FSCN1 gene polymorphisms: biomarkers for the development and progression of breast cancer

Wang, Chaoqun; Tang, Chih-Hsin; Wang, Yan; Jin, Lai; Wang, Qian; Li, Xiaoni; Hu, Guoqing; Huang, Bifei; Zhao, Yongming; Su, Chen-Ming

doi:10.1038/s41598-017-16196-6

Cited by 44 publications

(39 citation statements)

References 37 publications

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“…Overexpression of FSCN1 promoted migration and invasion of cancer cells [11,12,[14][15][16][25][26][27], and was associated with clinically unfavorable phenotypes in human epithelial cancers including EOC [10,17,27,28] . Nevertheless, the correlation of FSCN1 with EMT was unkown.…”

Section: Discussionmentioning

confidence: 99%

FSCN1 Promotes Epithelial-Mesenchymal Transition Through Increasing Snail1 in Ovarian Cancer Cells

Zhang

Pei

et al. 2018

Cell Physiol Biochem

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Background/Aims: Epithelial-mesenchymal transition (EMT) is one of the key mechanisms mediating cancer progression. Snail1 has a pivotal role in the regulation of EMT, involving the loss of E-cadherin and concomitant upregulation of vimentin, among other biomarkers. We have found FSCN1 promoted EMT in ovarian cancer cells, but the precise mechanism of FSCN1 in EMT process has not been clearly elucidated. Methods: The levels of FSCN1 and snail1 were determined in epithelial ovarian cancer(EOC) specimen and in ovarian cancer cells by RT-qPCR. The changes of EMT makers and effects on snail1 by FSCN1 were examined by overexpression or depletion of FSCN1 in EOC cells by RT-qPCR and western blotting. The invasiveness of the FSCN1-modified EOC cells was examined in transwell assay. Co-immunoprecipitation (IP) was performed to detect the interaction between snail1 and FSCN1 in EOC cells. Results: We found FSCN1 and snail1 significantly increased in EOC, and especially in EOC with metastasis. FSCN1 was positively correlated with snail1 expression at the cellular/histological levels. Moreover, we further showed that FSCN1 physiologically interacted with and increased the levels of snail1 to promote ovarian cancer cell EMT. Conclusion: FSCN1 promote EMT through snail1 in ovarian cancer cells. FSCN1 is an attractive novel target for inhibiting invasion and metastasis of EOC cells.

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Section: Discussionmentioning

confidence: 99%

FSCN1 Promotes Epithelial-Mesenchymal Transition Through Increasing Snail1 in Ovarian Cancer Cells

Zhang

Pei

et al. 2018

Cell Physiol Biochem

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“…Certain SNPs influence susceptibility to breast cancer [7]. The risk of breast cancer is higher in those carrying the BRCA1 and BRCA2 gene mutations [8,9] and genetic polymorphisms such as high-mobility group box protein 1 (HMGB1) and fascin-1 (FSCN1) [10,11].…”

Section: Ivyspringmentioning

confidence: 99%

“…We also enrolled 154 untreated women scheduled for breast cancer surgery at the Affiliated Dongyang Hospital of Wenzhou Medical University (Dongyang, Zhejiang, China) between 2007 and 2017; one tissue specimen was obtained from each participant. Tumor grades were assigned using the Scarff-Bloom-Richardson system and the World Health Organization breast tumor classification criteria were used for pathohistological diagnosis [23] Cases were assigned estrogen receptor (ER), progesterone receptor (PR), human epidermal growth factor receptor 2 (HER2) and Ki-67 status and subtyped as Luminal A (ER-positive [+] and/or PR + , HER2-negative [-], Ki-67 <14%), Luminal B (ER + and/or PR + , HER2 -, Ki-67 ≥14%, ER + and/or PR + , HER2 + ), HER2-enriched (ER -, PR -, HER2 + ), or as triple-negative breast cancer (TNBC; ER -, PR -, HER2 -) [24,25,26]. Clinicopathological information was collected from electronic medical records and at study entry each study participant completed a standardized questionnaire providing sociodemographic data.…”

Section: Participantsmentioning

confidence: 99%

“…Four RETN SNPs were selected for analysis (rs3745367, rs7408174, rs1862513 and rs3219175), as they have previously been found to correlate with breast cancer progression [20] SNPs were genotyped by the TaqMan SNP genotyping assay (Applied Biosystems, Warrington, UK), according to the manufacturer's protocol [27,28]. qPCRs were performed as previously described in a total volume of 20 μL containing a specific Master Mix (10 μL), probes (0.5 μL) and 10 ng of individual genomic DNA [24]. Real-time PCR was performed as previously described, including an initial denaturation step at 95°C for 10 min, then 40 amplification cycles at 95°C for 15 secs and 60°C for 1 min [24,29].…”

