Vg sequences are also described from Ornithodoros moubata (Acari: Argasidae) (Horigane et al, 2010) and Amblyomma hebraeum (Acari: Ixodidae) (Smith & Kaufman, 2014). Although several Vg gene sequences are reported from ticks and mites, the only Vg sequences of spiders are from a single species, Parasteatoda tepidariorum (Araneae: Theridiidae) (accession number: XP_015930210, XP_015930209, XP_015930207, XP_015921257).…”

Section: Introductionmentioning

confidence: 99%

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Molecular characterization and expression of vitellogenin genes from the wolf spider Pardosa pseudoannulata (Araneae: Lycosidae)

Guo

Wang

et al. 2018

Physiol. Entomol.

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The wolf spider Pardosa pseudoannulata (Araneae: Lycosidae) is an important biological control agent against rice pests in the paddy ecosystem. Vitellogenin (Vg) is the precursor of the yolk protein and is crucial for reproduction in P. pseudoannulata. We have identified three full‐length cDNAs encoding vitellogenins. The PpVg1 transcript is 5598 bp long, with an open reading frame (ORF) of 5379 bp encoding a 1792 amino acid protein. The PpVg2 transcript is 5394 bp long, with an ORF of 5205 bp encoding a 1734 amino acid protein. The PpVg3 transcript is 5229 bp long, with an ORF of a 5019 bp encoding a 1672 amino acid protein. Typical domains are found in all PpVgs sequences, including an N‐terminal lipoprotein domain, a DUF1943 domain and the von Willebrand factor type D domain. Phylogenetic analysis indicates that PpVg1, PpVg2 and PpVg3 are grouped with other arachnid Vgs. PpVg1 and PpVg2 are more closely related to Parasteatoda tepidariorum vitellogenin, whereas PpVg3 is segregated into a single clade in the arachnid group. Expression analysis by a quantitative reverse transcriptase‐polymerase chain reaction shows that PpVg1, PpVg2 and PpVg3 are mainly expressed in adult females. Mating elicite an increase in the transcription levels of PpVg1 and PpVg2, and the expression level of PpVg3 is up‐regulated after first oviposition. The present study represents the first report with respect to the molecular characterization and expression patterns for the spider vitellogenin and will greatly facilitate our understanding of the molecular mechanisms of P. pseudoannulata reproduction.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Molecular characterization and expression of vitellogenin genes from the wolf spider Pardosa pseudoannulata (Araneae: Lycosidae)

Guo

Wang

et al. 2018

Physiol. Entomol.

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“…For further interpretation of systemic functions, the D. marginatus dataset was mapped to KEGG pathways. The most enriched 15 pathways in blood meal digestion induced and starvation induced were presented in Currently, proteins in blood meal was the main source of nutrient for oviposition and the energy source for resisting long term-starvation [26,45,46]. Up regulated genes potentially involved in protein metabolism are listed in Table 2 and Supplementary Table 3.…”

Section: Differential Gene Expression Pro Lesmentioning

confidence: 99%

De Novo Assembly and Analysis of Transcriptome of the Dermacentor Marginatus Genes Differentially Expressed After Blood Feeding and Long-term Starvation

Yuan

et al. 2020

Preprint

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Background The Ixodid tick Dermacentor marginatus is a vector of many pathogens wide spread in Eurasia. Study of gene targets of the tick species provides insight to find novel tick protective antigen for drug development and vaccine targets. Methods To obtain a broader picture of gene sequences and changes in expression level, the RNA-seq was performed to obtain the whole-body transcriptome data of D. marginatus adult female ticks after engorgement and long-term starvation. Subsequently, the real-time quantitative PCR (RT-qPCR) was applied to validate the RNA-seq data. Results The RNA-seq produced 30251 unigenes, of which 32% were annotated using Trinity. Gene expression was compared among groups differed by status as newly molted, starved and engorged female adult ticks. Nearly 1/3 of the unigenes in each group were differentially expressed compared to the other two group, and the most numerous were genes encoding proteins involved in catalytic and binding activities and apoptosis. Selected up-regulated differentially expressed genes in each group associated to protein, lipids, carbohydrate and chitin metabolism. Blood feeding and long-term starvation also caused genes differentially expressed in the defense response and antioxidant response. RT-qPCR results indicated 6 differentially expressed transcripts showed similar trends in expression changes with RNA-seq results confirming that the gene expression profiles in transcriptome data is in consistent with RT-qPCR validation. Conclusions Obtaining the sequence information of D. marginatus and characterizing the expression pattern of the genes involved in blood feeding and during starvation would be helpful in understanding molecular physiology of D. marginatus and provides data for anti-tick vaccine and drug development for controlling the tick.

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“…Both blood-feeding and long-term starvation would cause signi cant differential gene expressions. Currently, proteins in blood meal was the main source of nutrient for oviposition and the energy source for resisting long-term starvation [18,19,50]. Up-regulated genes in the three stages potentially involved in protein metabolism are listed in Table 2 and Supplementary Table 5.…”

Section: Differential Gene Expression Pro Lesmentioning

confidence: 99%

De novo assembly and analysis of the transcriptome of the Dermacentor marginatus genes differentially expressed after blood feeding and long-term starvation

Yuan

et al. 2020

Preprint

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Background: The ixodid tick Dermacentor marginatus is a vector of many pathogens wide spread in Eurasia. Studies of gene sequence on many tick species have greatly increased the information on tick protective antigen which might have the potential to function as effective vaccine candidates or drug targets for eco-friendly acaricide development. In the current study, RNA-seq was applied to identify D. marginatus sequences and analyze differentially expressed unigenes.Methods: To obtain a broader picture of gene sequences and changes in expression level, RNA-seq was performed to obtain the whole-body transcriptome data of D. marginatus adult female ticks after engorgement and long-term starvation. Subsequently, the real-time quantitative PCR (RT-qPCR) was applied to validate the RNA-seq data.Results: RNA-seq produced 30,251 unigenes, of which 32% were annotated. Gene expression was compared among groups that differed by status as newly molted, starved and engorged female adult ticks. Nearly 1/3 of the unigenes in each group were differentially expressed compared to the other two groups, and the most numerous were genes encoding proteins involved in catalytic and binding activities and apoptosis. Selected up-regulated differentially expressed genes in each group were associated to protein, lipids, carbohydrate and chitin metabolism. Blood-feeding and long-term starvation also caused genes differentially expressed in the defense response and antioxidant response. RT-qPCR results indicated 6 differentially expressed transcripts showed similar trends in expression changes with RNA-seq results confirming that the gene expression profiles in transcriptome data is in consistent with RT-qPCR validation.Conclusions: Obtaining the sequence information of D. marginatus and characterizing the expression pattern of the genes involved in blood feeding and during starvation would be helpful in understanding molecular physiology of D. marginatus and provides data for anti-tick vaccine and drug development for controlling the tick.

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Molecular characterization of two vitellogenin genes from the tick, Amblyomma hebraeum (Acari: Ixodidae)

Cited by 16 publications

References 71 publications

Molecular characterization and expression of vitellogenin genes from the wolf spider Pardosa pseudoannulata (Araneae: Lycosidae)

Molecular characterization and expression of vitellogenin genes from the wolf spider Pardosa pseudoannulata (Araneae: Lycosidae)

De Novo Assembly and Analysis of Transcriptome of the Dermacentor Marginatus Genes Differentially Expressed After Blood Feeding and Long-term Starvation

De novo assembly and analysis of the transcriptome of the Dermacentor marginatus genes differentially expressed after blood feeding and long-term starvation

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