Full factorial design for optimization, development and validation of HPLC method to determine valsartan in nanoparticles

Abstract

High performance liquid chromatographic method was optimized, developed and validated as per the ICH guidelines. In this study the 20 mM ammonium formate and acetonitrile in the 57:43 ratio were used as mobile phase for the analysis of valsartan. Full factorial design was used to optimize the effect of variable factors. The responses were peak area, tailing factor and number of theoretical plates. The quadratic effect of flow rate and wavelength individually as well as in interaction were most significant (p < 0.0001 and p < 0.0086, respectively) on peak area; the quadratic effect of pH of buffer was also most significant effect (p < 0.0001) on tailing factor (5%) whereas the quadratic effect of flow rate and wavelength individually was significant (p = 0.0006 and p = 0.0265, respectively) on the number of theoretical plates. The high-performance liquid chromatographic separation was performed at the flow rate 1.0 min/mL, UV detector wavelength 250 nm and pH of the buffer 3.0 as optimized parameters using design of experiments. The retention time values of valsartan were found to be 10.177 min. Percent recovery in terms of accuracy for the prepared valsartan nanoparticles was found in the range of 98.57-100.27%.

Formulation and investigation of crushed puffed rice-chitosan-HPMC based polymeric blends as carrier for sustained stomach specific drug delivery of piroxicam using 3(2) Taguchi mathematical design studies

“…One-way ANOVA provided in Minitab-18 was used for statistical validity of the hypothesized model. Significance level was considered at p < 0.05 (Kumar et al, 2015).…”

Simultaneous Determination of Linezolid and Levamisole Hydrochloride in a Fixed Dose Combination

“…18,24 i. Intraday precision Peak area obtained from mixture of linezolid and levamisole standard solution was analysed two times in a day as six independent samples analysis at a time.…”

“…Then the peak area and percent relative standard deviation (% RSD) was calculated. 18,24 ii. Interday precision In this study, the mixture of linezolid and levamisole standard solution was analysed in two different days as six independent samples analysis at a time.…”

“…Limit of detection (LOD) and limit of quantitation (LOQ) were determined using the reported methods 18,[21][22][23][24] and formulae for determination of same are as given below: Where, 'SD' is related to the least standard deviation value obtained in response and 'slope' is obtained from the linearity.…”

A Soft-Drink Experiment using Replicated Full Factorial (RFF) Design

“…For instance a two-level factorial design has been employed to investigate the effect of temperature and altitude on current flow in an integrated circuit by Hicks [4]. Similarly, other valuable contributions towards empirical studies include Pais et al [5], Yasmeen and Siddiqui [6], Kumar et al [7], Ahmed and Suboohi [8], Chen et al [9], and Collin [10].…”