Section: Genotype Determinationmentioning

confidence: 99%

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Impacts of RETN genetic polymorphism on breast cancer development

et al. 2020

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The adipokine resistin is linked with obesity, inflammation and various cancers, including breast cancer. This study sought to determine whether certain polymorphisms in the gene encoding resistin, RETN, increase the risk of breast cancer susceptibility. We analyzed levels of resistin expression in breast cancer tissue and samples from The Cancer Genome Atlas database. We also examined associations between four RETN single nucleotide polymorphisms (SNPs; rs3745367, rs7408174, rs1862513 and rs3219175) and breast cancer susceptibility in 515 patients with breast cancer and 541 healthy women without cancer. Compared with wild-type (GG) carriers, those carrying the AG genotype of the RETN SNP rs3219175 and those carrying at least one A allele in the SNP rs3219175 had a higher chance of developing breast cancer (adjusted odds ratio, AOR: 1.295, 95% confidence intervals, CI: 1.065-1.575 and 2.202, 1.701-2.243, respectively). When clinical aspects and the RETN SNP rs7408174 were examined in the breast cancer cohort, the CT genotype was linked to late-stage disease, while women with luminal A disease and at least one C allele were likely to progress to stage III/IV disease and to develop highly pathological grade III disease. Moreover, resistin-positive individuals were at greater risk than resistin-negative individuals for developing pathological grade III disease (OR: 5.020; 95% CI: 1.380-18.259). This study details risk associations between resistin and RETN SNPs in breast cancer susceptibility in Chinese Han women.

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“…Five Angpt2 SNP probes were purchased from Thermo Fisher Scientific Inc. (USA) and assessment of allelic discrimination for Angpt2 SNPs was conducted using a QuantStudio TM 5 Real-Time PCR system (Applied Biosystems, CA, USA), according to the manufacturer's instructions. Data were further analyzed with QuantStudio™ Design & Analysis Software (Applied Biosystems) and analytic statistics were compiled with clinical data [19]. PCR genotyping was performed in a total volume of 10 μL, containing 20-70 ng genomic DNA, 1 U Taqman Genotyping Master Mix (Applied Biosystems, Foster City, CA, USA), and 0.25 μL probes.…”

Section: Genotyping By Real-time Pcrmentioning

confidence: 99%

Angiopoietin-2 gene polymorphisms are biomarkers for the development and progression of colorectal cancer in Han Chinese

Du¹,

Tang²,

Li³

et al. 2020

Int. J. Med. Sci.

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Colorectal cancer (CRC) is one of the most common cancers in Han Chinese and is characterized by low rates of early diagnosis and poor survival rates. Angiopoietin-2 (Ang2), an endothelial tyrosine kinase, is involved in CRC progression, but little is known about the association between single nucleotide polymorphisms (SNPs) and diagnosis or prognosis of CRC. This study reports on the association between 5 SNPs of the Angpt2 gene (rs2442598, rs734701, rs1823375, 11137037, and rs12674822) and CRC susceptibility as well as clinical outcomes in 379 patients with CRC and in 1,043 cancer-free healthy controls. Carriers of the CG allele at rs1823375 and those with the GT+TT allele of the variant rs12674822 were at greater risk of CRC than their respective wild-type counterparts. Moreover, carriers of the GT or GT+TT allele in rs12674822 were significantly more likely to have tumor involvement in both the colon and rectum compared with wild-type (GG) carriers, while 5-year progression-free survival was also significantly worse in those carrying the GT+TT allele in rs12674822 compared with wild-type carriers. Our study is the first to describe correlations between Angpt2 polymorphisms and CRC development and progression in people of Chinese Han ethnicity.

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FSCN1 gene polymorphisms: biomarkers for the development and progression of breast cancer

Cited by 44 publications

References 37 publications

FSCN1 Promotes Epithelial-Mesenchymal Transition Through Increasing Snail1 in Ovarian Cancer Cells

FSCN1 Promotes Epithelial-Mesenchymal Transition Through Increasing Snail1 in Ovarian Cancer Cells

Impacts of RETN genetic polymorphism on breast cancer development

Angiopoietin-2 gene polymorphisms are biomarkers for the development and progression of colorectal cancer in Han Chinese

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